AFfirm™ FOXO4 Mouse Monoclonal Antibody - #BF8327
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Fold/Unfold
AFX; AFX1; Afxh; ALL1-fused gene from X chromosome; Fork head domain transcription factor AFX1; Forkhead box O4; Forkhead box protein O4; FOXO 4; Foxo4; FOXO4_HUMAN; MGC117660; MGC120490; Mixed lineage leukemia, translocated to, 7; MLLT7; Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog, Drosophila); translocated to, 7; Myeloid/lymphoid or mixed lineage leukemia, translocated to, 7; RGD1561201;
Immunogens
Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.
- P98177 FOXO4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDPGNENSATEAAAIIDLDPDFEPQSRPRSCTWPLPRPEIANQPSEPPEVEPDLGEKVHTEGRSEPILLPSRLPEPAGGPQPGILGAVTGPRKGGSRRNAWGNQSYAELISQAIESAPEKRLTLAQIYEWMVRTVPYFKDKGDSNSSAGWKNSIRHNLSLHSKFIKVHNEATGKSSWWMLNPEGGKSGKAPRRRAASMDSSSKLLRGRSKAPKKKPSVLPAPPEGATPTSPVGHFAKWSGSPCSRNREEADMWTTFRPRSSSNASSVSTRLSPLRPESEVLAEEIPASVSSYAGGVPPTLNEGLELLDGLNLTSSHSLLSRSGLSGFSLQHPGVTGPLHTYSSSLFSPAEGPLSAGEGCFSSSQALEALLTSDTPPPPADVLMTQVDPILSQAPTLLLLGGLPSSSKLATGVGLCPKPLEAPGPSSLVPTLSMIAPPPVMASAPIPKALGTPVLTPPTEAASQDRMPQDLDLDMYMENLECDMDNIISDLMDEGEGLDFNFEPDP
PTMs - P98177 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Ubiquitination | Uniprot | ||
| S26 | Phosphorylation | Uniprot | |
| S30 | Phosphorylation | Uniprot | |
| T32 | Phosphorylation | P31749 (AKT1) , P31751 (AKT2) | Uniprot |
| S111 | Phosphorylation | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| S159 | Phosphorylation | Uniprot | |
| K186 | Acetylation | Uniprot | |
| K189 | Acetylation | Uniprot | |
| S197 | Phosphorylation | P31749 (AKT1) | Uniprot |
| S200 | Phosphorylation | Uniprot | |
| T227 | Phosphorylation | P45983 (MAPK8) | Uniprot |
| S230 | Phosphorylation | P45983 (MAPK8) | Uniprot |
| S241 | Phosphorylation | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| S261 | Phosphorylation | Uniprot | |
| S262 | Phosphorylation | P31749 (AKT1) | Uniprot |
| S265 | Phosphorylation | Uniprot | |
| S268 | Phosphorylation | Uniprot | |
| S272 | Phosphorylation | Uniprot | |
| S278 | Phosphorylation | Uniprot | |
| S288 | Phosphorylation | Uniprot | |
| Y292 | Phosphorylation | Uniprot | |
| K407 | Acetylation | Uniprot | |
| T451 | Phosphorylation | P45983 (MAPK8) , P45984 (MAPK9) | Uniprot |
| T455 | Phosphorylation | P45984 (MAPK9) , P45983 (MAPK8) | Uniprot |
| T458 | Phosphorylation | Uniprot |
Research Backgrounds
Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity.
Acetylation by CREBBP/CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity.
Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization. May be phosphorylated at multiple sites by NLK.
Monoubiquitinated; monoubiquitination is induced by oxidative stress and reduced by deacetylase inhibitors; results in its relocalization to the nucleus and its increased transcriptional activity. Deubiquitinated by USP7; deubiquitination is induced by oxidative stress; enhances its interaction with USP7 and consequently, deubiquitination; increases its translocation to the cytoplasm and inhibits its transcriptional activity. Hydrogene-peroxide-induced ubiquitination and USP7-mediated deubiquitination have no major effect on its protein stability.
Cytoplasm. Nucleus.
Note: When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm.
Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.
Interacts with CREBBP/CBP, CTNNB1, MYOCD, SIRT1, SRF and YWHAZ. Acetylated by CREBBP/CBP and deacetylated by SIRT1. Binding of YWHAZ inhibits DNA-binding. Interacts with USP7; the interaction is enhanced in presence of hydrogen peroxide and occurs independently of TP53. Interacts with NLK, and this inhibits monoubiquitination and transcriptional activity. Interacts with FOXK1; the interaction inhibits MEF2C transactivation activity (By similarity).
Research Fields
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)
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