Zyxin Mouse Monoclonal Antibody | Affinity Biosciences

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Western blot analysis of extracts from various samples, using Zyxin Mouse Monoclonal Antibody.

Product:	Zyxin Mouse Monoclonal Antibody
Catalog:	BF8083
Description:	Mouse monoclonal antibody to Zyxin
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	80kDa,70kDa; 61kD(Calculated).
Uniprot:	Q15942

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Monoclonal [AFfirm8083]

Specificity:

Zyxin Antibody detects endogenous levels of total Zyxin.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ESP 2; ESP2; HED 2; HED2; ZYX; ZYX protein; Zyxin 2; Zyxin2;

Immunogens

Immunogen:

A synthesized peptide derived from human Zyxin, corresponding to a region within the internal amino acids.

Uniprot:

Q15942(ZYX_HUMAN) >>Visit HPA database.

Gene(ID):

ZYX(7791)

Description:

The zyxin family of proteins includes LIMD1, ajuba, trip6 and zyxin, each of which contains three LIM domains at the carboxy-terminus. Zyxin family members associate with the actin cytoskeleton and are components of both the cell-cell junction adhesive complex and the integrin-mediated adhesive complex. They shuttle in and out of the nucleus where they may function in transcriptional activation (1).Zyxin is involved in the regulation of mechanical force-induced actin polymerization at focal adhesions (2), and in regulation of adhesion and migration, possibly through recruitment of Ena/VASP proteins to focal adhesions (3). Zyxin interacts with and may regulate the function of the tumor suppressor myopodin, which inhibits tumor growth and metastasis (4).

Sequence:

Q15942 ZYX_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAPRPSPAISVSVSAPAFYAPQKKFGPVVAPKPKVNPFRPGDSEPPPAPGAQRAQMGRVGEIPPPPPEDFPLPPPPLAGDGDDAEGALGGAFPPPPPPIEESFPPAPLEEEIFPSPPPPPEEEGGPEAPIPPPPQPREKVSSIDLEIDSLSSLLDDMTKNDPFKARVSSGYVPPPVATPFSSKSSTKPAAGGTAPLPPWKSPSSSQPLPQVPAPAQSQTQFHVQPQPQPKPQVQLHVQSQTQPVSLANTQPRGPPASSPAPAPKFSPVTPKFTPVASKFSPGAPGGSGSQPNQKLGHPEALSAGTGSPQPPSFTYAQQREKPRVQEKQHPVPPPAQNQNQVRSPGAPGPLTLKEVEELEQLTQQLMQDMEHPQRQNVAVNELCGRCHQPLARAQPAVRALGQLFHIACFTCHQCAQQLQGQQFYSLEGAPYCEGCYTDTLEKCNTCGEPITDRMLRATGKAYHPHCFTCVVCARPLEGTSFIVDQANRPHCVPDYHKQYAPRCSVCSEPIMPEPGRDETVRVVALDKNFHMKCYKCEDCGKPLSIEADDNGCFPLDGHVLCRKCHTARAQT

PTMs - Q15942 As Substrate

Q15942

Site	PTM Type	Enzyme	Source
A2	Acetylation		Uniprot
R5	Methylation		Uniprot
S7	Phosphorylation		Uniprot
S13	Phosphorylation		Uniprot
S15	Phosphorylation		Uniprot
Y20	Phosphorylation		Uniprot
K24	Acetylation		Uniprot
K25	Acetylation		Uniprot
K25	Methylation		Uniprot
S44	Phosphorylation		Uniprot
S116	Phosphorylation		Uniprot
S142	Phosphorylation	P31749 (AKT1)	Uniprot
S143	Phosphorylation		Uniprot
S150	Phosphorylation		Uniprot
K165	Acetylation		Uniprot
K165	Methylation		Uniprot
K165	Ubiquitination		Uniprot
S169	Phosphorylation		Uniprot
S170	Phosphorylation		Uniprot
Y172	Phosphorylation		Uniprot
T179	Phosphorylation		Uniprot
K201	Methylation		Uniprot
S202	Phosphorylation		Uniprot
S206	Phosphorylation		Uniprot
T250	Phosphorylation		Uniprot
S258	Phosphorylation		Uniprot
S259	Phosphorylation		Uniprot
K265	Acetylation		Uniprot
K265	Ubiquitination		Uniprot
S267	Phosphorylation		Uniprot
T270	Phosphorylation		Uniprot
K272	Acetylation		Uniprot
K272	Methylation		Uniprot
K272	Ubiquitination		Uniprot
T274	Phosphorylation		Uniprot
S278	Phosphorylation		Uniprot
K279	Acetylation		Uniprot
K279	Methylation		Uniprot
K279	Ubiquitination		Uniprot
S281	Phosphorylation		Uniprot
S288	Phosphorylation		Uniprot
S290	Phosphorylation		Uniprot
K295	Ubiquitination		Uniprot
S303	Phosphorylation	P24941 (CDK2)	Uniprot
T306	Phosphorylation	P24941 (CDK2)	Uniprot
S308	Phosphorylation	P24941 (CDK2)	Uniprot
S313	Phosphorylation		Uniprot
T315	Phosphorylation		Uniprot
Y316	Phosphorylation		Uniprot
K328	Ubiquitination		Uniprot
R343	Methylation		Uniprot
S344	Phosphorylation		Uniprot
T352	Phosphorylation		Uniprot
S505	Phosphorylation		Uniprot
K528	Acetylation		Uniprot

Research Backgrounds

Q15942_ZYX_HUMAN

Function:

Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression (By similarity).

Subcellular Location:

Cytoplasm. Cytoplasm>Cytoskeleton. Nucleus. Cell junction>Focal adhesion.
Note: Associates with the actin cytoskeleton near the adhesion plaques. Enters the nucleus in the presence of HESX1.

Subunit Structure:

Interacts with HPV type 6 protein E6. Does not interact significantly with E6 proteins from HPV types 11, 16, or 18. Interacts, via the Pro-rich regions, with the EVH1 domains of ENAH, EVL and VASP. Interacts with the first LIM domain of TES. Interacts with NEBL (isoform 2). Interacts with SYNPO2.

(Microbial infection) Interacts with human papillomavirus type 6/HPV6 protein E6. Does not interact significantly with E6 proteins from HPV types 11, 16, or 18.

Family&Domains:

Belongs to the zyxin/ajuba family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)

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