AFfirm™ NRF1 Mouse Monoclonal Antibody - #BF8271

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Product: NRF1 Mouse Monoclonal Antibody
Catalog: BF8271
Description: Mouse monoclonal antibody to NRF1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 55kD, 70kD; 54kD(Calculated).
Uniprot: Q16656

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   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
WB 1:500-1:3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Monoclonal [AFfirm8271]
Specificity:
NRF1 Mouse Monoclonal Antibody detects endogenous levels of total NRF1
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

alpha pal; alpha palindromic binding protein; Alpha palindromic-binding protein; Alpha-pal; locus control region factor 1; NFE2 related factor 1; NRF-1; Nrf1; NRF1_HUMAN; Nuclear respiratory factor 1;

Immunogens

Immunogen:
Uniprot:

Q16656(NRF1_HUMAN) >>Visit HPA database.

Gene(ID):
NRF1(4899)
Expression:
Q16656 NRF1_HUMAN:

Ubiquitously expressed with strongest expression in skeletal muscle.

Description:
Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.
Sequence:
MEEHGVTQTEHMATIEAHAVAQQVQQVHVATYTEHSMLSADEDSPSSPEDTSYDDSDILNSTAADEVTAHLAAAGPVGMAAAAAVATGKKRKRPHVFESNPSIRKRQQTRLLRKLRATLDEYTTRVGQQAIVLCISPSKPNPVFKVFGAAPLENVVRKYKSMILEDLESALAEHAPAPQEVNSELPPLTIDGIPVSVDKMTQAQLRAFIPEMLKYSTGRGKPGWGKESCKPIWWPEDIPWANVRSDVRTEEQKQRVSWTQALRTIVKNCYKQHGREDLLYAFEDQQTQTQATATHSIAHLVPSQTVVQTFSNPDGTVSLIQVGTGATVATLADASELPTTVTVAQVNYSAVADGEVEQNWATLQGGEMTIQTTQASEATQAVASLAEAAVAASQEMQQGATVTMALNSEAAAHAVATLAEATLQGGGQIVLSGETAAAVGALTGVQDANGLVQIPVSMYQTVVTSLAQGNGPVQVAMAPVTTRISDSAVTMDGQAVEVVTLEQ

PTMs - Q16656 As Substrate

Site PTM Type Enzyme
S39 Phosphorylation
S44 Phosphorylation
S46 Phosphorylation
S47 Phosphorylation
S52 Phosphorylation
T87 Phosphorylation
K92 Ubiquitination
S99 Phosphorylation
T109 Phosphorylation
T118 Phosphorylation
Y122 Phosphorylation
T123 Phosphorylation
S136 Phosphorylation
S138 Phosphorylation
K139 Ubiquitination
K214 Ubiquitination
K221 Acetylation
K226 Acetylation
T259 Phosphorylation
K267 Ubiquitination

Research Backgrounds

Function:

Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.

PTMs:

Phosphorylation enhances DNA binding.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed with strongest expression in skeletal muscle.

Subunit Structure:

Homodimer. Binds DNA as a dimer. Interacts with PPRC1.

Family&Domains:

Belongs to the NRF1/Ewg family.

Research Fields

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

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