AFfirm™ MCM3 Mouse Monoclonal Antibody - #BF8369
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Fold/Unfold
Cervical cancer proto oncogene 5; DNA polymerase alpha holoenzyme associated P1; DNA polymerase alpha holoenzyme associated protein P1; DNA polymerase alpha holoenzyme-associated protein P1; DNA replication factor MCM3; DNA replication licensing factor mcm3; HCC 5; HCC5; hRlf beta subunit; Human cervical cancer proto oncogene 5; MCM 3; mcm3; MCM3 minichromosome maintenance deficient 3; MCM3_HUMAN; MGC1157; Minichromosome maintenance complex component 3; Minichromosome maintenance deficient 3; Minichromosome maintenance protein 3; P1 h; P1 MCM3; P1 Protein; P1-MCM3; P1.h; p102; P102 protein; Replication licensing factor beta subunit; RLF beta subunit; RLF subunit beta; RLFB;
Immunogens
- P25205 MCM3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGTVVLDDVELREAQRDYLDFLDDEEDQGIYQSKVRELISDNQYRLIVNVNDLRRKNEKRANRLLNNAFEELVAFQRALKDFVASIDATYAKQYEEFYVGLEGSFGSKHVSPRTLTSCFLSCVVCVEGIVTKCSLVRPKVVRSVHYCPATKKTIERRYSDLTTLVAFPSSSVYPTKDEENNPLETEYGLSVYKDHQTITIQEMPEKAPAGQLPRSVDVILDDDLVDKAKPGDRVQVVGTYRCLPGKKGGYTSGTFRTVLIACNVKQMSKDAQPSFSAEDIAKIKKFSKTRSKDIFDQLAKSLAPSIHGHDYVKKAILCLLLGGVERDLENGSHIRGDINILLIGDPSVAKSQLLRYVLCTAPRAIPTTGRGSSGVGLTAAVTTDQETGERRLEAGAMVLADRGVVCIDEFDKMSDMDRTAIHEVMEQGRVTIAKAGIHARLNARCSVLAAANPVYGRYDQYKTPMENIGLQDSLLSRFDLLFIMLDQMDPEQDREISDHVLRMHRYRAPGEQDGDAMPLGSAVDILATDDPNFSQEDQQDTQIYEKHDNLLHGTKKKKEKMVSAAFMKKYIHVAKIIKPVLTQESATYIAEEYSRLRSQDSMSSDTARTSPVTARTLETLIRLATAHAKARMSKTVDLQDAEEAVELVQYAYFKKVLEKEKKRKKRSEDESETEDEEEKSQEDQEQKRKRRKTRQPDAKDGDSYDPYDFSDTEEEMPQVHTPKTADSQETKESQKVELSESRLKAFKVALLDVFREAHAQSIGMNRLTESINRDSEEPFSSVEIQAALSKMQDDNQVMVSEGIIFLI
PTMs - P25205 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| T4 | Phosphorylation | Uniprot | |
| Y19 | Phosphorylation | Uniprot | |
| Y32 | Phosphorylation | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| K35 | Ubiquitination | Uniprot | |
| Y45 | Phosphorylation | Uniprot | |
| R55 | Methylation | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| S112 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K140 | Ubiquitination | Uniprot | |
| S144 | Phosphorylation | Uniprot | |
| C148 | S-Nitrosylation | Uniprot | |
| K152 | Acetylation | Uniprot | |
| K152 | Ubiquitination | Uniprot | |
| T154 | Phosphorylation | Uniprot | |
| Y159 | Phosphorylation | Uniprot | |
| S160 | Phosphorylation | P53355 (DAPK1) , O14757 (CHEK1) | Uniprot |
| T163 | Phosphorylation | Uniprot | |
| T164 | Phosphorylation | Uniprot | |
| S170 | Phosphorylation | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| T176 | Phosphorylation | Uniprot | |
| K177 | Ubiquitination | Uniprot | |
| T198 | Phosphorylation | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| K228 | Ubiquitination | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| R234 | Methylation | Uniprot | |
| T240 | Phosphorylation | Uniprot | |
| K248 | Sumoylation | Uniprot | |
| K248 | Ubiquitination | Uniprot | |
| T252 | Phosphorylation | Uniprot | |
