Product: hnRNP A1 Mouse Monoclonal Antibody
Catalog: BF8054
Description: Mouse monoclonal antibody to hnRNP A1
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 33 KD; 39kD(Calculated).
Uniprot: P09651

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Mouse
Application:
IHC 1:50-1:200, WB 1:1000-1:10000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Monoclonal [AFfirm8054]
Specificity:
hnRNP A1 Antibody detects endogenous levels of total hnRNP A1.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

HNRNPA 1; Helix destabilizing protein; Helix-destabilizing protein; Heterogeneous nuclear ribonucleoprotein A1; Heterogeneous nuclear ribonucleoprotein A1B protein; Heterogeneous nuclear ribonucleoprotein B2 protein; Heterogeneous nuclear ribonucleoprotein core protein A1; hnRNP A1; hnRNP core protein A1; HNRNPA1; HNRPA1; MGC102835; Nuclear ribonucleoprotein particle A1 protein; ROA1_HUMAN; Single strand DNA binding protein UP1; Single strand RNA binding protein; Single-strand RNA-binding protein;

Immunogens

Immunogen:

A synthesized peptide derived from human hnRNP A1, corresponding to a region within the internal amino acids.

Uniprot:
Gene(ID):
Sequence:
MSKSESPKEPEQLRKLFIGGLSFETTDESLRSHFEQWGTLTDCVVMRDPNTKRSRGFGFVTYATVEEVDAAMNARPHKVDGRVVEPKRAVSREDSQRPGAHLTVKKIFVGGIKEDTEEHHLRDYFEQYGKIEVIEIMTDRGSGKKRGFAFVTFDDHDSVDKIVIQKYHTVNGHNCEVRKALSKQEMASASSSQRGRSGSGNFGGGRGGGFGGNDNFGRGGNFSGRGGFGGSRGGGGYGGSGDGYNGFGNDGGYGGGGPGYSGGSRGYGSGGQGYGNQGSGYGGSGSYDSYNNGGGGGFGGGSGSNFGGGGSYNDFGNYNNQSSNFGPMKGGNFGGRSSGPYGGGGQYFAKPRNQGGYGGSSSSSSYGSGRRF

PTMs - P09651 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S2 Acetylation
S2 Phosphorylation
K3 Acetylation
K3 Ubiquitination
S4 Phosphorylation Q9UBS0 (RPS6KB2)
S6 Phosphorylation Q9UBS0 (RPS6KB2)
K8 Acetylation
K8 Ubiquitination
R14 Methylation
K15 Acetylation
K15 Sumoylation
K15 Ubiquitination
S22 Phosphorylation
T25 Phosphorylation
S29 Phosphorylation
R31 Methylation
C43 S-Nitrosylation
K52 Acetylation
K52 Ubiquitination
R55 Methylation
T61 Phosphorylation
Y62 Phosphorylation
K78 Ubiquitination
K87 Acetylation
K87 Ubiquitination
S91 Phosphorylation
S95 Phosphorylation P17252 (PRKCA)
T103 Phosphorylation
K105 Acetylation
K105 Ubiquitination
K106 Methylation
K106 Ubiquitination
K113 Acetylation
K113 Sumoylation
K113 Ubiquitination
R122 Methylation
Y124 Phosphorylation
Y128 Phosphorylation
T138 Phosphorylation
R140 Methylation
S142 Phosphorylation
K144 Acetylation
K144 Ubiquitination
K145 Acetylation
R146 Methylation
S158 Phosphorylation
K161 Methylation
K161 Ubiquitination
K166 Acetylation
K166 Ubiquitination
Y167 Phosphorylation
T169 Phosphorylation
C175 S-Nitrosylation
K183 Sumoylation
K183 Ubiquitination
S190 Phosphorylation
S191 Phosphorylation
S192 Phosphorylation Q9BUB5 (MKNK1)
R194 Methylation
R196 Methylation
S197 Phosphorylation
S199 Phosphorylation P17612 (PRKACA) , P68400 (CSNK2A1) , P31749 (AKT1)
R206 Methylation
R218 Methylation
R225 Methylation
R232 Methylation
Y244 Phosphorylation
Y253 Phosphorylation
R265 Methylation
Y312 Phosphorylation
Y318 Phosphorylation
K329 Sumoylation
R336 Methylation
S337 Phosphorylation
S338 Phosphorylation
Y341 Phosphorylation
Y347 Phosphorylation
K350 Acetylation
K350 Methylation
K350 Sumoylation
K350 Ubiquitination
R352 Methylation
Y357 Phosphorylation
S360 Phosphorylation
S361 Phosphorylation
S362 Phosphorylation Q9BUB5 (MKNK1)
S363 Phosphorylation Q9BUB5 (MKNK1)
S364 Phosphorylation Q9BUB5 (MKNK1)
S365 Phosphorylation
Y366 Phosphorylation
S368 Phosphorylation
R370 Methylation
R371 Methylation

Research Backgrounds

Function:

Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May bind to specific miRNA hairpins. Binds to the IRES and thereby inhibits the translation of the apoptosis protease activating factor APAF1.

(Microbial infection) May play a role in HCV RNA replication.

(Microbial infection) Cleavage by Enterovirus 71 protease 3C results in increased translation of apoptosis protease activating factor APAF1, leading to apoptosis.

PTMs:

Arg-194, Arg-206 and Arg-225 are dimethylated, probably to asymmetric dimethylarginine.

Sumoylated.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes (PubMed:17289661).

Cytoplasm.
Note: (Microbial infection) In the course of viral infection, colocalizes with HCV NS5B at speckles in the cytoplasm in a HCV-replication dependent manner.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Identified in the spliceosome C complex. Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with SEPT6. Interacts with C9orf72. Interacts with KHDRBS1. Interacts with UBQLN2.

(Microbial infection) Interacts with HCV NS5B and with the 5'-UTR and 3'-UTR of HCV RNA.

Research Fields

· Genetic Information Processing > Transcription > Spliceosome.

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