AFfirm™ Nrf2 mouse monoclonal Antibody - #BF8017

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Product: Nrf2 mouse monoclonal Antibody
Catalog: BF8017
Description: Mouse monoclonal antibody to Nrf2
Application: WB IHC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Mol.Wt.: 100～120kD; 68kD(Calculated).
Uniprot: Q16236

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Mouse
Application:
WB 1:500-1:3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Monoclonal [AFfirm8017]
Specificity:
Nrf2 Antibody detects endogenous levels of total Nrf2.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

erythroid derived 2; HEBP1; like 2; NF E2 related factor 2; NF-E2-related factor 2; NF2L2_HUMAN; NFE2 related factor 2; NFE2-related factor 2; Nfe2l2; Nrf 2; NRF2; Nuclear factor (erythroid derived 2) like 2; Nuclear factor; nuclear factor erythroid 2 like 2; Nuclear factor erythroid 2 related factor 2; Nuclear factor erythroid 2-related factor 2; Nuclear factor erythroid derived 2 like 2;

Immunogens

Immunogen:

A synthesized peptide derived from human nrf2, corresponding to a region within the internal amino acids.

Uniprot:

Q16236(NF2L2_HUMAN) >>Visit HPA database.

Gene(ID):
NFE2L2(4780)
Expression:
Q16236 NF2L2_HUMAN:

Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.

Sequence:
MMDLELPPPGLPSQQDMDLIDILWRQDIDLGVSREVFDFSQRRKEYELEKQKKLEKERQEQLQKEQEKAFFAQLQLDEETGEFLPIQPAQHIQSETSGSANYSQVAHIPKSDALYFDDCMQLLAQTFPFVDDNEVSSATFQSLVPDIPGHIESPVFIATNQAQSPETSVAQVAPVDLDGMQQDIEQVWEELLSIPELQCLNIENDKLVETTMVPSPEAKLTEVDNYHFYSSIPSMEKEVGNCSPHFLNAFEDSFSSILSTEDPNQLTVNSLNSDATVNTDFGDEFYSAFIAEPSISNSMPSPATLSHSLSELLNGPIDVSDLSLCKAFNQNHPESTAEFNDSDSGISLNTSPSVASPEHSVESSSYGDTLLGLSDSEVEELDSAPGSVKQNGPKTPVHSSGDMVQPLSPSQGQSTHVHDAQCENTPEKELPVSPGHRKTPFTKDKHSSRLEAHLTRDELRAKALHIPFPVEKIINLPVVDFNEMMSKEQFNEAQLALIRDIRRRGKNKVAAQNCRKRKLENIVELEQDLDHLKDEKEKLLKEKGENDKSLHLLKKQLSTLYLEVFSMLRDEDGKPYSPSEYSLQQTRDGNVFLVPKSKKPDVKKN

Research Backgrounds

Function:

Transcription factor that plays a key role in the response to oxidative stress: binds to antioxidant response (ARE) elements present in the promoter region of many cytoprotective genes, such as phase 2 detoxifying enzymes, and promotes their expression, thereby neutralizing reactive electrophiles. In normal conditions, ubiquitinated and degraded in the cytoplasm by the BCR(KEAP1) complex. In response to oxidative stress, electrophile metabolites inhibit activity of the BCR(KEAP1) complex, promoting nuclear accumulation of NFE2L2/NRF2, heterodimerization with one of the small Maf proteins and binding to ARE elements of cytoprotective target genes. The NFE2L2/NRF2 pathway is also activated in response to selective autophagy: autophagy promotes interaction between KEAP1 and SQSTM1/p62 and subsequent inactivation of the BCR(KEAP1) complex, leading to NFE2L2/NRF2 nuclear accumulation and expression of cytoprotective genes. May also be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.

PTMs:

Ubiquitinated in the cytoplasm by the BCR(KEAP1) E3 ubiquitin ligase complex leading to its degradation. In response to oxidative stress, electrophile metabolites, such as sulforaphane, modify KEAP1, leading to inhibit activity of the BCR(KEAP1) complex, promoting NFE2L2/NRF2 nuclear accumulation and activity. In response to autophagy, the BCR(KEAP1) complex is inactivated (By similarity).

Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.

Acetylation at Lys-596 and Lys-599 increases nuclear localization whereas deacetylation by SIRT1 enhances cytoplasmic presence.

Glycation impairs transcription factor activity by preventing heterodimerization with small Maf proteins. Deglycation by FN3K restores activity.