| T255 | Phosphorylation | Uniprot | |
| T258 | Phosphorylation | Uniprot | |
| C263 | S-Nitrosylation | Uniprot | |
| K266 | Sumoylation | Uniprot | |
| K266 | Ubiquitination | Uniprot | |
| K270 | Ubiquitination | Uniprot | |
| S275 | Phosphorylation | Uniprot | |
| K283 | Ubiquitination | Uniprot | |
| S292 | Phosphorylation | Uniprot | |
| K293 | Ubiquitination | Uniprot | |
| K301 | Ubiquitination | Uniprot | |
| Y312 | Phosphorylation | Uniprot | |
| K314 | Ubiquitination | Uniprot | |
| K315 | Ubiquitination | Uniprot | |
| K351 | Ubiquitination | Uniprot | |
| R371 | Methylation | Uniprot | |
| T383 | Phosphorylation | Uniprot | |
| T384 | Phosphorylation | Uniprot | |
| K413 | Ubiquitination | Uniprot | |
| R419 | Methylation | Uniprot | |
| K435 | Ubiquitination | Uniprot | |
| K463 | Ubiquitination | Uniprot | |
| S474 | Phosphorylation | Uniprot | |
| S535 | Phosphorylation | Q13535 (ATR) , Q13315 (ATM) | Uniprot |
| Y545 | Phosphorylation | Uniprot | |
| T555 | Phosphorylation | Uniprot | |
| K556 | Acetylation | Uniprot | |
| K556 | Ubiquitination | Uniprot | |
| K559 | Acetylation | Uniprot | |
| K561 | Ubiquitination | Uniprot | |
| K570 | Ubiquitination | Uniprot | |
| K579 | Ubiquitination | Uniprot | |
| S599 | Phosphorylation | Uniprot | |
| S611 | Phosphorylation | Uniprot | |
| T617 | Phosphorylation | Uniprot | |
| K635 | Ubiquitination | Uniprot | |
| Y651 | Phosphorylation | Uniprot | |
| K655 | Ubiquitination | Uniprot | |
| S668 | Phosphorylation | Uniprot | |
| S672 | Phosphorylation | Uniprot | |
| T674 | Phosphorylation | Uniprot | |
| S681 | Phosphorylation | Uniprot | |
| K688 | Ubiquitination | Uniprot | |
| S704 | Phosphorylation | Uniprot | |
| Y705 | Phosphorylation | Uniprot | |
| Y708 | Phosphorylation | Uniprot | |
| S711 | Phosphorylation | Uniprot | |
| T713 | Phosphorylation | Uniprot | |
| T722 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T725 | Phosphorylation | Uniprot | |
| S728 | Phosphorylation | Q13315 (ATM) | Uniprot |
| K732 | Sumoylation | Uniprot | |
| K732 | Ubiquitination | Uniprot | |
| S734 | Phosphorylation | Uniprot | |
| K736 | Sumoylation | Uniprot | |
| K736 | Ubiquitination | Uniprot | |
| S740 | Phosphorylation | Uniprot | |
| S742 | Phosphorylation | Uniprot | |
| R743 | Methylation | Uniprot | |
| K748 | Ubiquitination | Uniprot |
Research Backgrounds
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.
Acetylated by MCM3AP.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
Nucleus.
Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (Probable). Associated with the replication-specific DNA polymerase alpha. Interacts with MCMBP. Interacts with ANKRD17. Interacts with MCM3AP isoform MCM3AP; this interaction leads to MCM3 acetylation.
Belongs to the MCM family.
Research Fields
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Genetic Information Processing > Replication and repair > DNA replication.
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