PDCD4 Mouse Monoclonal Antibody | Affinity Biosciences

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Western blot analysis of extracts from MCF7 cells, using PDCD4 Mouse Monoclonal Antibody.

Western blot analysis of extracts from Mouse heart, using PDCD4 Mouse Monoclonal Antibody.

BF8141 at 1/100 staining mouse testis tissue by IHC-P.

Product:	PDCD4 Mouse Monoclonal Antibody
Catalog:	BF8141
Description:	Mouse monoclonal antibody to PDCD4
Application:	WB IHC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	51kDa; 52kD(Calculated).
Uniprot:	Q53EL6

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Monoclonal [AFfirm8141]

Specificity:

PDCD4 Antibody detects endogenous levels of total PDCD4.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Death up-regulated gene protein; Dug; H731; Ma3; MGC33046; MGC33047; Neoplastic transformation inhibitor; Neoplastic transformation inhibitor protein; Nuclear antigen H731; Nuclear antigen H731 like; Nuclear antigen H731 like protein; Nuclear antigen H731-like; PDCD 4; Pdcd4; PDCD4_HUMAN; Programmed cell death 4; programmed cell death 4 (neoplastic transformation inhibitor); Programmed cell death protein 4; Protein 197/15a; Protein MA-3; Tis; Topoisomerase-inhibitor suppressed protein;

Immunogens

Immunogen:

Uniprot:

Q53EL6(PDCD4_HUMAN) >>Visit HPA database.

Gene(ID):

PDCD4(27250)

Expression:

Q53EL6 PDCD4_HUMAN:

Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.

Description:

This gene encodes a protein localized to the nucleus in proliferating cells. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two transcripts encoding different isoforms have been identified.

Sequence:

Q53EL6 PDCD4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDVENEQILNVNPADPDNLSDSLFSGDEENAGTEEIKNEINGNWISASSINEARINAKAKRRLRKNSSRDSGRGDSVSDSGSDALRSGLTVPTSPKGRLLDRRSRSGKGRGLPKKGGAGGKGVWGTPGQVYDVEEVDVKDPNYDDDQENCVYETVVLPLDERAFEKTLTPIIQEYFEHGDTNEVAEMLRDLNLGEMKSGVPVLAVSLALEGKASHREMTSKLLSDLCGTVMSTTDVEKSFDKLLKDLPELALDTPRAPQLVGQFIARAVGDGILCNTYIDSYKGTVDCVQARAALDKATVLLSMSKGGKRKDSVWGSGGGQQSVNHLVKEIDMLLKEYLLSGDISEAEHCLKELEVPHFHHELVYEAIIMVLESTGESTFKMILDLLKSLWKSSTITVDQMKRGYERIYNEIPDINLDVPHSYSVLERFVEECFQAGIISKQLRDLCPSRGRKRFVSEGDGGRLKPESY

PTMs - Q53EL6 As Substrate

Q53EL6

Site	PTM Type	Enzyme	Source
M1	Acetylation		Uniprot
S20	Phosphorylation		Uniprot
S22	Phosphorylation		Uniprot
S25	Phosphorylation		Uniprot
S46	Phosphorylation		Uniprot
K58	Ubiquitination		Uniprot
K60	Ubiquitination		Uniprot
S67	Phosphorylation	P31749 (AKT1) , P23443 (RPS6KB1) , Q9UBS0 (RPS6KB2)	Uniprot
S68	Phosphorylation		Uniprot
S71	Phosphorylation		Uniprot
R73	Methylation		Uniprot
S76	Phosphorylation		Uniprot
S78	Phosphorylation		Uniprot
S80	Phosphorylation		Uniprot
S82	Phosphorylation		Uniprot
S87	Phosphorylation		Uniprot
T90	Phosphorylation		Uniprot
T93	Phosphorylation		Uniprot
S94	Phosphorylation		Uniprot
K96	Ubiquitination		Uniprot
K121	Ubiquitination		Uniprot
T126	Phosphorylation		Uniprot
Y131	Phosphorylation		Uniprot
Y143	Phosphorylation		Uniprot
Y152	Phosphorylation		Uniprot
K166	Ubiquitination		Uniprot
K212	Ubiquitination		Uniprot
K238	Ubiquitination		Uniprot
K242	Ubiquitination		Uniprot
K245	Ubiquitination		Uniprot
T254	Phosphorylation		Uniprot
R267	Methylation		Uniprot
K283	Ubiquitination		Uniprot
K297	Ubiquitination		Uniprot
T299	Phosphorylation		Uniprot
S303	Phosphorylation		Uniprot
K306	Ubiquitination		Uniprot
K311	Ubiquitination		Uniprot
S313	Phosphorylation		Uniprot
S317	Phosphorylation		Uniprot
S323	Phosphorylation		Uniprot
S345	Phosphorylation		Uniprot
K392	Ubiquitination		Uniprot
K402	Ubiquitination		Uniprot
Y405	Phosphorylation		Uniprot
S440	Phosphorylation		Uniprot
S457	Phosphorylation	P31749 (AKT1)	Uniprot
K465	Sumoylation		Uniprot
S468	Phosphorylation		Uniprot

Research Backgrounds

Q53EL6_PDCD4_HUMAN

Function:

Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA (By similarity).

PTMs:

Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.

Phosphorylated at Ser-67 by RPS6KB1 in response to mitogens; phosphorylation promotes proteasomal degradation of PDCD4.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Shuttles between the nucleus and cytoplasm (By similarity). Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457 (PubMed:16357133).

Tissue Specificity:

Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.

Subunit Structure:

Interacts (via MI domains) with EIF4A2 (By similarity). Interacts (via MI domains) with EIF4A1 (via N-terminal domain). Heterotrimer with EIF4A1; one molecule of PDCD4 binds two molecules of EIF4A1. Interacts with EIF4G1. May form a complex with EIF4A1 and EIF4G1. The interaction between PDCD4 and EIF4A1 interferes with the interaction between EIF4A1 and EIF4G. When phosphorylated, interacts with BTRC and FBXW11.

Family&Domains:

Binds EIF4A1 via both MI domains.

Belongs to the PDCD4 family.

Research Fields

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

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For Research Use Only.
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AFfirm™ PDCD4 Mouse Monoclonal Antibody - #BF8141