FEN1 Mouse Monoclonal Antibody | Affinity Biosciences

Product:	FEN1 Mouse Monoclonal Antibody
Catalog:	BF8322
Description:	Mouse monoclonal antibody to FEN1
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	43kDa; 43kD(Calculated).
Uniprot:	P39748

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Monoclonal [AFfirm8322]

Specificity:

FEN1 Mouse Monoclonal Antibody detects endogenous levels of total FEN1

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DNase IV; FEN-1; FEN1; FEN1_HUMAN; Flap endonuclease 1; Flap structure specific endonuclease 1; Flap structure-specific endonuclease 1; hFEN-1; hFEN1; Maturation factor 1; MF1; Rad2;

Immunogens

Immunogen:

Uniprot:

P39748(FEN1_HUMAN) >>Visit HPA database.

Gene(ID):

FEN1(2237)

Description:

Flap endonuclease-1 (FEN-1) is a structure-specific nuclease with multiple functions in DNA processing pathways (1,2). The replication and DNA repair activities of FEN-1 are critical for genomic stability in the eukaryotic cell. Through interaction with proliferation cell nuclear antigen (PCNA), FEN-1 helps coordinate Okazaki fragment maturation by removing RNA-DNA primers (3). FEN-1 is also required for non-homologous end joining of double stranded DNA breaks in long patch base excision repair (4,5). The multi-functional activities of FEN-1 are regulated by various mechanisms, including protein partner interactions (6,7), post-translational modifications (8,9), and subcellular re-localization in response to cell cycle or DNA damage (10).

Sequence:

P39748 FEN1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGIQGLAKLIADVAPSAIRENDIKSYFGRKVAIDASMSIYQFLIAVRQGGDVLQNEEGETTSHLMGMFYRTIRMMENGIKPVYVFDGKPPQLKSGELAKRSERRAEAEKQLQQAQAAGAEQEVEKFTKRLVKVTKQHNDECKHLLSLMGIPYLDAPSEAEASCAALVKAGKVYAAATEDMDCLTFGSPVLMRHLTASEAKKLPIQEFHLSRILQELGLNQEQFVDLCILLGSDYCESIRGIGPKRAVDLIQKHKSIEEIVRRLDPNKYPVPENWLHKEAHQLFLEPEVLDPESVELKWSEPNEEELIKFMCGEKQFSEERIRSGVKRLSKSRQGSTQGRLDDFFKVTGSLSSAKRKEPEPKGSTKKKAKTGAAGKFKRGK

PTMs - P39748 As Substrate

P39748

Site	PTM Type	Enzyme	Source
K8	Ubiquitination		Uniprot
S16	Phosphorylation		Uniprot
R19	Methylation		Uniprot
K24	Sumoylation		Uniprot
K24	Ubiquitination		Uniprot
K80	Acetylation		Uniprot
K80	Ubiquitination		Uniprot
K88	Sumoylation		Uniprot
K88	Ubiquitination		Uniprot
K93	Sumoylation		Uniprot
K99	Ubiquitination		Uniprot
R100	Methylation		Uniprot
S101	Phosphorylation		Uniprot
R104	Methylation		Uniprot
K109	Acetylation		Uniprot
K109	Ubiquitination		Uniprot
K125	Acetylation		Uniprot
K125	Sumoylation		Uniprot
K125	Ubiquitination		Uniprot
K128	Sumoylation		Uniprot
K128	Ubiquitination		Uniprot
K132	Ubiquitination		Uniprot
K135	Ubiquitination		Uniprot
K142	Ubiquitination		Uniprot
C163	S-Nitrosylation		Uniprot
K168	Sumoylation		Uniprot
K168	Ubiquitination		Uniprot
K171	Ubiquitination		Uniprot
S187	Phosphorylation	P06493 (CDK1)	Uniprot
R192	Methylation		Uniprot
T195	Phosphorylation	O14757 (CHEK1)	Uniprot
S197	Phosphorylation		Uniprot
K200	Acetylation		Uniprot
K200	Ubiquitination		Uniprot
K201	Acetylation		Uniprot
K201	Sumoylation		Uniprot
K201	Ubiquitination		Uniprot
Y234	Phosphorylation		Uniprot
K252	Ubiquitination		Uniprot
K254	Sumoylation		Uniprot
K254	Ubiquitination		Uniprot
S255	Phosphorylation		Uniprot
K267	Acetylation		Uniprot
K267	Sumoylation		Uniprot
K267	Ubiquitination		Uniprot
Y268	Phosphorylation		Uniprot
K277	Ubiquitination		Uniprot
S293	Phosphorylation		Uniprot
K297	Sumoylation		Uniprot
K297	Ubiquitination		Uniprot
K308	Sumoylation		Uniprot
K308	Ubiquitination		Uniprot
K314	Sumoylation		Uniprot
K314	Ubiquitination		Uniprot
S317	Phosphorylation		Uniprot
S329	Phosphorylation		Uniprot
S331	Phosphorylation		Uniprot
S335	Phosphorylation		Uniprot
T336	Phosphorylation		Uniprot
K345	Methylation		Uniprot
K345	Sumoylation		Uniprot
K345	Ubiquitination		Uniprot
S349	Phosphorylation		Uniprot
S351	Phosphorylation		Uniprot
S352	Phosphorylation		Uniprot
K354	Acetylation		Uniprot
K354	Sumoylation		Uniprot
K354	Ubiquitination		Uniprot
S363	Phosphorylation		Uniprot
T364	Phosphorylation		Uniprot
K375	Acetylation		Uniprot
K375	Sumoylation		Uniprot
K377	Acetylation		Uniprot
K380	Acetylation		Uniprot

Research Backgrounds

P39748_FEN1_HUMAN

Function:

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.

PTMs:

Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300.

Phosphorylation upon DNA damage induces relocalization to the nuclear plasma. Phosphorylation at Ser-187 by CDK2 occurs during late S-phase and results in dissociation from PCNA.

Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA.

Subcellular Location:

Nucleus>Nucleolus. Nucleus>Nucleoplasm.
Note: Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.

Mitochondrion.

Subunit Structure:

Three molecules of FEN1 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity. The C-terminal domain binds EP300; can bind simultaneously to both PCNA and EP300. Interacts with PCNA; can bind simultaneously to both PCNA and EP300. Interacts with DDX11; this interaction is direct and increases flap endonuclease activity of FEN1. Interacts with WDR4; regulating its endonuclease activity.

Family&Domains:

Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.

Research Fields

· Genetic Information Processing > Replication and repair > DNA replication.

· Genetic Information Processing > Replication and repair > Base excision repair.

· Genetic Information Processing > Replication and repair > Non-homologous end-joining.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

AFfirm™ FEN1 Mouse Monoclonal Antibody - #BF8322