WB

RT

【Product Introduction】 Western Blotting Antibody Stripping Buffer is an enhanced formulation for removing bound primary and secondary antibodies on membranes, allowing users to re-detect membranes with chemiluminescent substrates. 【Product features】 1. Ready-to-use, simple operation - no need to dilute; No unpleasant odors; Store at room temperature. 2. Compatibility - can be used on wet or dry NC membranes and PVDF membranes; Can be used with any blocking buffer, enzyme conjugate, and chemiluminescent substrate. 3. Cost-effective - save valuable time and samples; Effective peeling on first use. 4. Strong and gentle - effectively retains the transferred protein, and multiple antibody peeling operations can be performed on the blotting. 5. Flexible—used to strip and re-detect antibodies to optimize antibody concentration, or to detect new antigens with different antibodies. 【Usage】 1. Rinse the blot for 5 min on TBST to remove chemiluminescent substrate. 2. Add enough antibody peeling buffer to completely cover the imprinted membrane, and incubate at 37°C with gentle shaking for 20~35 minutes. 3. Remove the blot membrane and gently shake and dip in TBST for 5 min x2 times. 4. Closed blot film. 5. Proceed to the next blot experiment.