AFfirm™ eIF2B epsilon Mouse Monoclonal Antibody - #BF8263

(7)  
Product: eIF2B epsilon Mouse Monoclonal Antibody
Catalog: BF8263
Description: Mouse monoclonal antibody to eIF2B epsilon
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Dog
Mol.Wt.: 80KD; 80kD(Calculated).
Uniprot: Q13144

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Monoclonal [AFfirm8263]
Specificity:
eIF2B epsilon Mouse Monoclonal Antibody detects endogenous levels of total eIF2B epsilon
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CACH; CLE; EI2BE_HUMAN; EIF 2B; eIF 2B GDP GTP exchange factor; eIF 2B GDP GTP exchange factor subunit epsilon; eIF-2B GDP-GTP exchange factor subunit epsilon; EIF2B; EIF2B5; EIF2BE; EIF2Bepsilon; Eukaryotic Translation Initiation Factor 2 Beta Epsilon; Eukaryotic translation initiation factor 2B subunit 5 epsilon 82kDa; Eukaryotic translation initiation factor 2B subunit 5 epsilon; LVWM; Translation initiation factor eIF 2B subunit epsilon; Translation initiation factor eIF-2B subunit epsilon;

Immunogens

Immunogen:
Uniprot:

Q13144(EI2BE_HUMAN) >>Visit HPA database.

Gene(ID):
EIF2B5(8893)
Sequence:
MAAPVVAPPGVVVSRANKRSGAGPGGSGGGGARGAEEEPPPPLQAVLVADSFDRRFFPISKDQPRVLLPLANVALIDYTLEFLTATGVQETFVFCCWKAAQIKEHLLKSKWCRPTSLNVVRIITSELYRSLGDVLRDVDAKALVRSDFLLVYGDVISNINITRALEEHRLRRKLEKNVSVMTMIFKESSPSHPTRCHEDNVVVAVDSTTNRVLHFQKTQGLRRFAFPLSLFQGSSDGVEVRYDLLDCHISICSPQVAQLFTDNFDYQTRDDFVRGLLVNEEILGNQIHMHVTAKEYGARVSNLHMYSAVCADVIRRWVYPLTPEANFTDSTTQSCTHSRHNIYRGPEVSLGHGSILEENVLLGSGTVIGSNCFITNSVIGPGCHIGDNVVLDQTYLWQGVRVAAGAQIHQSLLCDNAEVKERVTLKPRSVLTSQVVVGPNITLPEGSVISLHPPDAEEDEDDGEFSDDSGADQEKDKVKMKGYNPAEVGAAGKGYLWKAAGMNMEEEEELQQNLWGLKINMEEESESESEQSMDSEEPDSRGGSPQMDDIKVFQNEVLGTLQRGKEENISCDNLVLEINSLKYAYNISLKEVMQVLSHVVLEFPLQQMDSPLDSSRYCALLLPLLKAWSPVFRNYIKRAADHLEALAAIEDFFLEHEALGISMAKVLMAFYQLEILAEETILSWFSQRDTTDKGQQLRKNQQLQRFIQWLKEAEEESSEDD

PTMs - Q13144 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S20 Phosphorylation
S27 Phosphorylation
S51 Phosphorylation P48729 (CSNK1A1)
K61 Ubiquitination
K103 Ubiquitination
K108 Ubiquitination
K141 Ubiquitination
T182 Phosphorylation
K186 Ubiquitination
K217 Ubiquitination
Y319 Phosphorylation
K420 Ubiquitination
S447 Phosphorylation
S450 Phosphorylation
S466 Phosphorylation P48729 (CSNK1A1)
S469 Phosphorylation P48729 (CSNK1A1)
K481 Ubiquitination
Y483 Phosphorylation
K493 Ubiquitination
S525 Phosphorylation
S527 Phosphorylation
S529 Phosphorylation
S532 Phosphorylation
S535 Phosphorylation P49841 (GSK3B)
S540 Phosphorylation P49841 (GSK3B) , P49840 (GSK3A)
S544 Phosphorylation P49840 (GSK3A) , P49841 (GSK3B)
K565 Ubiquitination
Y583 Phosphorylation
S610 Phosphorylation
C618 S-Nitrosylation
K693 Ubiquitination
S717 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2)
S718 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2)

Research Backgrounds

Function:

Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.

PTMs:

Phosphorylated at Ser-544 by DYRK2; this is required for subsequent phosphorylation by GSK3B (By similarity). Phosphorylated on serine and threonine residues by GSK3B; phosphorylation inhibits its function.

Polyubiquitinated, probably by NEDD4.

Subunit Structure:

Complex of five different subunits; alpha, beta, gamma, delta and epsilon. Interacts with RGS2.

Family&Domains:

Belongs to the eIF-2B gamma/epsilon subunits family.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.