PTHR1 Mouse Monoclonal Antibody | Affinity Biosciences

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Western blot analysis of extracts from various samples, using PTHR1 Mouse Monoclonal Antibody.

Product:	PTHR1 Mouse Monoclonal Antibody
Catalog:	BF8066
Description:	Mouse monoclonal antibody to PTHR1
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	70KD; 66kD(Calculated).
Uniprot:	Q03431

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Mouse

Application:

WB 1:1000-1:10000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Monoclonal [AFfirm8066]

Specificity:

PTHR1 Antibody detects endogenous levels of total PTHR1.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

MGC138426; MGC138452; Parathyroid hormone 1 receptor; Parathyroid hormone/parathyroid hormone related peptide receptor; Parathyroid hormone/parathyroid hormone related protein receptor; Parathyroid hormone/parathyroid hormone-related peptide receptor; PTH receptor; PTH/PTHr receptor; PTH/PTHrP receptor; PTH/PTHrP type I receptor; PTH1 receptor; PTH1R; PTH1R_HUMAN; PTHR 1; PTHR; PTHR1;

Immunogens

Immunogen:

Uniprot:

Q03431(PTH1R_HUMAN) >>Visit HPA database.

Gene(ID):

PTH1R(5745)

Expression:

Q03431 PTH1R_HUMAN:

Expressed in most tissues. Most abundant in kidney, bone and liver.

Sequence:

Q03431 PTH1R_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGTARIAPGLALLLCCPVLSSAYALVDADDVMTKEEQIFLLHRAQAQCEKRLKEVLQRPASIMESDKGWTSASTSGKPRKDKASGKLYPESEEDKEAPTGSRYRGRPCLPEWDHILCWPLGAPGEVVAVPCPDYIYDFNHKGHAYRRCDRNGSWELVPGHNRTWANYSECVKFLTNETREREVFDRLGMIYTVGYSVSLASLTVAVLILAYFRRLHCTRNYIHMHLFLSFMLRAVSIFVKDAVLYSGATLDEAERLTEEELRAIAQAPPPPATAAAGYAGCRVAVTFFLYFLATNYYWILVEGLYLHSLIFMAFFSEKKYLWGFTVFGWGLPAVFVAVWVSVRATLANTGCWDLSSGNKKWIIQVPILASIVLNFILFINIVRVLATKLRETNAGRCDTRQQYRKLLKSTLVLMPLFGVHYIVFMATPYTEVSGTLWQVQMHYEMLFNSFQGFFVAIIYCFCNGEVQAEIKKSWSRWTLALDFKRKARSGSSSYSYGPMVSHTSVTNVGPRVGLGLPLSPRLLPTATTNGHPQLPGHAKPGTPALETLETTPPAMAAPKDDGFLNGSCSGLDEEASGPERPPALLQEEWETVM

PTMs - Q03431 As Substrate

Q03431

Site	PTM Type	Source
Y23	Phosphorylation	Uniprot
S73	Phosphorylation	Uniprot
S91	Phosphorylation	Uniprot
T99	Phosphorylation	Uniprot
S101	Phosphorylation	Uniprot
T192	Phosphorylation	Uniprot
Y320	Phosphorylation	Uniprot
T387	Phosphorylation	Uniprot
K388	Ubiquitination	Uniprot
T392	Phosphorylation	Uniprot
S473	Phosphorylation	Uniprot
K484	Ubiquitination	Uniprot
S489	Phosphorylation	Uniprot
S491	Phosphorylation	Uniprot
S492	Phosphorylation	Uniprot
S493	Phosphorylation	Uniprot
Y494	Phosphorylation	Uniprot
S495	Phosphorylation	Uniprot
Y496	Phosphorylation	Uniprot
S501	Phosphorylation	Uniprot
T503	Phosphorylation	Uniprot
S504	Phosphorylation	Uniprot
S519	Phosphorylation	Uniprot
T547	Phosphorylation	Uniprot
T551	Phosphorylation	Uniprot
T591	Phosphorylation	Uniprot

Research Backgrounds

Q03431_PTH1R_HUMAN

Function:

Receptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system.

PTMs:

N-glycosylated.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Tissue Specificity:

Expressed in most tissues. Most abundant in kidney, bone and liver.

Subunit Structure:

Interacts (via N-terminal extracellular domain) with PTHLH and PTH. Homodimer in the absence of bound ligand. Peptide hormone binding leads to dissociation of the homodimer.

Family&Domains:

Belongs to the G-protein coupled receptor 2 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Excretory system > Endocrine and other factor-regulated calcium reabsorption.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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AFfirm™ PTHR1 Mouse Monoclonal Antibody - #BF8066