AFfirm™ CD46 Mouse Monoclonal Antibody - #BF8075
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Fold/Unfold
AHUS2; Antigen defined by monoclonal antibody TRA 2 10; Antigen identified by monoclonal antibody TRA 2 10; CD46; CD46 antigen; CD46 antigen complement regulatory protein; CD46 molecule; CD46 molecule complement regulatory protein; Complement membrane cofactor protein; MCP; MCP_HUMAN; Measles virus receptor; membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen); Membrane cofactor protein; MGC26544; MIC10; TLX; TRA2.10; Trophoblast leucocyte common antigen; Trophoblast leukocyte common antigen; Trophoblast lymphocyte cross reactive antigen;
Immunogens
- P15529 MCP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEPPGRRECPFPSWRFPGLLLAAMVLLLYSFSDACEEPPTFEAMELIGKPKPYYEIGERVDYKCKKGYFYIPPLATHTICDRNHTWLPVSDDACYRETCPYIRDPLNGQAVPANGTYEFGYQMHFICNEGYYLIGEEILYCELKGSVAIWSGKPPICEKVLCTPPPKIKNGKHTFSEVEVFEYLDAVTYSCDPAPGPDPFSLIGESTIYCGDNSVWSRAAPECKVVKCRFPVVENGKQISGFGKKFYYKATVMFECDKGFYLDGSDTIVCDSNSTWDPPVPKCLKVLPPSSTKPPALSHSVSTSSTTKSPASSASGPRPTYKPPVSNYPGYPKPEEGILDSLDVWVIAVIVIAIVVGVAVICVVPYRYLQRRKKKGTYLTDETHREVKFTSL
PTMs - P15529 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K51 | Ubiquitination | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| K153 | Ubiquitination | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| K167 | Ubiquitination | Uniprot | |
| K224 | Ubiquitination | Uniprot | |
| K237 | Ubiquitination | Uniprot | |
| T303 | Phosphorylation | Uniprot | |
| T320 | Phosphorylation | Uniprot | |
| K375 | Ubiquitination | Uniprot | |
| Y378 | Phosphorylation | Uniprot | |
| K388 | Ubiquitination | Uniprot | |
| S391 | Phosphorylation | Uniprot |
Research Backgrounds
Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity.
(Microbial infection) A number of viral and bacterial pathogens seem to bind MCP in order to exploit its immune regulation property and directly induce an immunosuppressive phenotype in T-cells.
(Microbial infection) Acts as a receptor for Adenovirus subgroup B2 and Ad3.
(Microbial infection) Acts as a receptor for cultured Measles virus.
(Microbial infection) Acts as a receptor for Herpesvirus 6/HHV-6.
(Microbial infection) May act as a receptor for pathogenic bacteria Neisseria and Streptococcus pyogenes.
N-glycosylated on Asn-83; Asn-114 and Asn-273 in most tissues, but probably less N-glycosylated in testis. N-glycosylation on Asn-114 and Asn-273 is required for cytoprotective function. N-glycosylation on Asn-114 is required for Measles virus binding. N-glycosylation on Asn-273 is required for Neisseria binding. N-glycosylation is not required for human adenovirus binding.
Extensively O-glycosylated in the Ser/Thr-rich domain. O-glycosylation is required for Neisseria binding but not for Measles virus or human adenovirus binding.
In epithelial cells, isoforms B/D/F/H/J/L/3 are phosphorylated by YES1 in response to infection by Neisseria gonorrhoeae; which promotes infectivity. In T-cells, these isoforms may be phosphorylated by LCK.
Cytoplasmic vesicle>Secretory vesicle>Acrosome inner membrane>Single-pass type I membrane protein.
Note: Inner acrosomal membrane of spermatozoa. Internalized upon binding of Measles virus, Herpesvirus 6 or Neisseria gonorrhoeae, which results in an increased susceptibility of infected cells to complement-mediated injury. In cancer cells or cells infected by Neisseria, shedding leads to a soluble peptide.
Expressed by all cells except erythrocytes.
Interacts with C3b. Interacts with C4b. Interacts with moesin/MSN.
(Microbial infection) Interacts with measles virus H protein.
(Microbial infection) Interacts with human herpesvirus 6 GH protein.
(Microbial infection) Interacts with human adenovirus B/D fiber protein.
(Microbial infection) Binds to Streptococcus pyogenes M protein and to type IV pili from Neisseria.
Sushi domains 1 and 2 are required for interaction with human adenovirus B PIV/FIBER protein and with Measles virus H protein. Sushi domains 2 and 3 are required for Herpesvirus 6 binding. Sushi domain 3 is required for Neisseria binding. Sushi domains 3 and 4 are required for interaction with Streptococcus pyogenes M protein and are the most important for interaction with C3b and C4b.
Research Fields
· Human Diseases > Infectious diseases: Viral > Measles.
· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)
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