OLR1 Mouse Monoclonal Antibody | Affinity Biosciences

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Western blot analysis of extracts from various samples, using OLR1 Mouse Monoclonal Antibody.

Product:	OLR1 Mouse Monoclonal Antibody
Catalog:	BF8072
Description:	Mouse monoclonal antibody to OLR1
Application:	WB
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	30~55kDa; 31kD(Calculated).
Uniprot:	P78380

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:1000-1:10000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Monoclonal [AFfirm8072]

Specificity:

OLR1 Antibody detects endogenous levels of total OLR1.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

C-type lectin domain family 8 member A; CLEC8A; hLOX 1; hLOX-1; Lectin like oxidized LDL receptor 1; Lectin like oxLDL receptor 1; Lectin type oxidized LDL receptor 1; Lectin-like oxidized LDL receptor 1; Lectin-like oxLDL receptor 1; Lectin-type oxidized LDL receptor 1; low density lipoprotein oxidized, receptor 1; LOX-1; LOXIN; Olr1; OLR1_HUMAN; Ox LDL receptor 1; Ox-LDL receptor 1; Oxidised low density lipoprotein (lectin like) receptor 1; Oxidized low density lipoprotein receptor 1; Oxidized low density lipoprotein receptor 1 soluble form; Oxidized low-density lipoprotein receptor 1; SCARE1; Scavenger receptor class E, member 1; SLOX1; soluble form; SR-EI;

Immunogens

Immunogen:

Uniprot:

P78380(OLR1_HUMAN) >>Visit HPA database.

Gene(ID):

OLR1(4973)

Expression:

P78380 OLR1_HUMAN:

Expressed at high level in endothelial cells and vascular-rich organs such as placenta, lung, liver and brain, aortic intima, bone marrow, spinal cord and substantia nigra. Also expressed at the surface of dendritic cells. Widely expressed at intermediate and low level.

Description:

This gene encodes a low density lipoprotein receptor that belongs to the C-type lectin superfamily. This gene is regulated through the cyclic AMP signaling pathway. The encoded protein binds, internalizes and degrades oxidized low-density lipoprotein. This protein may be involved in the regulation of Fas-induced apoptosis. This protein may play a role as a scavenger receptor. Mutations of this gene have been associated with atherosclerosis, risk of myocardial infarction, and may modify the risk of Alzheimer's disease. Alternate splicing results in multiple transcript variants.

Sequence:

P78380 OLR1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTFDDLKIQTVKDQPDEKSNGKKAKGLQFLYSPWWCLAAATLGVLCLGLVVTIMVLGMQLSQVSDLLTQEQANLTHQKKKLEGQISARQQAEEASQESENELKEMIETLARKLNEKSKEQMELHHQNLNLQETLKRVANCSAPCPQDWIWHGENCYLFSSGSFNWEKSQEKCLSLDAKLLKINSTADLDFIQQAISYSSFPFWMGLSRRNPSYPWLWEDGSPLMPHLFRVRGAVSQTYPSGTCAYIQRGAVYAENCILAAFSICQKKANLRAQ

PTMs - P78380 As Substrate

P78380

Site	PTM Type	Source
K7	Ubiquitination	Uniprot
K12	Ubiquitination	Uniprot
S19	Phosphorylation	Uniprot
K80	Ubiquitination	Uniprot
S95	Phosphorylation	Uniprot
S98	Phosphorylation	Uniprot
K112	Ubiquitination	Uniprot
K118	Ubiquitination	Uniprot
N139	N-Glycosylation	Uniprot
K171	Ubiquitination	Uniprot
K178	Ubiquitination	Uniprot

Research Backgrounds

P78380_OLR1_HUMAN

Function:

Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria.

PTMs:

The intrachain disulfide-bonds prevent N-glycosylation at some sites.

N-glycosylated.

Subcellular Location:

Cell membrane>Lipid-anchor. Cell membrane>Single-pass type II membrane protein. Membrane raft. Secreted.
Note: A secreted form also exists. Localization to membrane rafts requires palmitoylation.

Tissue Specificity:

Subunit Structure:

Homodimer; disulfide-linked. May form a hexamer composed of 3 homodimers. Interacts with HSP70.

Family&Domains:

The cytoplasmic region is required for subcellular sorting on the cell surface.

The C-type lectin domain mediates the recognition and binding of oxLDL.

Research Fields

· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)

· Organismal Systems > Endocrine system > PPAR signaling pathway.

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AFfirm™ OLR1 Mouse Monoclonal Antibody - #BF8072