AFfirm™ ATF1 Mouse Monoclonal Antibody - #BF8140

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Product: ATF1 Mouse Monoclonal Antibody
Catalog: BF8140
Description: Mouse monoclonal antibody to ATF1
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 29kDa; 29kD(Calculated).
Uniprot: P18846

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   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Monoclonal [AFfirm8140]
Specificity:
ATF1 Antibody detects endogenous levels of total ATF1.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Activating transcription factor 1; ATF 1; atf1; ATF1 EWS fusion gene; ATF1 FUS fusion gene; ATF1_HUMAN; cAMP dependent transcription factor 1; cAMP-dependent transcription factor ATF-1; Cyclic AMP dependent transcription factor ATF 1; Cyclic AMP dependent transcription factor ATF1; Cyclic AMP-dependent transcription factor ATF-1; EWS AFT1; FUS ATF 1; FUS/ATF 1; Protein TREB36; RNA binding protein activating transcription factor 1 fusion protein; TREB 36; TREB36; TREB36 protein;

Immunogens

Immunogen:

A synthesized peptide derived from human ATF1, corresponding to a region within the internal amino acids.

Uniprot:

P18846(ATF1_HUMAN) >>Visit HPA database.

Gene(ID):
ATF1(466)
Description:
ATF-1 a transcription factor that is a member of the leucine zipper family. Forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. Binds the Tax-responsive element (TRE) of HTLV-I. Activated downstream of IL-1. c-Src and TRAF6 are mediators of IL-1-induced AP-1 activation.
Sequence:
MEDSHKSTTSETAPQPGSAVQGAHISHIAQQVSSLSESEESQDSSDSIGSSQKAHGILARRPSYRKILKDLSSEDTRGRKGDGENSGVSAAVTSMSVPTPIYQTSSGQYIAIAPNGALQLASPGTDGVQGLQTLTMTNSGSTQQGTTILQYAQTSDGQQILVPSNQVVVQTASGDMQTYQIRTTPSATSLPQTVVMTSPVTLTSQTTKTDDPQLKREIRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKTLKDLYSNKSV

Research Backgrounds

Function:

This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation.

PTMs:

Phosphorylated at Ser-198 by HIPK2 in response to genotoxic stress. This phosphorylation promotes transcription repression of FTH1 and other antioxidant detoxification genes. The CDK3-mediated phosphorylation at Ser-63 promotes its transactivation and transcriptional activities. Phosphorylated at Ser-63 by RPS6KA4 and RPS6KA5 in response to mitogenic or stress stimuli.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the bZIP family. ATF subfamily.

Research Fields

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

Restrictive clause

 

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