AFfirm™ MSK2 Mouse Monoclonal Antibody - #BF8398
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Fold/Unfold
90 kDa ribosomal protein S6 kinase 4; EC 2.7.11.1; KS6A4_HUMAN; Mitogen and stress activated protein kinase 2; Nuclear mitogen and stress activated protein kinase 2; Nuclear mitogen- and stress-activated protein kinase 2; Ribosomal protein kinase B; Ribosomal protein S6 kinase 90kD polypeptide 4; Ribosomal protein S6 kinase 90kDa polypeptide 4; Ribosomal protein S6 kinase alpha 4; Ribosomal protein S6 kinase alpha-4; RPS6KA4; RSKB; S6K-alpha-4;
Immunogens
- O75676 KS6A4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGDEDDDESCAVELRITEANLTGHEEKVSVENFELLKVLGTGAYGKVFLVRKAGGHDAGKLYAMKVLRKAALVQRAKTQEHTRTERSVLELVRQAPFLVTLHYAFQTDAKLHLILDYVSGGEMFTHLYQRQYFKEAEVRVYGGEIVLALEHLHKLGIIYRDLKLENVLLDSEGHIVLTDFGLSKEFLTEEKERTFSFCGTIEYMAPEIIRSKTGHGKAVDWWSLGILLFELLTGASPFTLEGERNTQAEVSRRILKCSPPFPPRIGPVAQDLLQRLLCKDPKKRLGAGPQGAQEVRNHPFFQGLDWVALAARKIPAPFRPQIRSELDVGNFAEEFTRLEPVYSPPGSPPPGDPRIFQGYSFVAPSILFDHNNAVMTDGLEAPGAGDRPGRAAVARSAMMQDSPFFQQYELDLREPALGQGSFSVCRRCRQRQSGQEFAVKILSRRLEANTQREVAALRLCQSHPNVVNLHEVHHDQLHTYLVLELLRGGELLEHIRKKRHFSESEASQILRSLVSAVSFMHEEAGVVHRDLKPENILYADDTPGAPVKIIDFGFARLRPQSPGVPMQTPCFTLQYAAPELLAQQGYDESCDLWSLGVILYMMLSGQVPFQGASGQGGQSQAAEIMCKIREGRFSLDGEAWQGVSEEAKELVRGLLTVDPAKRLKLEGLRGSSWLQDGSARSSPPLRTPDVLESSGPAVRSGLNATFMAFNRGKREGFFLKSVENAPLAKRRKQKLRSATASRRGSPAPANPGRAPVASKGAPRRANGPLPPS
PTMs - O75676 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T22 | Phosphorylation | Uniprot | |
| K27 | Ubiquitination | Uniprot | |
| S29 | Phosphorylation | Uniprot | |
| K37 | Ubiquitination | Uniprot | |
| T41 | Phosphorylation | Uniprot | |
| Y44 | Phosphorylation | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| S87 | Phosphorylation | Uniprot | |
| K134 | Ubiquitination | Uniprot | |
| Y141 | Phosphorylation | Uniprot | |
| S183 | Phosphorylation | Uniprot | |
| K184 | Ubiquitination | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| T194 | Phosphorylation | Uniprot | |
| S196 | Phosphorylation | Q16539 (MAPK14) , O75676 (RPS6KA4) | Uniprot |
| K313 | Ubiquitination | Uniprot | |
| S324 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| Y342 | Phosphorylation | Uniprot | |
| S343 | Phosphorylation | Q16539 (MAPK14) | Uniprot |
| S347 | Phosphorylation | Q16539 (MAPK14) | Uniprot |
| Y359 | Phosphorylation | Uniprot | |
| S360 | Phosphorylation | Q16539 (MAPK14) | Uniprot |
| S365 | Phosphorylation | Uniprot | |
| T376 | Phosphorylation | Uniprot | |
| K440 | Ubiquitination | Uniprot | |
| S502 | Phosphorylation | Uniprot | |
| K532 | Ubiquitination | Uniprot | |
| T542 | Phosphorylation | Uniprot | |
| K548 | Ubiquitination | Uniprot | |
| T568 | Phosphorylation | Q16539 (MAPK14) | Uniprot |
| S634 | Phosphorylation | Uniprot | |
| K648 | Ubiquitination | Uniprot | |
| K661 | Ubiquitination | Uniprot | |
| K664 | Sumoylation | Uniprot | |
| K664 | Ubiquitination | Uniprot | |
| S678 | Phosphorylation | Uniprot | |
| S681 | Phosphorylation | Uniprot | |
| S682 | Phosphorylation | Uniprot | |
| T687 | Phosphorylation | Uniprot | |
| K720 | Ubiquitination | Uniprot | |
| S721 | Phosphorylation | Uniprot | |
| K729 | Ubiquitination | Uniprot | |
| S737 | Phosphorylation | Uniprot | |
| R743 | Methylation | Uniprot | |
| S745 | Phosphorylation | Uniprot | |
| S772 | Phosphorylation | Uniprot |
PTMs - O75676 As Enzyme
Research Backgrounds
Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factor RELA, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors.
Ser-343 and Thr-568 phosphorylation is required for kinase activity. Ser-343 and Ser-196 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain. Phosphorylated at Ser-343, Thr-568 and Thr-687 by MAPK1/ERK2, MAPK3/ERK1 and MAPK14/p38-alpha. Autophosphorylated at Ser-737 and Ser-745 by the N-terminal kinase domain (By similarity).
Nucleus.
Forms a complex with either MAPK1/ERK2 or MAPK3/ERK1 in quiescent cells which transiently dissociates following mitogenic stimulation. Also associates with MAPK14/p38-alpha. Activated RPS6KA4 associates with and phosphorylates the NF-kappa-B p65 subunit RELA.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Research Fields
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)
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