AFfirm™ c-Myb Mouse Monoclonal Antibody - #BF8298

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Product: c-Myb Mouse Monoclonal Antibody
Catalog: BF8298
Description: Mouse monoclonal antibody to c-Myb
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 80kDa; 72kD(Calculated).
Uniprot: P10242

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   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
WB 1:500-1:3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Monoclonal [AFfirm8298]
Specificity:
c-Myb Mouse Monoclonal Antibody detects endogenous levels of total c-Myb
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C myb; C myb protein; Cmyb; MYB; Myb proto oncogene; V myb myeloblastosis viral oncogene homolog;

Immunogens

Immunogen:

A synthesized peptide derived from human c-Myb, corresponding to a region within C-terminal amino acids.

Uniprot:

P10242(MYB_HUMAN) >>Visit HPA database.

Gene(ID):
MYB(4602)
Description:
Myb is a transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
Sequence:
MARRPRHSIYSSDEDDEDFEMCDHDYDGLLPKSGKRHLGKTRWTREEDEKLKKLVEQNGTDDWKVIANYLPNRTDVQCQHRWQKVLNPELIKGPWTKEEDQRVIELVQKYGPKRWSVIAKHLKGRIGKQCRERWHNHLNPEVKKTSWTEEEDRIIYQAHKRLGNRWAEIAKLLPGRTDNAIKNHWNSTMRRKVEQEGYLQESSKASQPAVATSFQKNSHLMGFAQAPPTAQLPATGQPTVNNDYSYYHISEAQNVSSHVPYPVALHVNIVNVPQPAAAAIQRHYNDEDPEKEKRIKELELLLMSTENELKGQQVLPTQNHTCSYPGWHSTTIADHTRPHGDSAPVSCLGEHHSTPSLPADPGSLPEESASPARCMIVHQGTILDNVKNLLEFAETLQFIDSFLNTSSNHENSDLEMPSLTSTPLIGHKLTVTTPFHRDQTVKTQKENTVFRTPAIKRSILESSPRTPTPFKHALAAQEIKYGPLKMLPQTPSHLVEDLQDVIKQESDESGIVAEFQENGPPLLKKIKQEVESPTDKSGNFFCSHHWEGDSLNTQLFTQTSPVADAPNILTSSVLMAPASEDEDNVLKAFTVPKNRSLASPLQPCSSTWEPASCGKMEEQMTSSSQARKYVNAFSARTLVM

PTMs - P10242 As Substrate

Site PTM Type Enzyme
S11 Phosphorylation P68400 (CSNK2A1)
S12 Phosphorylation P68400 (CSNK2A1)
K53 Ubiquitination
Y69 Phosphorylation
K84 Ubiquitination
K92 Ubiquitination
K97 Ubiquitination
K109 Ubiquitination
K113 Ubiquitination
S116 Phosphorylation P17612 (PRKACA)
K120 Ubiquitination
C130 S-Nitrosylation
K143 Sumoylation
K143 Ubiquitination
K160 Ubiquitination
K171 Ubiquitination
K182 Ubiquitination
K192 Ubiquitination
K204 Ubiquitination
K291 Ubiquitination
K293 Ubiquitination
K296 Ubiquitination
T420 Phosphorylation
T433 Phosphorylation
K442 Ubiquitination
K445 Acetylation
K445 Ubiquitination
T452 Phosphorylation
R457 Methylation
S463 Phosphorylation
R465 Methylation
T466 Phosphorylation
T468 Phosphorylation
K471 Acetylation
K471 Methylation
K471 Ubiquitination
K480 Acetylation
K480 Sumoylation
K480 Ubiquitination
K485 Acetylation
K485 Ubiquitination
K503 Sumoylation
K527 Sumoylation
S532 Phosphorylation P27361 (MAPK3)
T534 Phosphorylation
K593 Ubiquitination
S599 Phosphorylation
K615 Ubiquitination
T621 Phosphorylation
S624 Phosphorylation
K628 Ubiquitination

Research Backgrounds

Function:

Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.

PTMs:

Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.

Phosphorylated by NLK on multiple sites, which induces proteasomal degradation.

Phosphorylated by HIPK1. This phosphorylation reduces MYB transcription factor activity but not MYB protein levels.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Binds MYBBP1A. Interacts with HIPK2, MAF and NLK (By similarity). Binds to HIPK1.

Family&Domains:

Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression.

Research Fields

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

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