AFfirm™ CD34 Mouse Monoclonal Antibody - #BF8200

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Product: CD34 Mouse Monoclonal Antibody
Catalog: BF8200
Description: Mouse monoclonal antibody to CD34
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Rabbit, Dog
Mol.Wt.: 120 kDa; 41kD(Calculated).
Uniprot: P28906

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Monoclonal [AFfirm8200]
Specificity:
CD34 Antibody detects endogenous levels of total CD34.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CD34; CD34 antigen; CD34 molecule; CD34_HUMAN; Cluster designation 34; Hematopoietic progenitor cell antigen CD34; HPCA1; Mucosialin; OTTHUMP00000034733; OTTHUMP00000034734;

Immunogens

Immunogen:
Uniprot:

P28906(CD34_HUMAN) >>Visit HPA database.

Gene(ID):
CD34(947)
Expression:
P28906 CD34_HUMAN:

Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.

Description:
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
Sequence:
MLVRRGARAGPRMPRGWTALCLLSLLPSGFMSLDNNGTATPELPTQGTFSNVSTNVSYQETTTPSTLGSTSLHPVSQHGNEATTNITETTVKFTSTSVITSVYGNTNSSVQSQTSVISTVFTTPANVSTPETTLKPSLSPGNVSDLSTTSTSLATSPTKPYTSSSPILSDIKAEIKCSGIREVKLTQGICLEQNKTSSCAEFKKDRGEGLARVLCGEEQADADAGAQVCSLLLAQSEVRPQCLLLVLANRTEISSKLQLMKKHQSDLKKLGILDFTEQDVASHQSYSQKTLIALVTSGALLAVLGITGYFLMNRRSWSPTGERLGEDPYYTENGGGQGYSSGPGTSPEAQGKASVNRGAQENGTGQATSRNGHSARQHVVADTEL

PTMs - P28906 As Substrate

Site PTM Type Enzyme
S198 Phosphorylation
K268 Acetylation
S282 Phosphorylation
Y286 Phosphorylation
Y329 Phosphorylation
Y330 Phosphorylation
T331 Phosphorylation
Y339 Phosphorylation
S341 Phosphorylation
S346 Phosphorylation P31751 (AKT2)
S354 Phosphorylation

Research Backgrounds

Function:

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

PTMs:

Highly glycosylated.

Phosphorylated on serine residues by PKC.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.

Family&Domains:

Belongs to the CD34 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

References

1). Ethyl cinnamate suppresses tumor growth through anti-angiogenesis by attenuating VEGFR2 signal pathway in colorectal cancer. Journal of ethnopharmacology, 2024 (PubMed: 38360380) [IF=5.4]

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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