Vimentin Mouse Monoclonal Antibody | Affinity Biosciences

Product:	Vimentin Mouse Monoclonal Antibody
Catalog:	BF8175
Description:	Mouse monoclonal antibody to Vimentin
Application:	WB
Reactivity:	Human, Rat
Prediction:	Pig, Bovine, Sheep, Dog
Mol.Wt.:	54kDa; 54kD(Calculated).
Uniprot:	P08670

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Rat

Clonality:

Monoclonal [AFfirm8175]

Specificity:

Vimentin Antibody detects endogenous levels of total Vimentin.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CTRCT30; Epididymis luminal protein 113; FLJ36605; HEL113; VIM; VIME_HUMAN; Vimentin;

Immunogens

Immunogen:

Uniprot:

P08670(VIME_HUMAN) >>Visit HPA database.

Gene(ID):

VIM(7431)

Expression:

P08670 VIME_HUMAN:

Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

Description:

VIM is an intermediate filament protein. Intermediate filament proteins are expressed in a tissue-specific manner. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.

Sequence:

P08670 VIME_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSTRSVSSSSYRRMFGGPGTASRPSSSRSYVTTSTRTYSLGSALRPSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAENTLQSFRQDVDNASLARLDLERKVESLQEEIAFLKKLHEEEIQELQAQIQEQHVQIDVDVSKPDLTAALRDVRQQYESVAAKNLQEAEEWYKSKFADLSEAANRNNDALRQAKQESTEYRRQVQSLTCEVDALKGTNESLERQMREMEENFAVEAANYQDTIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRISLPLPNFSSLNLRETNLDSLPLVDTHSKRTLLIKTVETRDGQVINETSQHHDDLE

