ULK1 Mouse Monoclonal Antibody | Affinity Biosciences

IF/ICC

1/1

Product:	ULK1 Mouse Monoclonal Antibody
Catalog:	BF8364
Description:	Mouse monoclonal antibody to ULK1
Application:	IF/ICC
Reactivity:	Human
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	120~150 kDa; 113kD(Calculated).
Uniprot:	O75385

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Monoclonal [AFfirm8364]

Specificity:

ULK1 Mouse Monoclonal Antibody detects endogenous levels of total ULK1

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ATG 1; ATG1; ATG1 autophagy related 1 homolog; ATG1A; Autophagy related protein 1 homolog; Autophagy-related protein 1 homolog; FLJ38455; FLJ46475; hATG1; KIAA0722; Serine/threonine protein kinase ULK1; Serine/threonine protein kinase Unc51.1; Serine/threonine-protein kinase ULK1; ULK 1; ULK1; ULK1_HUMAN; Unc 51 (C. elegans) like kinase 1; UNC 51; Unc 51 like kinase 1; Unc-51 like kinase 1 (C. elegans); Unc-51-like kinase 1; UNC51; UNC51, C. elegans, homolog of; Unc51.1;

Immunogens

Immunogen:

Uniprot:

O75385(ULK1_HUMAN) >>Visit HPA database.

Gene(ID):

ULK1(8408)

Expression:

O75385 ULK1_HUMAN:

Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.

Sequence:

O75385 ULK1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEPGRGGTETVGKFEFSRKDLIGHGAFAVVFKGRHREKHDLEVAVKCINKKNLAKSQTLLGKEIKILKELKHENIVALYDFQEMANSVYLVMEYCNGGDLADYLHAMRTLSEDTIRLFLQQIAGAMRLLHSKGIIHRDLKPQNILLSNPAGRRANPNSIRVKIADFGFARYLQSNMMAATLCGSPMYMAPEVIMSQHYDGKADLWSIGTIVYQCLTGKAPFQASSPQDLRLFYEKNKTLVPTIPRETSAPLRQLLLALLQRNHKDRMDFDEFFHHPFLDASPSVRKSPPVPVPSYPSSGSGSSSSSSSTSHLASPPSLGEMQQLQKTLASPADTAGFLHSSRDSGGSKDSSCDTDDFVMVPAQFPGDLVAEAPSAKPPPDSLMCSGSSLVASAGLESHGRTPSPSPPCSSSPSPSGRAGPFSSSRCGASVPIPVPTQVQNYQRIERNLQSPTQFQTPRSSAIRRSGSTSPLGFARASPSPPAHAEHGGVLARKMSLGGGRPYTPSPQVGTIPERPGWSGTPSPQGAEMRGGRSPRPGSSAPEHSPRTSGLGCRLHSAPNLSDLHVVRPKLPKPPTDPLGAVFSPPQASPPQPSHGLQSCRNLRGSPKLPDFLQRNPLPPILGSPTKAVPSFDFPKTPSSQNLLALLARQGVVMTPPRNRTLPDLSEVGPFHGQPLGPGLRPGEDPKGPFGRSFSTSRLTDLLLKAAFGTQAPDPGSTESLQEKPMEIAPSAGFGGSLHPGARAGGTSSPSPVVFTVGSPPSGSTPPQGPRTRMFSAGPTGSASSSARHLVPGPCSEAPAPELPAPGHGCSFADPITANLEGAVTFEAPDLPEETLMEQEHTEILRGLRFTLLFVQHVLEIAALKGSASEAAGGPEYQLQESVVADQISLLSREWGFAEQLVLYLKVAELLSSGLQSAIDQIRAGKLCLSSTVKQVVRRLNELYKASVVSCQGLSLRLQRFFLDKQRLLDRIHSITAERLIFSHAVQMVQSAALDEMFQHREGCVPRYHKALLLLEGLQHMLSDQADIENVTKCKLCIERRLSALLTGICA

