AFfirm™ XRCC1 Mouse Monoclonal Antibody - #BF8039

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Product: XRCC1 Mouse Monoclonal Antibody
Catalog: BF8039
Description: Mouse monoclonal antibody to XRCC1
Application: WB
Reactivity: Human, Mouse
Prediction: Horse, Sheep, Rabbit, Dog
Mol.Wt.: 90kDa; 69kD(Calculated).
Uniprot: P18887

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Monoclonal [AFfirm8039(AFP21835)]
Specificity:
XRCC1 Antibody detects endogenous levels of total XRCC1.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DNA repair protein XRCC1; RCC; X ray repair complementing defective repair in chinese hamster; X ray Repair Complementing Defective Repair in Chinese Hamster Cells; X ray repair complementing defective repair in chinese hamster cells 1; X ray repair cross complementing 1; X ray repair cross complementing protein 1; X ray repair, complementing defective, repair in Chinese hamster; X-ray repair cross-complementing protein 1; XRCC 1; Xrcc1; XRCC1_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human XRCC1, corresponding to a region within the internal amino acids.

Uniprot:

P18887(XRCC1_HUMAN) >>Visit HPA database.

Gene(ID):
XRCC1(7515)
Expression:
P18887 XRCC1_HUMAN:

Expressed in fibroblasts, retinal pigmented epithelial cells and lymphoblastoid cells (at protein level).

Description:
XRCC1 Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Homodimer. Interacts with polynucleotide kinase (PNK), DNA polymerase-beta (POLB) and DNA ligase III (LIG3).
Sequence:
MPEIRLRHVVSCSSQDSTHCAENLLKADTYRKWRAAKAGEKTISVVLQLEKEEQIHSVDIGNDGSAFVEVLVGSSAGGAGEQDYEVLLVTSSFMSPSESRSGSNPNRVRMFGPDKLVRAAAEKRWDRVKIVCSQPYSKDSPFGLSFVRFHSPPDKDEAEAPSQKVTVTKLGQFRVKEEDESANSLRPGALFFSRINKTSPVTASDPAGPSYAAATLQASSAASSASPVSRAIGSTSKPQESPKGKRKLDLNQEEKKTPSKPPAQLSPSVPKRPKLPAPTRTPATAPVPARAQGAVTGKPRGEGTEPRRPRAGPEELGKILQGVVVVLSGFQNPFRSELRDKALELGAKYRPDWTRDSTHLICAFANTPKYSQVLGLGGRIVRKEWVLDCHRMRRRLPSRRYLMAGPGSSSEEDEASHSGGSGDEAPKLPQKQPQTKTKPTQAAGPSSPQKPPTPEETKAASPVLQEDIDIEGVQSEGQDNGAEDSGDTEDELRRVAEQKEHRLPPGQEENGEDPYAGSTDENTDSEEHQEPPDLPVPELPDFFQGKHFFLYGEFPGDERRKLIRYVTAFNGELEDNMSDRVQFVITAQEWDPSFEEALMDNPSLAFVRPRWIYSCNEKQKLLPHQLYGVVPQA

PTMs - P18887 As Substrate

Site PTM Type Enzyme
K129 Ubiquitination
K138 Ubiquitination
S140 Phosphorylation
S151 Phosphorylation
S162 Phosphorylation
K169 Acetylation
K169 Ubiquitination
K176 Sumoylation
S184 Phosphorylation O96017 (CHEK2)
T198 Phosphorylation
S199 Phosphorylation
T202 Phosphorylation
S204 Phosphorylation
S210 Phosphorylation O96017 (CHEK2)
Y211 Phosphorylation
S224 Phosphorylation
S226 Phosphorylation P24941 (CDK2)
S229 Phosphorylation P24941 (CDK2)
S234 Phosphorylation
T235 Phosphorylation
S236 Phosphorylation
S241 Phosphorylation
K247 Sumoylation
K256 Acetylation
K256 Sumoylation
T257 Phosphorylation
S259 Phosphorylation
K260 Acetylation
S266 Phosphorylation
S268 Phosphorylation
K271 Acetylation
T279 Phosphorylation
T281 Phosphorylation
T284 Phosphorylation O96017 (CHEK2)
T296 O-Glycosylation
T296 Phosphorylation
K298 Acetylation
R310 Methylation
K341 Ubiquitination
K348 Ubiquitination
S357 Phosphorylation
T358 Phosphorylation
T367 Phosphorylation
Y370 Phosphorylation
S371 Phosphorylation P78527 (PRKDC)
K383 Ubiquitination
S408 Phosphorylation P68400 (CSNK2A1)
S409 Phosphorylation P68400 (CSNK2A1)
S410 Phosphorylation P68400 (CSNK2A1)
S416 Phosphorylation P68400 (CSNK2A1)
S418 Phosphorylation P68400 (CSNK2A1)
S421 Phosphorylation P68400 (CSNK2A1)
K431 Ubiquitination
T437 Phosphorylation
T440 O-Glycosylation
T440 Phosphorylation
S446 O-Glycosylation
S446 Phosphorylation
S447 O-Glycosylation
S447 Phosphorylation
T453 O-Glycosylation
T453 Phosphorylation
T457 Phosphorylation
S461 Phosphorylation P68400 (CSNK2A1)
S475 Phosphorylation P68400 (CSNK2A1)
S485 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2)
T488 Phosphorylation P19784 (CSNK2A2) , P68400 (CSNK2A1)
Y515 Phosphorylation P68400 (CSNK2A1)
S518 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2)
T519 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2)
T523 Phosphorylation P19784 (CSNK2A2) , P68400 (CSNK2A1)
S525 Phosphorylation P68400 (CSNK2A1)
S578 Phosphorylation P68400 (CSNK2A1)
K620 Ubiquitination
Y627 Phosphorylation

Research Backgrounds

Function:

Involved in DNA single-strand break repair by mediating the assembly of DNA break repair protein complexes. Probably during DNA repair, negatively regulates ADP-ribose levels by modulating ADP-ribosyltransferase PARP1 activity.

PTMs:

Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.

Sumoylated.

Subcellular Location:

Nucleus.
Note: Moves from the nucleoli to the global nuclear chromatin upon DNA damage.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in fibroblasts, retinal pigmented epithelial cells and lymphoblastoid cells (at protein level).

Subunit Structure:

Homodimer. Interacts with polynucleotide kinase (PNK), DNA polymerase-beta (POLB) and DNA ligase III (LIG3). Interacts with APTX and APLF. Interacts with APEX1; the interaction is induced by SIRT1 and increases with the acetylated form of APEX1.

Research Fields

· Genetic Information Processing > Replication and repair > Base excision repair.

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