ROCK2 Mouse Monoclonal Antibody | Affinity Biosciences

Product:	ROCK2 Mouse Monoclonal Antibody
Catalog:	BF8213
Description:	Mouse monoclonal antibody to ROCK2
Application:	WB
Reactivity:	Human, Mouse, Rat, Monkey
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	165kDa; 161kD(Calculated).
Uniprot:	O75116

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat,Monkey

Clonality:

Monoclonal [AFfirm8213]

Specificity:

ROCK2 Antibody detects endogenous levels of total ROCK2.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

coiled-coil-containing protein kinase 2; KIAA0619; p164 ROCK 2; p164 ROCK-2; Rho associated coiled coil containing protein kinase 2; Rho associated protein kinase 2; Rho associated, coiled coil containing protein kinase II; Rho kinase 2; Rho-associated; Rho-associated protein kinase 2; ROCK 2; Rock II; Rock2; ROCK2_HUMAN; Rock2m; ROK alpha; ROKalpha;

Immunogens

Immunogen:

Uniprot:

O75116(ROCK2_HUMAN) >>Visit HPA database.

Gene(ID):

ROCK2(9475)

Expression:

O75116 ROCK2_HUMAN:

Expressed in the brain (at protein level).

Description:

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq]

Sequence:

O75116 ROCK2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSRPPPTGKMPGAPETAPGDGAGASRQRKLEALIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGFYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNGVEEIRQHPFFKNDQWHWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRENLLLSDSPSCRETDSIQSRKNEESQEIQKKLYTLEEHLSNEMQAKEELEQKCKSVNTRLEKTAKELEEEITLRKSVESALRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQNSQISTEKVNQLQRQLDETNALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSALESERRDRTHGSEIINDLQGRICGLEEDLKNGKILLAKVELEKRQLQERFTDLEKEKSNMEIDMTYQLKVIQQSLEQEEAEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLLEERTLKQKVENLLLEAEKRCSLLDCDLKQSQQKINELLKQKDVLNEDVRNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLMEMKMNLEKQNAELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLGKELQQKKQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDATIASLEETNRTLTSDVANLANEKEELNNKLKDVQEQLSRLKDEEISAAAIKAQFEKQLLTERTLKTQAVNKLAEIMNRKEPVKRGNDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGLDSSSIGSGPGDAEADDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISTAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPS

PTMs - O75116 As Substrate

O75116

Site	PTM Type	Enzyme	Source
T16	Phosphorylation		Uniprot
S25	Phosphorylation		Uniprot
K29	Ubiquitination		Uniprot
Y92	Phosphorylation		Uniprot
K96	Ubiquitination		Uniprot
K121	Ubiquitination		Uniprot
K125	Ubiquitination		Uniprot
K216	Ubiquitination		Uniprot
T249	Phosphorylation		Uniprot
S298	Phosphorylation		Uniprot
K323	Ubiquitination		Uniprot
K355	Ubiquitination		Uniprot
K391	Ubiquitination		Uniprot
T396	Phosphorylation		Uniprot
T414	Phosphorylation	O75116 (ROCK2)	Uniprot
S423	Phosphorylation		Uniprot
S425	Phosphorylation		Uniprot
K448	Ubiquitination		Uniprot
K482	Ubiquitination		Uniprot
K492	Ubiquitination		Uniprot
K505	Ubiquitination		Uniprot
K511	Ubiquitination		Uniprot
K537	Ubiquitination		Uniprot
K556	Ubiquitination		Uniprot
T567	Phosphorylation		Uniprot
T573	Phosphorylation		Uniprot
S575	Phosphorylation		Uniprot
T577	Phosphorylation		Uniprot
K590	Ubiquitination		Uniprot
K605	Ubiquitination		Uniprot
K618	Ubiquitination		Uniprot
K669	Ubiquitination		Uniprot
K709	Ubiquitination		Uniprot
K720	Ubiquitination		Uniprot
Y722	Phosphorylation	P12931 (SRC)	Uniprot
S724	Phosphorylation		Uniprot
K729	Ubiquitination		Uniprot
K749	Ubiquitination		Uniprot
K759	Ubiquitination		Uniprot
K795	Ubiquitination		Uniprot
K860	Ubiquitination		Uniprot
K877	Ubiquitination		Uniprot
S930	Phosphorylation		Uniprot
Y936	Phosphorylation		Uniprot
S937	Phosphorylation		Uniprot
K946	Acetylation		Uniprot
K951	Ubiquitination		Uniprot
K964	Ubiquitination		Uniprot
T967	Phosphorylation	P53350 (PLK1)	Uniprot
K989	Ubiquitination		Uniprot
K995	Ubiquitination		Uniprot
K997	Ubiquitination		Uniprot
K1007	Ubiquitination		Uniprot
K1017	Ubiquitination		Uniprot
K1022	Ubiquitination		Uniprot
K1071	Ubiquitination		Uniprot
T1078	Phosphorylation		Uniprot
K1083	Ubiquitination		Uniprot
K1086	Ubiquitination		Uniprot
S1099	Phosphorylation	P53350 (PLK1)	Uniprot
S1114	Phosphorylation		Uniprot
S1121	Phosphorylation		Uniprot
S1132	Phosphorylation		Uniprot
S1133	Phosphorylation	P53350 (PLK1)	Uniprot
S1134	Phosphorylation		Uniprot
S1137	Phosphorylation		Uniprot
T1165	Phosphorylation		Uniprot
K1181	Ubiquitination		Uniprot
S1187	Phosphorylation		Uniprot
S1194	Phosphorylation		Uniprot
Y1197	Phosphorylation		Uniprot
T1212	Phosphorylation		Uniprot
K1222	Ubiquitination		Uniprot
Y1232	Phosphorylation		Uniprot
S1238	Phosphorylation		Uniprot
K1252	Ubiquitination		Uniprot
K1316	Ubiquitination		Uniprot
Y1318	Phosphorylation		Uniprot
Y1319	Phosphorylation		Uniprot
T1334	Phosphorylation		Uniprot
K1339	Ubiquitination		Uniprot
K1350	Acetylation		Uniprot
K1351	Acetylation		Uniprot
K1351	Ubiquitination		Uniprot
S1361	Phosphorylation		Uniprot
S1362	Phosphorylation		Uniprot
T1365	Phosphorylation		Uniprot
S1366	Phosphorylation		Uniprot
K1368	Methylation		Uniprot
K1368	Ubiquitination		Uniprot
S1374	Phosphorylation	P53350 (PLK1)	Uniprot
S1379	Phosphorylation		Uniprot
S1388	Phosphorylation		Uniprot