PTMs - O75385 As Substrate

O75385

Site	PTM Type	Enzyme	Source
K13	Ubiquitination		Uniprot
K46	Ubiquitination		Uniprot
K55	Ubiquitination		Uniprot
K62	Ubiquitination		Uniprot
S111	Phosphorylation		Uniprot
S131	Phosphorylation		Uniprot
K140	Ubiquitination		Uniprot
S158	Phosphorylation		Uniprot
K162	Acetylation		Uniprot
K162	Ubiquitination		Uniprot
T180	Phosphorylation	O75385 (ULK1)	Uniprot
S225	Phosphorylation		Uniprot
K235	Ubiquitination		Uniprot
K237	Ubiquitination		Uniprot
S281	Phosphorylation		Uniprot
S317	Phosphorylation	Q13131 (PRKAA1) , P54646 (PRKAA2)	Uniprot
S330	Phosphorylation		Uniprot
S403	Phosphorylation		Uniprot
S405	Phosphorylation		Uniprot
S409	Phosphorylation		Uniprot
S415	Phosphorylation		Uniprot
S429	Phosphorylation		Uniprot
S450	Phosphorylation		Uniprot
T456	Phosphorylation		Uniprot
S459	Phosphorylation		Uniprot
S460	Phosphorylation		Uniprot
S465	Phosphorylation		Uniprot
S467	Phosphorylation		Uniprot
T468	Phosphorylation		Uniprot
S469	Phosphorylation		Uniprot
S477	Phosphorylation		Uniprot
S479	Phosphorylation		Uniprot
S495	Phosphorylation		Uniprot
S522	Phosphorylation		Uniprot
R529	Methylation		Uniprot
S533	Phosphorylation		Uniprot
S538	Phosphorylation		Uniprot
S539	Phosphorylation		Uniprot
S544	Phosphorylation		Uniprot
S556	Phosphorylation	P54646 (PRKAA2)	Uniprot
S561	Phosphorylation		Uniprot
T575	Phosphorylation		Uniprot
S583	Phosphorylation		Uniprot
S588	Phosphorylation		Uniprot
S593	Phosphorylation		Uniprot
S598	Phosphorylation		Uniprot
S605	Phosphorylation		Uniprot
K607	Acetylation		Uniprot
S623	Phosphorylation		Uniprot
T625	Phosphorylation		Uniprot
S630	Phosphorylation		Uniprot
T636	Phosphorylation		Uniprot
S638	Phosphorylation	P42345 (MTOR) , P54646 (PRKAA2) , Q13131 (PRKAA1)	Uniprot
S639	Phosphorylation		Uniprot
T654	Phosphorylation		Uniprot
T660	Phosphorylation		Uniprot
S665	Phosphorylation		Uniprot
S694	Phosphorylation		Uniprot
T695	Phosphorylation		Uniprot
T699	Phosphorylation		Uniprot
T709	Phosphorylation		Uniprot
S716	Phosphorylation		Uniprot
T717	Phosphorylation		Uniprot
S719	Phosphorylation		Uniprot
S748	Phosphorylation		Uniprot
S758	Phosphorylation	P42345 (MTOR)	Uniprot
S761	Phosphorylation		Uniprot
T764	Phosphorylation		Uniprot
S775	Phosphorylation		Uniprot
S781	Phosphorylation		Uniprot
S949	Phosphorylation		Uniprot
S954	Phosphorylation		Uniprot
S1042	Phosphorylation		Uniprot
T1046	Phosphorylation		Uniprot

PTMs - O75385 As Enzyme

O75385

Substrate	Site	Source
A2RUS2 (DENND3)	S472	Uniprot
A2RUS2 (DENND3)	S490	Uniprot
O00560 (SDCBP)	S6	Uniprot
O75143 (ATG13)	S355	Uniprot
O75385 (ULK1)	T180	Uniprot
Q13131 (PRKAA1)	S360	Uniprot
Q13131 (PRKAA1)	T368	Uniprot
Q13131 (PRKAA1)	S397	Uniprot
Q13131 (PRKAA1)	S486	Uniprot
Q13131 (PRKAA1)	T488	Uniprot
Q7Z3C6 (ATG9A)	S761	Uniprot
Q86WV6 (TMEM173)	S366	Uniprot
Q8N122 (RPTOR)	S792	Uniprot
Q8N122 (RPTOR)	S855	Uniprot
Q8N122 (RPTOR)	S859	Uniprot

Research Backgrounds

O75385_ULK1_HUMAN

Function:

Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy.

PTMs:

Autophosphorylated. Phosphorylated under nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. Under nutrient sufficiency, phosphorylated by MTOR/mTOR, disrupting the interaction with AMPK and preventing activation of ULK1 (By similarity). In response to nutrient limitation, phosphorylated and activated by AMPK, leading to activate autophagy.

Acetylated by KAT5/TIP60 under autophagy induction, promoting protein kinase activity.

Subcellular Location:

Cytoplasm>Cytosol. Preautophagosomal structure.
Note: Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome.

Tissue Specificity:

Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.

Subunit Structure:

Interacts with GABARAP and GABARAPL2. Interacts (via C-terminus) with ATG13. Part of a complex consisting of ATG13, ATG101, ULK1 and RB1CC1. Associates with the mammalian target of rapamycin complex 1 (mTORC1) through an interaction with RPTOR; the association depends on nutrient conditions and is reduced during starvation. Interacts with FEZ1; SCOC interferes with FEZ1-binding. Interacts with TBC1D14. Interacts (phosphorylated form) with TRIM5. When phosphorylated at Ser-317, interacts with MEFV and BECN1 simultaneously. Interacts with TRIM21 and IRF3, in the presence of TRIM21. Interacts with SESN2. Interacts with SQSTM1. Interacts with C9orf72. Interacts with WDR45.

Family&Domains:

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)

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AFfirm™ ULK1 Mouse Monoclonal Antibody - #BF8364