RanBP1 Mouse Monoclonal Antibody | Affinity Biosciences

Product:	RanBP1 Mouse Monoclonal Antibody
Catalog:	BF8086
Description:	Mouse monoclonal antibody to RanBP1
Application:	WB
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	23 kDa; 23kD(Calculated).
Uniprot:	P43487

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:1000-10000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Monoclonal [AFfirm8086]

Specificity:

RanBP1 Antibody detects endogenous levels of total RanBP1.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

HTF9A; RAN binding protein 1; Ran specific GTPase activating protein; Ran-binding protein 1; Ran-specific GTPase-activating protein; RANBP1; RANG_HUMAN;

Immunogens

Immunogen:

Uniprot:

P43487(RANG_HUMAN) >>Visit HPA database.

Gene(ID):

RANBP1(5902)

Sequence:

P43487 RANG_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAAKDTHEDHDTSTENTDESNHDPQFEPIVSLPEQEIKTLEEDEEELFKMRAKLFRFASENDLPEWKERGTGDVKLLKHKEKGAIRLLMRRDKTLKICANHYITPMMELKPNAGSDRAWVWNTHADFADECPKPELLAIRFLNAENAQKFKTKFEECRKEIEEREKKAGSGKNDHAEKVAEKLEALSVKEETKEDAEEKQ

PTMs - P43487 As Substrate

P43487

Site	PTM Type	Source
A2	Acetylation	Uniprot
K5	Acetylation	Uniprot
T7	Phosphorylation	Uniprot
T13	Phosphorylation	Uniprot
S14	Phosphorylation	Uniprot
T15	Phosphorylation	Uniprot
T18	Phosphorylation	Uniprot
S21	Phosphorylation	Uniprot
S32	Phosphorylation	Uniprot
K39	Ubiquitination	Uniprot
K50	Ubiquitination	Uniprot
K54	Acetylation	Uniprot
S60	Phosphorylation	Uniprot
K68	Acetylation	Uniprot
K68	Methylation	Uniprot
K68	Ubiquitination	Uniprot
K76	Ubiquitination	Uniprot
K79	Acetylation	Uniprot
Y103	Phosphorylation	Uniprot
T105	Phosphorylation	Uniprot
K111	Acetylation	Uniprot
K111	Ubiquitination	Uniprot
K134	Acetylation	Uniprot
K134	Ubiquitination	Uniprot
K150	Acetylation	Uniprot
K150	Ubiquitination	Uniprot
K152	Acetylation	Uniprot
K154	Acetylation	Uniprot
K154	Ubiquitination	Uniprot
K160	Acetylation	Uniprot
K183	Acetylation	Uniprot
K183	Ubiquitination	Uniprot
S188	Phosphorylation	Uniprot
K190	Acetylation	Uniprot
K190	Sumoylation	Uniprot
K190	Ubiquitination	Uniprot
T193	Phosphorylation	Uniprot
K194	Acetylation	Uniprot
K200	Acetylation	Uniprot

Research Backgrounds

P43487_RANG_HUMAN

Function:

Plays a role in RAN-dependent nucleocytoplasmic transport. Alleviates the TNPO1-dependent inhibition of RAN GTPase activity and mediates the dissociation of RAN from proteins involved in transport into the nucleus (By similarity). Induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins. Promotes the disassembly of the complex formed by RAN and importin beta. Promotes dissociation of RAN from a complex with KPNA2 and CSE1L (By similarity). Required for normal mitotic spindle assembly and normal progress through mitosis via its effect on RAN. Does not increase the RAN GTPase activity by itself, but increases GTP hydrolysis mediated by RANGAP1. Inhibits RCC1-dependent exchange of RAN-bound GDP by GTP.

Subunit Structure:

Interacts with RAN (via C-terminus of GTP-bound form) but not with GDP-bound RAN. Identified in a complex composed of RAN, RANGAP1 and RANBP1. Identified in a complex that contains TNPO1, RAN and RANBP1. Identified in a complex that contains CSE1L, KPNA2, RAN and RANBP1 (By similarity). Identified in a complex with nucleotide-free RAN and RCC1.

Family&Domains:

Belongs to the RANBP1 family.

Research Fields

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

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For Research Use Only.
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AFfirm™ RanBP1 Mouse Monoclonal Antibody - #BF8086