AFfirm™ MLK3 Mouse Monoclonal Antibody - #BF8283
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*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Fold/Unfold
2610017K16Rik; EC 2.7.11.25; M3K11_HUMAN; Map3k11; MEKK11; MGC17114; Mitogen-activated protein kinase kinase kinase 11; Mixed lineage kinase 3; Mixed lineage protein kinase 3; Mlk-3; Mlk3; Protein tyrosine kinase PTK1; PTK1; RHOE; SH3 domain containing proline rich kinase; SPRK; Src-homology 3 domain-containing proline-rich kinase;
Immunogens
Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain.
- Q16584 M3K11_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEPLKSLFLKSPLGSWNGSGSGGGGGGGGGRPEGSPKAAGYANPVWTALFDYEPSGQDELALRKGDRVEVLSRDAAISGDEGWWAGQVGGQVGIFPSNYVSRGGGPPPCEVASFQELRLEEVIGIGGFGKVYRGSWRGELVAVKAARQDPDEDISVTAESVRQEARLFAMLAHPNIIALKAVCLEEPNLCLVMEYAAGGPLSRALAGRRVPPHVLVNWAVQIARGMHYLHCEALVPVIHRDLKSNNILLLQPIESDDMEHKTLKITDFGLAREWHKTTQMSAAGTYAWMAPEVIKASTFSKGSDVWSFGVLLWELLTGEVPYRGIDCLAVAYGVAVNKLTLPIPSTCPEPFAQLMADCWAQDPHRRPDFASILQQLEALEAQVLREMPRDSFHSMQEGWKREIQGLFDELRAKEKELLSREEELTRAAREQRSQAEQLRRREHLLAQWELEVFERELTLLLQQVDRERPHVRRRRGTFKRSKLRARDGGERISMPLDFKHRITVQASPGLDRRRNVFEVGPGDSPTFPRFRAIQLEPAEPGQAWGRQSPRRLEDSSNGERRACWAWGPSSPKPGEAQNGRRRSRMDEATWYLDSDDSSPLGSPSTPPALNGNPPRPSLEPEEPKRPVPAERGSSSGTPKLIQRALLRGTALLASLGLGRDLQPPGGPGRERGESPTTPPTPTPAPCPTEPPPSPLICFSLKTPDSPPTPAPLLLDLGIPVGQRSAKSPRREEEPRGGTVSPPPGTSRSAPGTPGTPRSPPLGLISRPRPSPLRSRIDPWSFVSAGPRPSPLPSPQPAPRRAPWTLFPDSDPFWDSPPANPFQGGPQDCRAQTKDMGAQAPWVPEAGP
PTMs - Q16584 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S11 | Phosphorylation | Uniprot | |
| S35 | Phosphorylation | Uniprot | |
| K261 | Ubiquitination | Uniprot | |
| K264 | Ubiquitination | Uniprot | |
| T277 | Phosphorylation | Q16584 (MAP3K11) | Uniprot |
| S281 | Phosphorylation | Q16584 (MAP3K11) , Q92918 (MAP4K1) | Uniprot |
| S394 | Phosphorylation | Uniprot | |
| T477 | Phosphorylation | Uniprot | |
| S493 | Phosphorylation | Uniprot | |
| K499 | Ubiquitination | Uniprot | |
| S507 | Phosphorylation | Uniprot | |
| R512 | Methylation | Uniprot | |
| S524 | Phosphorylation | Uniprot | |
| T526 | Phosphorylation | Uniprot | |
| S548 | Phosphorylation | Uniprot | |
| S555 | Phosphorylation | Uniprot | |
| S556 | Phosphorylation | Uniprot | |
| S570 | Phosphorylation | Uniprot | |
| K624 | Ubiquitination | Uniprot | |
| S654 | Phosphorylation | Uniprot | |
| S674 | Phosphorylation | P31749 (AKT1) | Uniprot |
| T677 | Phosphorylation | Uniprot | |
| S693 | Phosphorylation | Uniprot | |
| T702 | Phosphorylation | Uniprot | |
| S705 | Phosphorylation | Uniprot | |
| T708 | Phosphorylation | Uniprot | |
| S724 | Phosphorylation | Uniprot | |
| S727 | Phosphorylation | Uniprot | |
| T738 | Phosphorylation | Uniprot | |
| S740 | Phosphorylation | Uniprot | |
| S746 | Phosphorylation | Uniprot | |
| S748 | Phosphorylation | Uniprot | |
| T752 | Phosphorylation | Uniprot | |
| T755 | Phosphorylation | Uniprot | |
| S758 | Phosphorylation | Uniprot | |
| S770 | Phosphorylation | Uniprot | |
| S774 | Phosphorylation | Uniprot | |
| S783 | Phosphorylation | Uniprot | |
| S789 | Phosphorylation | P49840 (GSK3A) | Uniprot |
| S793 | Phosphorylation | P49840 (GSK3A) | Uniprot |
| S815 | Phosphorylation | Uniprot |
PTMs - Q16584 As Enzyme
Research Backgrounds
Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7. Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.
Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-277 is likely to be the main autophosphorylation site. Phosphorylation of Ser-555 and Ser-556 is induced by CDC42.
Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Location is cell cycle dependent.
Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain.
Homodimer; undergoes dimerization during activation. Interacts with MAP2K4/MKK4 (By similarity). Interacts with MAP2K7/MKK7. Found in a complex with SH3RF1, RAC1, MAP2K7/MKK7, MAPK8IP1/JIP1 and MAPK8/JNK1 (By similarity).
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Research Fields
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).
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