AFfirm™ Cytokeratin 4 Mouse Monoclonal Antibody - #BF8030

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Product: Cytokeratin 4 Mouse Monoclonal Antibody
Catalog: BF8030
Description: Mouse monoclonal antibody to Cytokeratin 4
Application: WB
Reactivity: Human
Mol.Wt.: 56kDa; 56kD(Calculated).
Uniprot: P19013

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 50ul $250 In stock
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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse IgG1
Application:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [AFfirm8030(AFP21818)]
Specificity:
Cytokeratin 4 Mouse monoclonal antibody detects endogenous levels of total Cytokeratin 4.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CK 4; CK-4; CK4; CYK4; Cytokeratin 4; Cytokeratin-4; Cytokeratin4; FLJ31692; K2C4_HUMAN; K4; Keratin 4; Keratin; Keratin type II cytoskeletal 4; Keratin-4; Keratin4; KRT 4; Krt4; type II cytoskeletal 4; Type-II keratin Kb4;

Immunogens

Immunogen:

A synthesized peptide derived from Human Cytokeratin 4.

Uniprot:

P19013(K2C4_HUMAN) >>Visit HPA database.

Gene(ID):
KRT4(3851)
Expression:
P19013 K2C4_HUMAN:

Detected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa (at protein level). Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer.

Sequence:
MIARQQCVRGGPRGFSCGSAIVGGGKRGAFSSVSMSGGAGRCSSGGFGSRSLYNLRGNKSISMSVAGSRQGACFGGAGGFGTGGFGGGFGGSFSGKGGPGFPVCPAGGIQEVTINQSLLTPLHVEIDPEIQKVRTEEREQIKLLNNKFASFIDKVQFLEQQNKVLETKWNLLQQQTTTTSSKNLEPLFETYLSVLRKQLDTLGNDKGRLQSELKTMQDSVEDFKTKYEEEINKRTAAENDFVVLKKDVDAAYLNKVELEAKVDSLNDEINFLKVLYDAELSQMQTHVSDTSVVLSMDNNRNLDLDSIIAEVRAQYEEIAQRSKAEAEALYQTKVQQLQISVDQHGDNLKNTKSEIAELNRMIQRLRAEIENIKKQCQTLQVSVADAEQRGENALKDAHSKRVELEAALQQAKEELARMLREYQELMSVKLALDIEIATYRKLLEGEEYRMSGECQSAVSISVVSGSTSTGGISGGLGSGSGFGLSSGFGSGSGSGFGFGGSVSGSSSSKIISTTTLNKRR

PTMs - P19013 As Substrate

Site PTM Type Enzyme
S16 Phosphorylation
S19 Phosphorylation
K26 Acetylation
S31 Phosphorylation
S32 Phosphorylation
S34 Phosphorylation
S36 Phosphorylation
S49 Phosphorylation
S51 Phosphorylation
Y53 Phosphorylation
S60 Phosphorylation
S64 Phosphorylation
S68 Phosphorylation
K161 Ubiquitination
S164 Phosphorylation
K168 Ubiquitination
Y241 Phosphorylation
K259 Acetylation
Y266 Phosphorylation
K269 Acetylation
Y329 Phosphorylation
Y344 Phosphorylation
T346 Phosphorylation
S413 Phosphorylation
Y436 Phosphorylation
S441 Phosphorylation
Y462 Phosphorylation

Research Backgrounds

Tissue Specificity:

Detected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa (at protein level). Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer.

Subunit Structure:

Heterotetramer of two type I and two type II keratins. Keratin-4 is generally associated with keratin-13.

Family&Domains:

Belongs to the intermediate filament family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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