Product: CD46 Mouse Monoclonal Antibody
Catalog: BF8075
Description: Mouse monoclonal antibody to CD46
Application: WB
Reactivity: Human, Rat
Prediction: Sheep
Mol.Wt.: 43kDa, 55 kDa; 44kD(Calculated).
Uniprot: P15529

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Product Info

Source:
Mouse
Application:
WB 1:1000-1:10000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Clonality:
Monoclonal [AFfirm8075]
Specificity:
CD46 Antibody detects endogenous levels of total CD46.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AHUS2; Antigen defined by monoclonal antibody TRA 2 10; Antigen identified by monoclonal antibody TRA 2 10; CD46; CD46 antigen; CD46 antigen complement regulatory protein; CD46 molecule; CD46 molecule complement regulatory protein; Complement membrane cofactor protein; MCP; MCP_HUMAN; Measles virus receptor; membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen); Membrane cofactor protein; MGC26544; MIC10; TLX; TRA2.10; Trophoblast leucocyte common antigen; Trophoblast leukocyte common antigen; Trophoblast lymphocyte cross reactive antigen;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P15529 MCP_HUMAN:

Expressed by all cells except erythrocytes.

Description:
CD46 is a type I membrane protein and a regulatory part of the complement system. This protein has cofactor activity for inactivation of complement components C3b and C4b by serum factor I, which protects the host cell from damage by complement. In addition, CD46 can act as a receptor for the Edmonston strain of measles virus, human herpesvirus-6, and type IV pili of pathogenic Neisseria. Finally, this protein may be involved in the fusion of the spermatozoa with the oocyte during fertilization. CD46 is found in a cluster on chromosome 1q32 with other proteins that are structural components of the complement system.
Sequence:
MEPPGRRECPFPSWRFPGLLLAAMVLLLYSFSDACEEPPTFEAMELIGKPKPYYEIGERVDYKCKKGYFYIPPLATHTICDRNHTWLPVSDDACYRETCPYIRDPLNGQAVPANGTYEFGYQMHFICNEGYYLIGEEILYCELKGSVAIWSGKPPICEKVLCTPPPKIKNGKHTFSEVEVFEYLDAVTYSCDPAPGPDPFSLIGESTIYCGDNSVWSRAAPECKVVKCRFPVVENGKQISGFGKKFYYKATVMFECDKGFYLDGSDTIVCDSNSTWDPPVPKCLKVLPPSSTKPPALSHSVSTSSTTKSPASSASGPRPTYKPPVSNYPGYPKPEEGILDSLDVWVIAVIVIAIVVGVAVICVVPYRYLQRRKKKGTYLTDETHREVKFTSL

PTMs - P15529 As Substrate

Site PTM Type Enzyme
K51 Ubiquitination
K66 Ubiquitination
K153 Ubiquitination
K159 Ubiquitination
K167 Ubiquitination
K224 Ubiquitination
K237 Ubiquitination
T303 Phosphorylation
T320 Phosphorylation
K375 Ubiquitination
Y378 Phosphorylation
K388 Ubiquitination
S391 Phosphorylation

Research Backgrounds

Function:

Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity.

(Microbial infection) A number of viral and bacterial pathogens seem to bind MCP in order to exploit its immune regulation property and directly induce an immunosuppressive phenotype in T-cells.

(Microbial infection) Acts as a receptor for Adenovirus subgroup B2 and Ad3.

(Microbial infection) Acts as a receptor for cultured Measles virus.

(Microbial infection) Acts as a receptor for Herpesvirus 6/HHV-6.

(Microbial infection) May act as a receptor for pathogenic bacteria Neisseria and Streptococcus pyogenes.

PTMs:

N-glycosylated on Asn-83; Asn-114 and Asn-273 in most tissues, but probably less N-glycosylated in testis. N-glycosylation on Asn-114 and Asn-273 is required for cytoprotective function. N-glycosylation on Asn-114 is required for Measles virus binding. N-glycosylation on Asn-273 is required for Neisseria binding. N-glycosylation is not required for human adenovirus binding.

Extensively O-glycosylated in the Ser/Thr-rich domain. O-glycosylation is required for Neisseria binding but not for Measles virus or human adenovirus binding.

In epithelial cells, isoforms B/D/F/H/J/L/3 are phosphorylated by YES1 in response to infection by Neisseria gonorrhoeae; which promotes infectivity. In T-cells, these isoforms may be phosphorylated by LCK.

Subcellular Location:

Cytoplasmic vesicle>Secretory vesicle>Acrosome inner membrane>Single-pass type I membrane protein.
Note: Inner acrosomal membrane of spermatozoa. Internalized upon binding of Measles virus, Herpesvirus 6 or Neisseria gonorrhoeae, which results in an increased susceptibility of infected cells to complement-mediated injury. In cancer cells or cells infected by Neisseria, shedding leads to a soluble peptide.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed by all cells except erythrocytes.

Subunit Structure:

Interacts with C3b. Interacts with C4b. Interacts with moesin/MSN.

(Microbial infection) Interacts with measles virus H protein.

(Microbial infection) Interacts with human herpesvirus 6 GH protein.

(Microbial infection) Interacts with human adenovirus B/D fiber protein.

(Microbial infection) Binds to Streptococcus pyogenes M protein and to type IV pili from Neisseria.

Family&Domains:

Sushi domains 1 and 2 are required for interaction with human adenovirus B PIV/FIBER protein and with Measles virus H protein. Sushi domains 2 and 3 are required for Herpesvirus 6 binding. Sushi domain 3 is required for Neisseria binding. Sushi domains 3 and 4 are required for interaction with Streptococcus pyogenes M protein and are the most important for interaction with C3b and C4b.

Research Fields

· Human Diseases > Infectious diseases: Viral > Measles.

· Organismal Systems > Immune system > Complement and coagulation cascades.   (View pathway)

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