Caspase 2 Mouse Monoclonal Antibody

Product:	Caspase 2 Mouse Monoclonal Antibody
Catalog:	BF8401
Description:	Mouse monoclonal antibody to Caspase 2
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Rabbit
Mol.Wt.:	49kDa; 51kD(Calculated).
Uniprot:	P42575

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
50ul	$250	In stock
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Monoclonal [AFfirm8401]

Specificity:

Caspase 2 Mouse Monoclonal Antibody detects endogenous levels of total Caspase 2

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CASP 2; CASP-2; Casp2; CASP2_HUMAN; Caspase 2; Caspase 2 apoptosis related cysteine peptidase; Caspase-2 subunit p12; Caspase2; ICH 1; ICH 1 protease; ICH 1L; ICH1; ICH1 protease; ICH1L; NEDD-2; NEDD2; Neural precursor cell expressed developmentally down-regulated protein 2; PPP1R57; Protease ICH-1; Protein phosphatase 1 regulatory subunit 57;

Immunogens

Immunogen:

Uniprot:

P42575(CASP2_HUMAN) >>Visit HPA database.

Gene(ID):

CASP2(835)

Expression:

P42575 CASP2_HUMAN:

Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle.

Sequence:

P42575 CASP2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAPSAGSWSTFQHKELMAADRGRRILGVCGMHPHHQETLKKNRVVLAKQLLLSELLEHLLEKDIITLEMRELIQAKVGSFSQNVELLNLLPKRGPQAFDAFCEALRETKQGHLEDMLLTTLSGLQHVLPPLSCDYDLSLPFPVCESCPLYKKLRLSTDTVEHSLDNKDGPVCLQVKPCTPEFYQTHFQLAYRLQSRPRGLALVLSNVHFTGEKELEFRSGGDVDHSTLVTLFKLLGYDVHVLCDQTAQEMQEKLQNFAQLPAHRVTDSCIVALLSHGVEGAIYGVDGKLLQLQEVFQLFDNANCPSLQNKPKMFFIQACRGDETDRGVDQQDGKNHAGSPGCEESDAGKEKLPKMRLPTRSDMICGYACLKGTAAMRNTKRGSWYIEALAQVFSERACDMHVADMLVKVNALIKDREGYAPGTEFHRCKEMSEYCSTLCRHLYLFPGHPPT

PTMs - P42575 As Substrate

P42575

Site	PTM Type	Enzyme	Source
A2	Acetylation		Uniprot
S8	Phosphorylation		Uniprot
K15	Ubiquitination		Uniprot
R22	Methylation		Uniprot
K41	Ubiquitination		Uniprot
K77	Sumoylation		Uniprot
K77	Ubiquitination		Uniprot
K93	Ubiquitination		Uniprot
R107	Methylation		Uniprot
S139	Phosphorylation	P78527 (PRKDC)	Uniprot
Y151	Phosphorylation		Uniprot
K153	Ubiquitination		Uniprot
S157	Phosphorylation	P68400 (CSNK2A1)	Uniprot
T160	Phosphorylation		Uniprot
S164	Phosphorylation		Uniprot
K168	Ubiquitination		Uniprot
K177	Ubiquitination		Uniprot
K214	Ubiquitination		Uniprot
K254	Ubiquitination		Uniprot
K335	Ubiquitination		Uniprot
S340	Phosphorylation		Uniprot
Y368	Phosphorylation		Uniprot
T374	Phosphorylation		Uniprot
K409	Ubiquitination		Uniprot
K415	Ubiquitination		Uniprot
Y420	Phosphorylation		Uniprot
K430	Ubiquitination		Uniprot

Research Backgrounds

P42575_CASP2_HUMAN

Function:

Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. Associates with PIDD1 and CRADD to form the PIDDosome, a complex that activates CASP2 and triggers apoptosis in response to genotoxic stress.

PTMs:

The mature protease can process its own propeptide, but not that of other caspases.

Tissue Specificity:

Subunit Structure:

Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a p18 subunit and a p12 subunit. Forms a complex named the PIDDosome with PIDD1 and CRADD. Interacts with NOL3 (via CARD domain); inhibits CASP2 activity in a phosphorylation-dependent manner.

Family&Domains:

The CARD domain mediates a direct interaction with CRADD.

Belongs to the peptidase C14A family.

Research Fields

· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)

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AFfirm™ Caspase 2 Mouse Monoclonal Antibody - #BF8401