AFfirm™ ADRB2 Mouse Monoclonal Antibody - #BF8223

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Product: ADRB2 Mouse Monoclonal Antibody
Catalog: BF8223
Description: Mouse monoclonal antibody to ADRB2
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Rabbit, Dog
Mol.Wt.: 47kDa; 46kD(Calculated).
Uniprot: P07550

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Monoclonal [AFfirm8223]
Specificity:
ADRB2 Antibody detects endogenous levels of total ADRB2.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ADRB2; ADRB2_HUMAN; ADRB2R; ADRBR; Adrenergic beta 2 receptor surface; Adrenoceptor beta 2 surface; B2AR; BAR; beta 2 adrenoceptor; Beta 2 adrenoreceptor; Beta-2 adrenergic receptor; Beta-2 adrenoceptor; Beta-2 adrenoreceptor; BETA2AR; Catecholamine receptor; OTTHUMP00000160386;

Immunogens

Immunogen:
Uniprot:

P07550(ADRB2_HUMAN) >>Visit HPA database.

Gene(ID):
ADRB2(154)
Description:
This gene encodes beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2.
Sequence:
MGQPGNGSAFLLAPNGSHAPDHDVTQERDEVWVVGMGIVMSLIVLAIVFGNVLVITAIAKFERLQTVTNYFITSLACADLVMGLAVVPFGAAHILMKMWTFGNFWCEFWTSIDVLCVTASIETLCVIAVDRYFAITSPFKYQSLLTKNKARVIILMVWIVSGLTSFLPIQMHWYRATHQEAINCYANETCCDFFTNQAYAIASSIVSFYVPLVIMVFVYSRVFQEAKRQLQKIDKSEGRFHVQNLSQVEQDGRTGHGLRRSSKFCLKEHKALKTLGIIMGTFTLCWLPFFIVNIVHVIQDNLIRKEVYILLNWIGYVNSGFNPLIYCRSPDFRIAFQELLCLRRSSLKAYGNGYSSNGNTGEQSGYHVEQEKENKLLCEDLPGTEDFVGHQGTVPSDNIDSQGRNCSTNDSLL

PTMs - P07550 As Substrate

Site PTM Type Enzyme
Phosphorylation
Ubiquitination
T68 Phosphorylation
Y132 Phosphorylation P08069 (IGF1R) , P06213 (INSR)
Y141 Phosphorylation P06213 (INSR) , P08069 (IGF1R)
Y219 Phosphorylation
S246 Phosphorylation
S261 Phosphorylation Q05655 (PRKCD)
S262 Phosphorylation Q05655 (PRKCD)
Y308 Phosphorylation
Y326 Phosphorylation
S345 Phosphorylation P31749 (AKT1) , P17612 (PRKACA) , Q05655 (PRKCD)
S346 Phosphorylation P17612 (PRKACA) , Q05655 (PRKCD) , P31749 (AKT1)
Y350 Phosphorylation P06213 (INSR)
Y354 Phosphorylation P06213 (INSR)
S355 Phosphorylation P43250 (GRK6) , P35626 (GRK3) , P25098 (GRK2) , P34947 (GRK5)
S356 Phosphorylation P43250 (GRK6) , P25098 (GRK2) , P35626 (GRK3) , P34947 (GRK5)
T360 Phosphorylation
S364 Phosphorylation
T384 Phosphorylation P25098 (GRK2) , P34947 (GRK5)
T393 Phosphorylation P34947 (GRK5)
S396 Phosphorylation P25098 (GRK2) , P34947 (GRK5)
S401 Phosphorylation P25098 (GRK2) , P34947 (GRK5)
S407 Phosphorylation P25098 (GRK2) , P34947 (GRK5)
T408 Phosphorylation
S411 Phosphorylation P25098 (GRK2) , P34947 (GRK5)

Research Backgrounds

Function:

Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.

PTMs:

Palmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation.

Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK.

Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the receptor.

Polyubiquitinated. Agonist-induced ubiquitination leads to sort internalized receptors to the lysosomes for degradation. Deubiquitination by USP20 and USP33, leads to ADRB2 recycling and resensitization after prolonged agonist stimulation. USP20 and USP33 are constitutively associated and are dissociated immediately after agonist stimulation. Ubiquitination by the VHL-E3 ligase complex is oxygen-dependent.

Hydroxylation by EGLN3 occurs only under normoxia and increases the interaction with VHL and the subsequent ubiquitination and degradation of ADRB2.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Early endosome.
Note: Colocalizes with VHL at the cell membrane (PubMed:19584355). Activated receptors are internalized into endosomes prior to their degradation in lysosomes (PubMed:20559325).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Binds SLC9A3R1 and GPRASP1. Interacts with ARRB1 and ARRB2. Interacts with SRC, USP20 and USP33. Interacts with VHL; the interaction, which is increased on hydroxylation of ADRB2, ubiquitinates ADRB2 leading to its degradation. Interacts with EGLN3; the interaction hydroxylates ADRB2 facilitating VHL-E3 ligase-mediated ubiquitination. Interacts (via PDZ-binding motif) with SNX27 (via PDZ domain); the interaction is required when endocytosed to prevent degradation in lysosomes and promote recycling to the plasma membrane. Interacts with CNIH4. Interacts with ARRDC3.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB2 sub-subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

· Organismal Systems > Endocrine system > Renin secretion.

· Organismal Systems > Digestive system > Salivary secretion.

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