PTMs - O75116 As Enzyme

O75116

Substrate	Site	Source
O14974 (PPP1R12A)	T443	Uniprot
O14974 (PPP1R12A)	T696	Uniprot
O14974 (PPP1R12A)	S852	Uniprot
O14974-1 (PPP1R12A)	T853	Uniprot
O75116 (ROCK2)	T414	Uniprot
P05067-4 (APP)	T654	Uniprot
P08670 (VIM)	S39	Uniprot
P08670 (VIM)	S72	Uniprot
P15311 (EZR)	T567	Uniprot
P19634 (SLC9A1)	T653	Uniprot
P24844 (MYL9)	S20	Uniprot
P26038 (MSN)	T558	Uniprot
P29474 (NOS3)	T495	Uniprot
P35241 (RDX)	T564	Uniprot
P35568 (IRS1)	S636	Uniprot
P35568 (IRS1)	S974	Uniprot
P35611 (ADD1)	T445	Uniprot
P35611 (ADD1)	T480	Uniprot
P51911 (CNN1)	T170	Uniprot
P51911 (CNN1)	S175	Uniprot
P51911 (CNN1)	T180	Uniprot
P51911 (CNN1)	T184	Uniprot
P51911 (CNN1)	T259	Uniprot
P53671 (LIMK2)	T505	Uniprot
P56817 (BACE1)	S498	Uniprot
P68104 (EEF1A1)	T432	Uniprot
Q15306 (IRF4)	S447	Uniprot
Q15306 (IRF4)	S448	Uniprot
Q15417 (CNN3)	S293	Uniprot
Q16555 (DPYSL2)	T555	Uniprot
Q92673 (SORL1)	S2206	Uniprot
Q96A00 (PPP1R14A)	T38	Uniprot
Q96C90 (PPP1R14B)	T57	Uniprot
Q99962 (SH3GL2)	T14	Uniprot

Research Backgrounds

O75116_ROCK2_HUMAN

Function:

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.

PTMs:

Phosphorylation at Tyr-722 reduces its binding to RHOA and is crucial for focal adhesion dynamics. Dephosphorylation by PTPN11 stimulates its RHOA binding activity.

Cleaved by granzyme B during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.

Subcellular Location:

Cytoplasm. Cell membrane>Peripheral membrane protein. Nucleus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Cytoplasmic, and associated with actin microfilaments and the plasma membrane.

Tissue Specificity:

Expressed in the brain (at protein level).

Subunit Structure:

Homodimer (By similarity). Interacts with IRS1 (By similarity). Interacts with RAF1 (By similarity). Interacts with RHOA (activated by GTP), RHOB and RHOC (By similarity). Interacts with PPP1R12A. Interacts with EP300. Interacts with CHORDC1. Interacts with BRCA2. Interacts with NPM1; this interaction enhances ROCK2 activity. Interacts with SORL1.

Family&Domains:

An interaction between Thr-414 and Asp-48 is essential for kinase activity and dimerization.

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)

· Organismal Systems > Development > Axon guidance. (View pathway)

· Organismal Systems > Immune system > Platelet activation. (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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AFfirm™ ROCK2 Mouse Monoclonal Antibody - #BF8213