AFfirm™ KSR2 Mouse Monoclonal Antibody - #BF8389

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Product: KSR2 Mouse Monoclonal Antibody
Catalog: BF8389
Description: Mouse monoclonal antibody to KSR2
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.: 115kDa; 108kD(Calculated).
Uniprot: Q6VAB6

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 50ul $250 In stock
 100ul $350 In stock
 200ul $450 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
WB 1:500-1:3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Monoclonal [AFfirm8389]
Specificity:
KSR2 Mouse Monoclonal Antibody detects endogenous levels of total KSR2
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ25965; hKSR 2; hKSR2; Kinase suppressor of Ras 2; KSR 2; Ksr2; KSR2_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q6VAB6(KSR2_HUMAN) >>Visit HPA database.

Gene(ID):
KSR2(283455)
Expression:
Q6VAB6 KSR2_HUMAN:

Mainly expressed in brain and kidney.

Sequence:
MDEENMTKSEEQQPLSLQKALQQCELVQNMIDLSISNLEGLRTKCATSNDLTQKEIRTLESKLVKYFSRQLSCKKKVALQERNAELDGFPQLRHWFRIVDVRKEVLEEISPGQLSLEDLLEMTDEQVCETVEKYGANREECARLNASLSCLRNVHMSGGNLSKQDWTIQWPTTETGKENNPVCPPEPTPWIRTHLSQSPRVPSKCVQHYCHTSPTPGAPVYTHVDRLTVDAYPGLCPPPPLESGHRSLPPSPRQRHAVRTPPRTPNIVTTVTPPGTPPMRKKNKLKPPGTPPPSSRKLIHLIPGFTALHRSKSHEFQLGHRVDEAHTPKAKKKSKPLNLKIHSSVGSCENIPSQQRSPLLSERSLRSFFVGHAPFLPSTPPVHTEANFSANTLSVPRWSPQIPRRDLGNSIKHRFSTKYWMSQTCTVCGKGMLFGLKCKNCKLKCHNKCTKEAPPCHLLIIHRGDPARLVRTESVPCDINNPLRKPPRYSDLHISQTLPKTNKINKDHIPVPYQPDSSSNPSSTTSSTPSSPAPPLPPSATPPSPLHPSPQCTRQQKNFNLPASHYYKYKQQFIFPDVVPVPETPTRAPQVILHPVTSNPILEGNPLLQIEVEPTSENEEVHDEAEESEDDFEEMNLSLLSARSFPRKASQTSIFLQEWDIPFEQLEIGELIGKGRFGQVYHGRWHGEVAIRLIDIERDNEDQLKAFKREVMAYRQTRHENVVLFMGACMSPPHLAIITSLCKGRTLYSVVRDAKIVLDVNKTRQIAQEIVKGMGYLHAKGILHKDLKSKNVFYDNGKVVITDFGLFSISGVLQAGRREDKLRIQNGWLCHLAPEIIRQLSPDTEEDKLPFSKHSDVFALGTIWYELHAREWPFKTQPAEAIIWQMGTGMKPNLSQIGMGKEISDILLFCWAFEQEERPTFTKLMDMLEKLPKRNRRLSHPGHFWKSAEL

PTMs - Q6VAB6 As Substrate

Site PTM Type Enzyme
S34 Phosphorylation
K44 Ubiquitination
T260 Phosphorylation
T264 Phosphorylation
T269 Phosphorylation
T270 Phosphorylation
T272 Phosphorylation
T276 Phosphorylation
K281 Acetylation
T290 Phosphorylation
S294 Phosphorylation
T306 Phosphorylation
S311 Phosphorylation
S313 Phosphorylation
S347 Phosphorylation
S394 Phosphorylation
S399 Phosphorylation
S474 Phosphorylation
S490 Phosphorylation
T497 Phosphorylation
Y566 Phosphorylation
Y794 Phosphorylation
T802 Phosphorylation
S808 Phosphorylation
K923 Ubiquitination
S939 Phosphorylation

PTMs - Q6VAB6 As Enzyme

Substrate Site Source
Q02750 (MAP2K1) S24 Uniprot
Q02750 (MAP2K1) S72 Uniprot

Research Backgrounds

Function:

Location-regulated scaffold connecting MEK to RAF. Has very low protein kinase activity and can phosphorylate MAP2K1 at several Ser and Thr residues with very low efficiency (in vitro). Acts as MAP2K1/MEK1-dependent allosteric activator of BRAF; upon binding to MAP2K1/MEK1, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1. Interaction with BRAF enhances KSR2-mediated phosphorylation of MAP2K1 (in vitro). Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production.

PTMs:

Phosphorylated on Ser-474 by MARK3.

Subcellular Location:

Cytoplasm. Membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Mainly expressed in brain and kidney.

Subunit Structure:

Heterodimerizes (via N-terminus) with BRAF (via N-terminus) in a MAP2K1/MEK1-dependent manner. Interacts with BRAF; this increases the low intrinsic protein kinase activity of KSR2. Interacts with MAP2K1, forming a heterodimer that can dimerize to form a heterotetramer. Interacts with MAP3K8, MAPK, RAS and RAF.

Family&Domains:

The protein kinase domain is predicted to be catalytically inactive and seems to have very low intrinsic kinase activity. This low kinase activity can be increased by interaction with BRAF.

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.

Research Fields

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

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