PHGDH Mouse Monoclonal Antibody | Affinity Biosciences

Product:	PHGDH Mouse Monoclonal Antibody
Catalog:	BF8396
Description:	Mouse monoclonal antibody to PHGDH
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	57kDa; 57kD(Calculated).
Uniprot:	O43175

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
50ul	$250	In stock
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Monoclonal [AFfirm8396]

Specificity:

PHGDH Mouse Monoclonal Antibody detects endogenous levels of total PHGDH

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

3 PGDH; 3-PGDH; 3-phosphoglycerate dehydrogenase; 3PGDH; D-3-phosphoglycerate dehydrogenase; EC 1.1.1.95; Epididymis secretory protein Li 113; HEL S 113; NLS; NLS1; PDG; PGAD; PGD; PGDH; PGDH3; Phgdh; PHGDHD; Phosphoglycerate dehydrogenase; SERA; SERA_HUMAN;

Immunogens

Immunogen:

Uniprot:

O43175(SERA_HUMAN) >>Visit HPA database.

Gene(ID):

PHGDH(26227)

Sequence:

O43175 SERA_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAFANLRKVLISDSLDPCCRKILQDGGLQVVEKQNLSKEELIAELQDCEGLIVRSATKVTADVINAAEKLQVVGRAGTGVDNVDLEAATRKGILVMNTPNGNSLSAAELTCGMIMCLARQIPQATASMKDGKWERKKFMGTELNGKTLGILGLGRIGREVATRMQSFGMKTIGYDPIISPEVSASFGVQQLPLEEIWPLCDFITVHTPLLPSTTGLLNDNTFAQCKKGVRVVNCARGGIVDEGALLRALQSGQCAGAALDVFTEEPPRDRALVDHENVISCPHLGASTKEAQSRCGEEIAVQFVDMVKGKSLTGVVNAQALTSAFSPHTKPWIGLAEALGTLMRAWAGSPKGTIQVITQGTSLKNAGNCLSPAVIVGLLKEASKQADVNLVNAKLLVKEAGLNVTTSHSPAAPGEQGFGECLLAVALAGAPYQAVGLVQGTTPVLQGLNGAVFRPEVPLRRDLPLLLFRTQTSDPAMLPTMIGLLAEAGVRLLSYQTSLVSDGETWHVMGISSLLPSLEAWKQHVTEAFQFHF

PTMs - O43175 As Substrate

O43175

Site	PTM Type	Enzyme	Source
R7	Methylation		Uniprot
K8	Ubiquitination		Uniprot
S14	Phosphorylation		Uniprot
K21	Sumoylation		Uniprot
K21	Ubiquitination		Uniprot
K33	Acetylation		Uniprot
K33	Ubiquitination		Uniprot
K38	Ubiquitination		Uniprot
S55	Phosphorylation	Q05513 (PRKCZ)	Uniprot
T57	Phosphorylation	Q05513 (PRKCZ)	Uniprot
K58	Ubiquitination		Uniprot
T60	Phosphorylation		Uniprot
K69	Ubiquitination		Uniprot
R75	Methylation		Uniprot
T78	Phosphorylation	Q05513 (PRKCZ)	Uniprot
T89	Phosphorylation		Uniprot
S105	Phosphorylation		Uniprot
T125	Phosphorylation		Uniprot
S127	Phosphorylation		Uniprot
K129	Ubiquitination		Uniprot
K137	Ubiquitination		Uniprot
T141	Phosphorylation		Uniprot
K146	Ubiquitination		Uniprot
T147	Phosphorylation		Uniprot
R236	Methylation		Uniprot
R247	Methylation		Uniprot
C281	S-Nitrosylation		Uniprot
K289	Acetylation		Uniprot
K289	Ubiquitination		Uniprot
K308	Ubiquitination		Uniprot
S326	Phosphorylation		Uniprot
S349	Phosphorylation		Uniprot
K351	Ubiquitination		Uniprot
T353	Phosphorylation		Uniprot
T358	Phosphorylation		Uniprot
S362	Phosphorylation		Uniprot
K364	Ubiquitination		Uniprot
C369	S-Nitrosylation		Uniprot
S371	Phosphorylation		Uniprot
K380	Ubiquitination		Uniprot
K384	Ubiquitination		Uniprot
K394	Ubiquitination		Uniprot
K398	Ubiquitination		Uniprot
S473	Phosphorylation		Uniprot
T480	Phosphorylation		Uniprot
K522	Ubiquitination		Uniprot

Research Backgrounds

O43175_SERA_HUMAN

Function:

Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate and the reversible oxidation of (S)-malate to oxaloacetate.

Subunit Structure:

Homotetramer.

Family&Domains:

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Research Fields

· Metabolism > Amino acid metabolism > Glycine, serine and threonine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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AFfirm™ PHGDH Mouse Monoclonal Antibody - #BF8396