AFfirm™ Connexin 43 / GJA1 Mouse Monoclonal Antibody - #BF8212
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Fold/Unfold
Connexin 43; Connexin-43; Cx 43; Cx43; CXA1_HUMAN; DFNB38; Gap junction 43 kDa heart protein; Gap junction alpha-1 protein; Gap junction protein alpha 1 43kDa (connexin 43); Gap junction protein alpha 1 43kDa; Gap junction protein alpha like; GJA 1; Gja1; GJAL; ODD; ODDD; ODOD; SDTY3;
Immunogens
- P17302 CXA1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGDWSALGKLLDKVQAYSTAGGKVWLSVLFIFRILLLGTAVESAWGDEQSAFRCNTQQPGCENVCYDKSFPISHVRFWVLQIIFVSVPTLLYLAHVFYVMRKEEKLNKKEEELKVAQTDGVNVDMHLKQIEIKKFKYGIEEHGKVKMRGGLLRTYIISILFKSIFEVAFLLIQWYIYGFSLSAVYTCKRDPCPHQVDCFLSRPTEKTIFIIFMLVVSLVSLALNIIELFYVFFKGVKDRVKGKSDPYHATSGALSPAKDCGSQKYAYFNGCSSPTAPLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDDNQNSKKLAAGHELQPLAIVDQRPSSRASSRASSRPRPDDLEI
PTMs - P17302 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K9 | Ubiquitination | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| K128 | Ubiquitination | Uniprot | |
| K136 | Ubiquitination | Uniprot | |
| Y137 | Phosphorylation | Uniprot | |
| K144 | Ubiquitination | Uniprot | |
| R148 | Methylation | Uniprot | |
| K243 | Ubiquitination | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| Y247 | Phosphorylation | P12931 (SRC) | Uniprot |
| T250 | Phosphorylation | Uniprot | |
| S251 | Phosphorylation | Uniprot | |
| S255 | Phosphorylation | P28482 (MAPK1) , P06493 (CDK1) , P27361 (MAPK3) , Q13164 (MAPK7) | Uniprot |
| K258 | Ubiquitination | Uniprot | |
| S262 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| K264 | Ubiquitination | Uniprot | |
| Y265 | Phosphorylation | P12931 (SRC) | Uniprot |
| Y267 | Phosphorylation | Uniprot | |
| S272 | Phosphorylation | Uniprot | |
| T275 | Phosphorylation | Uniprot | |
| S279 | Phosphorylation | Q16539 (MAPK14) , P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| S282 | Phosphorylation | P28482 (MAPK1) , Q16539 (MAPK14) , P27361 (MAPK3) , Q13164 (MAPK7) | Uniprot |
| Y286 | Phosphorylation | Uniprot | |
| K287 | Ubiquitination | Uniprot | |
| T290 | Phosphorylation | Uniprot | |
| S296 | Phosphorylation | Uniprot | |
| S297 | Phosphorylation | Uniprot | |
| Y301 | Phosphorylation | Uniprot | |
| K303 | Ubiquitination | Uniprot | |
| S306 | Phosphorylation | Uniprot | |
| Y313 | Phosphorylation | Uniprot | |
| S314 | Phosphorylation | Uniprot | |
| S325 | Phosphorylation | P48730 (CSNK1D) | Uniprot |
| T326 | Phosphorylation | Uniprot | |
| S328 | Phosphorylation | P48730 (CSNK1D) | Uniprot |
| S330 | Phosphorylation | P48730 (CSNK1D) | Uniprot |
| S344 | Phosphorylation | Uniprot | |
| K345 | Ubiquitination | Uniprot | |
| K346 | Ubiquitination | Uniprot | |
| S364 | Phosphorylation | Uniprot | |
| S365 | Phosphorylation | Q02156 (PRKCE) , P17612 (PRKACA) | Uniprot |
| S368 | Phosphorylation | P17252 (PRKCA) , P17612 (PRKACA) , Q02156 (PRKCE) , P05771 (PRKCB) , P05129 (PRKCG) , Q05655 (PRKCD) | Uniprot |
| S369 | Phosphorylation | P31749 (AKT1) , Q02156 (PRKCE) , P17612 (PRKACA) | Uniprot |
| S372 | Phosphorylation | Uniprot | |
| S373 | Phosphorylation | P17612 (PRKACA) , P31749 (AKT1) , Q02156 (PRKCE) | Uniprot |
Research Backgrounds
Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). May play a role in cell growth inhibition through the regulation of NOV expression and localization. Plays an essential role in gap junction communication in the ventricles (By similarity).
Phosphorylated at Ser-368 by PRKCG; phosphorylation induces disassembly of gap junction plaques and inhibition of gap junction activity (By similarity). Phosphorylation at Ser-325, Ser-328 and Ser-330 by CK1 modulates gap junction assembly. Phosphorylation at Ser-368 by PRKCD triggers its internalization into small vesicles leading to proteasome-mediated degradation (By similarity).
Sumoylated with SUMO1, SUMO2 and SUMO3, which may regulate the level of functional Cx43 gap junctions at the plasma membrane. May be desumoylated by SENP1 or SENP2.
S-nitrosylation at Cys-271 is enriched at the muscle endothelial gap junction in arteries, it augments channel permeability and may regulate of smooth muscle cell to endothelial cell communication.
Acetylated in the developing cortex; leading to delocalization from the cell membrane.
Cell membrane>Multi-pass membrane protein. Cell junction>Gap junction. Endoplasmic reticulum.
Note: Localizes at the intercalated disk (ICD) in cardiomyocytes and the proper localization at ICD is dependent on TMEM65.
Expressed in the heart and fetal cochlea.
A connexon is composed of a hexamer of connexins. Interacts (via C-terminus) with TJP1 (By similarity). Interacts (via C-terminus) with SRC (via SH3 domain) (By similarity). Interacts (not ubiquitinated) with UBQLN4 (via UBA domain) (By similarity). Interacts with SGSM3 and CNST (By similarity). Interacts with RIC1/CIP150. Interacts with CSNK1D. Interacts with NOV. Interacts with TMEM65 (By similarity).
Belongs to the connexin family. Alpha-type (group II) subfamily.
Research Fields
· Cellular Processes > Cellular community - eukaryotes > Gap junction. (View pathway)
· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).
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