TRIM32 Mouse Monoclonal Antibody | Affinity Biosciences

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Western blot analysis of extracts from 293 cells(serum starvation treatment), using TRIM32 Mouse Monoclonal Antibody at 1/1000 dilution.

Product:	TRIM32 Mouse Monoclonal Antibody
Catalog:	BF8323
Description:	Mouse monoclonal antibody to TRIM32
Application:	WB
Reactivity:	Human
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	72kDa; 72kD(Calculated).
Uniprot:	Q13049

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
50ul	$250	In stock
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Monoclonal [AFfirm8323]

Specificity:

TRIM32 Mouse Monoclonal Antibody detects endogenous levels of total TRIM32

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

72 kda Tat interacting Protein; 72 kDa Tat-interacting protein; BBS11; E3 ubiquitin-protein ligase TRIM32; HT2A; LGMD2H; Limb girdle muscular dystrophy 2H (autosomal recessive); Limb girdle muscular dystrophy 2H; Muscular dystrophy Hutterite type; TAT interactive protein 72KD; TATIP; TRI32_HUMAN; Trim32; Tripartite Motif Containing Protein 32; Tripartite motif-containing protein 32; Zinc finger protein HT2A;

Immunogens

Immunogen:

Uniprot:

Q13049(TRI32_HUMAN) >>Visit HPA database.

Gene(ID):

TRIM32(22954)

Expression:

Q13049 TRI32_HUMAN:

Spleen, thymus, prostate, testis, ovary, intestine, colon and skeletal muscle.

Sequence:

Q13049 TRI32_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAAAASHLNLDALREVLECPICMESFTEEQLRPKLLHCGHTICRQCLEKLLASSINGVRCPFCSKITRITSLTQLTDNLTVLKIIDTAGLSEAVGLLMCRSCGRRLPRQFCRSCGLVLCEPCREADHQPPGHCTLPVKEAAEERRRDFGEKLTRLRELMGELQRRKAALEGVSKDLQARYKAVLQEYGHEERRVQDELARSRKFFTGSLAEVEKSNSQVVEEQSYLLNIAEVQAVSRCDYFLAKIKQADVALLEETADEEEPELTASLPRELTLQDVELLKVGHVGPLQIGQAVKKPRTVNVEDSWAMEATASAASTSVTFREMDMSPEEVVASPRASPAKQRGPEAASNIQQCLFLKKMGAKGSTPGMFNLPVSLYVTSQGEVLVADRGNYRIQVFTRKGFLKEIRRSPSGIDSFVLSFLGADLPNLTPLSVAMNCQGLIGVTDSYDNSLKVYTLDGHCVACHRSQLSKPWGITALPSGQFVVTDVEGGKLWCFTVDRGSGVVKYSCLCSAVRPKFVTCDAEGTVYFTQGLGLNLENRQNEHHLEGGFSIGSVGPDGQLGRQISHFFSENEDFRCIAGMCVDARGDLIVADSSRKEILHFPKGGGYSVLIREGLTCPVGIALTPKGQLLVLDCWDHCIKIYSYHLRRYSTP

PTMs - Q13049 As Substrate

Q13049

Site	PTM Type	Source
S7	Phosphorylation	Uniprot
K50	Ubiquitination	Uniprot
K66	Ubiquitination	Uniprot
S72	Phosphorylation	Uniprot
K139	Ubiquitination	Uniprot
K152	Ubiquitination	Uniprot
K167	Ubiquitination	Uniprot
K175	Ubiquitination	Uniprot
K182	Ubiquitination	Uniprot
K204	Ubiquitination	Uniprot
K215	Ubiquitination	Uniprot
Y241	Phosphorylation	Uniprot
K245	Ubiquitination	Uniprot
K247	Ubiquitination	Uniprot
T274	Phosphorylation	Uniprot
K282	Ubiquitination	Uniprot
S306	Phosphorylation	Uniprot
T321	Phosphorylation	Uniprot
S328	Phosphorylation	Uniprot
S335	Phosphorylation	Uniprot
S339	Phosphorylation	Uniprot
K342	Ubiquitination	Uniprot
K359	Ubiquitination	Uniprot
K401	Ubiquitination	Uniprot
K405	Ubiquitination	Uniprot
K471	Ubiquitination	Uniprot
K506	Ubiquitination	Uniprot
S570	Phosphorylation	Uniprot
K604	Ubiquitination	Uniprot
T617	Phosphorylation	Uniprot
K627	Ubiquitination	Uniprot
S651	Phosphorylation	Uniprot

Research Backgrounds

Q13049_TRI32_HUMAN

Function:

Has an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation. May ubiquitinate BBS2. May play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo. Binds specifically to the activation domain of HIV-1 Tat and can also interact with the HIV-2 and EIAV Tat proteins in vivo.

PTMs:

Ubiquitinated.

Subcellular Location:

Cytoplasm.
Note: Localized in cytoplasmic bodies, often located around the nucleus.

Tissue Specificity:

Spleen, thymus, prostate, testis, ovary, intestine, colon and skeletal muscle.

Subunit Structure:

Interacts with DTNBP1. It self-associates.

Family&Domains:

Belongs to the TRIM/RBCC family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)

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AFfirm™ TRIM32 Mouse Monoclonal Antibody - #BF8323