AFfirm™ ZAK Mouse Monoclonal Antibody - #BF8261

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Product: ZAK Mouse Monoclonal Antibody
Catalog: BF8261
Description: Mouse monoclonal antibody to ZAK
Application: IHC
Reactivity: Human
Prediction: Pig, Bovine, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 91 KD; 91kD(Calculated).
Uniprot: Q9NYL2

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 100ul $140 280 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFfirm8261]
Specificity:
ZAK Mouse Monoclonal Antibody detects endogenous levels of total ZAK
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AZK; Cervical cancer suppressor gene 4 protein; HCCS 4; HCCS-4; HCCS4; Human cervical cancer suppressor gene 4 protein; Leucine zipper and sterile alpha motif containing kinase; Leucine zipper- and sterile alpha motif-containing kinase; Mitogen activated protein kinase kinase kinase MLT; Mitogen-activated protein kinase kinase kinase MLT; Mixed lineage kinase 7; Mixed lineage kinase related kinase; Mixed lineage kinase related kinase MRK beta; Mixed lineage kinase with a leucine zipper and a sterile alpha motif; Mixed lineage kinase-related kinase; MLK 7; MLK like mitogen activated protein triple kinase; MLK related kinase; MLK-like mitogen-activated protein triple kinase; MLK-related kinase; MLK7; Mlklak; MLT; MLTK_HUMAN; MRK; Sterile alpha motif and leucine zipper containing kinase AZK; Sterile alpha motif- and leucine zipper-containing kinase AZK; Zak;

Immunogens

Immunogen:
Uniprot:

Q9NYL2(M3K20_HUMAN) >>Visit HPA database.

Gene(ID):
MAP3K20(51776)
Expression:
Q9NYL2 M3K20_HUMAN:

Ubiquitously expressed. Isoform 2 is the predominant form in all tissues examined, except for liver, in which isoform 1 is more highly expressed.

Sequence:
MSSLGASFVQIKFDDLQFFENCGGGSFGSVYRAKWISQDKEVAVKKLLKIEKEAEILSVLSHRNIIQFYGVILEPPNYGIVTEYASLGSLYDYINSNRSEEMDMDHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVVIAADGVLKICDFGASRFHNHTTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLVVEKNERLTIPSSCPRSFAELLHQCWEADAKKRPSFKQIISILESMSNDTSLPDKCNSFLHNKAEWRCEIEATLERLKKLERDLSFKEQELKERERRLKMWEQKLTEQSNTPLLPSFEIGAWTEDDVYCWVQQLVRKGDSSAEMSVYASLFKENNITGKRLLLLEEEDLKDMGIVSKGHIIHFKSAIEKLTHDYINLFHFPPLIKDSGGEPEENEEKIVNLELVFGFHLKPGTGPQDCKWKMYMEMDGDEIAITYIKDVTFNTNLPDAEILKMTKPPFVMEKWIVGIAKSQTVECTVTYESDVRTPKSTKHVHSIQWSRTKPQDEVKAVQLAIQTLFTNSDGNPGSRSDSSADCQWLDTLRMRQIASNTSLQRSQSNPILGSPFFSHFDGQDSYAAAVRRPQVPIKYQQITPVNQSRSSSPTQYGLTKNFSSLHLNSRDSGFSSGNTDTSSERGRYSDRSRNKYGRGSISLNSSPRGRYSGKSQHSTPSRGRYPGKFYRVSQSALNPHQSPDFKRSPRDLHQPNTIPGMPLHPETDSRASEEDSKVSEGGWTKVEYRKKPHRPSPAKTNKERARGDHRGWRNF

PTMs - Q9NYL2 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
S3 Phosphorylation
S7 Phosphorylation
K34 Ubiquitination
K52 Ubiquitination
Y78 Phosphorylation
Y84 Phosphorylation
Y93 Phosphorylation
K116 Acetylation
Y120 Phosphorylation
K128 Ubiquitination
S136 Phosphorylation
S155 Phosphorylation
T161 Phosphorylation Q9NYL2 (MAP3K20)
T162 Phosphorylation Q9NYL2 (MAP3K20)
S165 Phosphorylation Q9NYL2 (MAP3K20)
K248 Ubiquitination
T267 Phosphorylation
S268 Phosphorylation
K272 Ubiquitination
S275 Phosphorylation
S302 Phosphorylation
K304 Ubiquitination
K309 Ubiquitination
K316 Ubiquitination
K354 Acetylation
S362 Phosphorylation
S366 Phosphorylation
K369 Acetylation
K376 Acetylation
K401 Ubiquitination
K538 Acetylation
K544 Acetylation
S557 Phosphorylation
S563 Phosphorylation
S565 Phosphorylation
S567 Phosphorylation
S568 Phosphorylation
T576 Phosphorylation
S584 Phosphorylation
T586 Phosphorylation
S591 Phosphorylation
S593 Phosphorylation
S599 Phosphorylation
S603 Phosphorylation
S610 Phosphorylation
T628 Phosphorylation
S633 Phosphorylation
S635 Phosphorylation
S636 Phosphorylation
S637 Phosphorylation
T639 Phosphorylation
S648 Phosphorylation
S649 Phosphorylation
S654 Phosphorylation
S660 Phosphorylation
S661 Phosphorylation
T666 Phosphorylation
S667 Phosphorylation
S668 Phosphorylation
R670 Methylation
Y673 Phosphorylation
S674 Phosphorylation
R683 Methylation
S685 Phosphorylation
S687 Phosphorylation
S690 Phosphorylation
S691 Phosphorylation
R693 Methylation
T704 Phosphorylation
R707 Methylation
R709 Methylation
K713 Methylation
S718 Phosphorylation
S720 Phosphorylation
S727 Phosphorylation
S733 Phosphorylation
S757 Phosphorylation
S761 Phosphorylation
S764 Phosphorylation
T769 Phosphorylation
K770 Ubiquitination
S781 Phosphorylation

PTMs - Q9NYL2 As Enzyme

Substrate Site Source
O96017 (CHEK2) T68 Uniprot
Q9NYL2 (MAP3K20) T161 Uniprot
Q9NYL2 (MAP3K20) T162 Uniprot
Q9NYL2 (MAP3K20) S165 Uniprot

Research Backgrounds

Function:

Stress-activated component of a protein kinase signal transduction cascade. Regulates the JNK and p38 pathways. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Pro-apoptotic. Role in regulation of S and G2 cell cycle checkpoint by direct phosphorylation of CHEK2. Involved in limb development.

Phosphorylates histone H3 at 'Ser-28'. May have role in neoplastic cell transformation and cancer development. Causes cell shrinkage and disruption of actin stress fibers.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Translocates to the nucleus upon ultraviolet B irradiation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed. Isoform 2 is the predominant form in all tissues examined, except for liver, in which isoform 1 is more highly expressed.

Subunit Structure:

Homodimer. Interacts with PKN1 and ZNF33A. Component of a signaling complex containing at least AKAP13, PKN1, MAPK14, MAP3K20 and MAP2K3. Within this complex, AKAP13 interacts directly with PKN1, which in turn recruits MAPK14, MAP2K3 and MAP3K20.

Family&Domains:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

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