Subcellular Location:

Cytoplasm>Cytosol. Nucleus.
Note: Cytosolic under unstressed conditions: ubiquitinated and degraded by the BCR(KEAP1) E3 ubiquitin ligase complex (PubMed:15601839, PubMed:21196497). Translocates into the nucleus upon induction by electrophilic agents that inactivate the BCR(KEAP1) E3 ubiquitin ligase complex (PubMed:21196497).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.

Family&Domains:

The ETGE motif, and to a lower extent the DLG motif, mediate interaction with KEAP1.

Belongs to the bZIP family. CNC subfamily.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

References

1). Supplementation of Clostridium butyricum Alleviates Vascular Inflammation in Diabetic Mice. Diabetes & metabolism journal, 2024 (PubMed: 38310882) [IF=6.8]
2). Accelerating diabetic wound healing with Ramulus Mori (Sangzhi) alkaloids via NRF2/HO-1/eNOS pathway. PHYTOMEDICINE, 2024 [IF=6.7]
3). Withaferin A inhibits ferroptosis and protects against intracerebral hemorrhage. Neural Regeneration Research, 2023 (PubMed: 36453416) [IF=5.9]

Application: WB    Species: Mouse    Sample:

Figure 6 WFA upregulates nuclear factor E2-related factor 2 (Nrf2) expression and promotes nuclear translocation of Nrf2 after ICH in vivo and in vitro. (A) Venn analysis of overlapping genes that interact with both WFA and HO-1 in the CTD database and String database. (B) Representative western blot images and (C) quantitative analysis of relative protein level of cytoplasmic Nrf2 and nuclear Nrf2 in the sham, ICH, and WFA (0.1, 1, and 5 μg/kg)-treated groups on day 7 after ICH. (D) Quantification of relative nuclear and cytoplasmic Nrf2 protein levels in the sham, ICH, and WFA (0.1/1/5 μg/kg)-treated groups on day 7 after ICH. (E) Representative western blot images and (F and G) quantification of relative protein expression of cytoplasmic Nrf2, nuclear Nrf2, and ration of nuclear/cytoplasmic Nrf2 in the hemin-induced ICH model with or without WFA treatment. (H) Nrf2 localization was detected by immunofluorescence with an anti-Nrf2 antibody (green fluorescence). Nuclei were stained with DAPI (blue fluorescence). (I) Allocation of green and blue signals in the dual-channel overlay images was analyzed with the ImageJ “Co-localization Finder” plugin, and co-localization of the green and blue signals is shown in white spots. The x-axes indicate the intensities of the green signals from the green channel (Nrf2) and the y-axes from the blue channel (DAPI). For every scatterplot, the intensities are given as the pixel grey values ranging from 0 to 255. Co-localization clusters the pixels from two channels along the diagonal. Pearson’s correlation coefficient (Rr) reflects the intensity distribution relationship between the two channels. Manders overlap coefficient (MOC) reflects the true degree of co-localization of the two channels. The maximal theoretical value for the Rr and MOC is 1.0. Quantification analysis of Nrf2 nuclear location with parameter. (J) Pearson’s correlation coefficient (Rr) and (K) MOC. n = 6 mice per group in B–D; n = 3 per group in E–K. *P < 0.05, **P < 0.01 (one-way analysis of variance followed by Bonferroni correction). Data are presented as the mean ± SEM. HO-1: Heme oxygenase-1; ICH: intracerebral hemorrhage; Nrf2: nuclear factor erythroid-related factor 2; WFA: withaferin A.
4). Antioxidation and Anti-Inflammatory Activity of Prussian Blue Nanozymes to Alleviate Acetaminophen-Induced Acute Liver Injury. ACS Applied Nano Materials, 2023 [IF=5.9]
5). Polysaccharide Isolated from Agaricus blazei Murill Alleviates Intestinal Ischemia/Reperfusion Injury through Regulating Gut Microbiota and Mitigating Inflammation in Mice. Journal of agricultural and food chemistry, 2024 (PubMed: 38247134) [IF=5.7]
6). Maf1 mitigates sevoflurane-induced microglial inflammatory damage and attenuates microglia-mediated neurotoxicity in HT-22 cells by activating the AMPK/Nrf2 signaling. NEUROTOXICOLOGY, 2022 (PubMed: 35430185) [IF=3.4]
7). FGF1 reduces cartilage injury in osteoarthritis via regulating AMPK/Nrf2 pathway. Journal of Molecular Histology, 2023 (PubMed: 37659992) [IF=2.9]

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