PTMs - P08670 As Substrate

P08670

Site	PTM Type	Enzyme	Source
S2	Phosphorylation		Uniprot
T3	Phosphorylation		Uniprot
R4	Methylation		Uniprot
S5	Phosphorylation	P17252 (PRKCA)	Uniprot
S7	O-Glycosylation		Uniprot
S7	Phosphorylation	P17252 (PRKCA) , Q96GD4 (AURKB) , Q02156 (PRKCE) , P17612 (PRKACA)	Uniprot
S8	Phosphorylation	P17252 (PRKCA)	Uniprot
S9	Phosphorylation	P17252 (PRKCA)	Uniprot
S10	Phosphorylation	P17252 (PRKCA)	Uniprot
Y11	Phosphorylation		Uniprot
R12	Methylation		Uniprot
R13	Methylation		Uniprot
T20	Phosphorylation		Uniprot
S22	Phosphorylation		Uniprot
R23	Methylation		Uniprot
S25	Phosphorylation	P17252 (PRKCA) , Q96GD4 (AURKB) , P17612 (PRKACA)	Uniprot
S26	Phosphorylation	Q13153 (PAK1) , P17252 (PRKCA) , Q13177 (PAK2)	Uniprot
S27	Phosphorylation		Uniprot
R28	Methylation		Uniprot
S29	Phosphorylation		Uniprot
Y30	Phosphorylation		Uniprot
T32	Phosphorylation		Uniprot
T33	O-Glycosylation		Uniprot
T33	Phosphorylation		Uniprot
S34	O-Glycosylation		Uniprot
S34	Phosphorylation	P17252 (PRKCA)	Uniprot
T35	Phosphorylation		Uniprot
R36	Methylation		Uniprot
T37	Phosphorylation		Uniprot
Y38	Phosphorylation		Uniprot
S39	Phosphorylation	P49137 (MAPKAPK2) , Q13177 (PAK2) , P17612 (PRKACA) , O75116 (ROCK2) , P31749 (AKT1) , Q13153 (PAK1) , Q96GD4 (AURKB) , P17252 (PRKCA)	Uniprot
S42	Phosphorylation	P17252 (PRKCA)	Uniprot
R45	Methylation		Uniprot
S47	Phosphorylation	P17612 (PRKACA) , Q96GD4 (AURKB)	Uniprot
T48	Phosphorylation		Uniprot
S49	Phosphorylation		Uniprot
R50	Methylation		Uniprot
S51	Phosphorylation	Q13177 (PAK2) , Q13153 (PAK1) , P49137 (MAPKAPK2)	Uniprot
Y53	Phosphorylation		Uniprot
S55	O-Glycosylation		Uniprot
S55	Phosphorylation	P24941 (CDK2) , P06493 (CDK1)	Uniprot
S56	Phosphorylation	P78527 (PRKDC) , P06493 (CDK1) , P49137 (MAPKAPK2) , Q13153 (PAK1) , P24941 (CDK2) , Q00535 (CDK5)	Uniprot
Y61	Phosphorylation		Uniprot
T63	Phosphorylation		Uniprot
R64	Methylation		Uniprot
S65	Phosphorylation	Q96GD4 (AURKB)	Uniprot
S66	Phosphorylation	Q96GD4 (AURKB) , Q13177 (PAK2) , Q13153 (PAK1)	Uniprot
R69	Methylation		Uniprot
R71	Methylation		Uniprot
S72	Phosphorylation	P17612 (PRKACA) , Q96GD4 (AURKB) , Q13464 (ROCK1) , O75116 (ROCK2)	Uniprot
S73	Phosphorylation	Q13177 (PAK2) , Q96GD4 (AURKB) , P17612 (PRKACA) , Q13153 (PAK1)	Uniprot
S83	Phosphorylation	Q13557 (CAMK2D) , Q9UQM7 (CAMK2A) , P49137 (MAPKAPK2) , P53350 (PLK1)	Uniprot
S87	Phosphorylation	Q96GD4 (AURKB)	Uniprot
K97	Ubiquitination		Uniprot
T99	Phosphorylation		Uniprot
T101	Phosphorylation		Uniprot
K104	Acetylation		Uniprot
K104	Ubiquitination		Uniprot
R113	Methylation		Uniprot
Y117	Phosphorylation		Uniprot
K120	Acetylation		Uniprot
K120	Methylation		Uniprot
K120	Ubiquitination		Uniprot
K129	Acetylation		Uniprot
K129	Ubiquitination		Uniprot
K139	Acetylation		Uniprot
K139	Ubiquitination		Uniprot
K143	Ubiquitination		Uniprot
S144	Phosphorylation		Uniprot
Y150	Phosphorylation		Uniprot
R158	Methylation		Uniprot
K168	Acetylation		Uniprot
K168	Ubiquitination		Uniprot
R184	Methylation		Uniprot
K188	Ubiquitination		Uniprot
T202	Phosphorylation		Uniprot
S205	Phosphorylation		Uniprot
S214	Phosphorylation		Uniprot
K223	Ubiquitination		Uniprot
S226	Phosphorylation		Uniprot
K235	Acetylation		Uniprot
K235	Ubiquitination		Uniprot
K236	Acetylation		Uniprot
K236	Ubiquitination		Uniprot
S261	Phosphorylation		Uniprot
K262	Ubiquitination		Uniprot
T266	Phosphorylation		Uniprot
Y276	Phosphorylation		Uniprot
S278	Phosphorylation		Uniprot
K282	Acetylation		Uniprot
K282	Ubiquitination		Uniprot
Y291	Phosphorylation		Uniprot
K292	Acetylation		Uniprot
K292	Ubiquitination		Uniprot
K294	Acetylation		Uniprot
K294	Ubiquitination		Uniprot
S299	Phosphorylation		Uniprot
R310	Methylation		Uniprot
K313	Acetylation		Uniprot
K313	Sumoylation		Uniprot
K313	Ubiquitination		Uniprot
S316	Phosphorylation		Uniprot
T317	Phosphorylation		Uniprot
S325	Phosphorylation		Uniprot
T327	Phosphorylation		Uniprot
C328	S-Nitrosylation		Uniprot
K334	Acetylation		Uniprot
K334	Ubiquitination		Uniprot
T336	Phosphorylation		Uniprot
S339	Phosphorylation		Uniprot
Y358	Phosphorylation		Uniprot
T361	Phosphorylation		Uniprot
K373	Acetylation		Uniprot
K373	Ubiquitination		Uniprot
R381	Methylation		Uniprot
Y383	Phosphorylation		Uniprot
Y400	Phosphorylation		Uniprot
R401	Methylation		Uniprot
K402	Acetylation		Uniprot
K402	Sumoylation		Uniprot
K402	Ubiquitination		Uniprot
S409	Phosphorylation		Uniprot
R410	Methylation		Uniprot
S412	Phosphorylation		Uniprot
S419	Phosphorylation		Uniprot
S420	Phosphorylation		Uniprot
T426	Phosphorylation		Uniprot
S430	Phosphorylation	P78527 (PRKDC)	Uniprot
T436	Phosphorylation		Uniprot
S438	Phosphorylation		Uniprot
K439	Acetylation		Uniprot
K439	Ubiquitination		Uniprot
T441	Phosphorylation		Uniprot
K445	Acetylation		Uniprot
K445	Methylation		Uniprot
K445	Sumoylation		Uniprot
K445	Ubiquitination		Uniprot
T446	Phosphorylation		Uniprot
T449	Phosphorylation		Uniprot
T458	Phosphorylation	P00540 (MOS)	Uniprot
S459	Phosphorylation	P78527 (PRKDC) , P53350 (PLK1) , P00540 (MOS)	Uniprot

Research Backgrounds

P08670_VIME_HUMAN

Function:

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

PTMs:

Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33. Phosphorylated on tyrosine residues by SRMS.

O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.

S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

Subcellular Location:

Cytoplasm. Cytoplasm>Cytoskeleton. Nucleus matrix.

Tissue Specificity:

Subunit Structure:

Homopolymer assembled from elementary dimers. Interacts with LGSN and SYNM. Interacts (via rod region) with PLEC (via CH 1 domain) (By similarity). Interacts with SLC6A4. Interacts with STK33. Interacts with LARP6. Interacts with RAB8B (By similarity). Interacts with TOR1A; the interaction associates TOR1A with the cytoskeleton. Interacts with TOR1AIP1. Interacts with BCAS3. Interacts with DIAPH1. Identified in complexes that contain VIM, EZR, AHNAK, BFSP1, BFSP2, ANK2, PLEC, PRX and spectrin (By similarity). Interacts with EPPK1; interaction is dependent of higher-order structure of intermediate filament. Interacts with the non-receptor tyrosine kinase SRMS; the interaction leads to phosphorylation of VIM. Interacts with NOD2. Interacts (via head region) with CORO1C (By similarity). Interacts with HDGF (isoform 2).

(Microbial infection) Interacts with HCV core protein.

Family&Domains:

The central alpha-helical coiled-coil IF rod domain mediates elementary homodimerization.

The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.

Belongs to the intermediate filament family.

Research Fields

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

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AFfirm™ Vimentin Mouse Monoclonal Antibody - #BF8175