Product: MBP Mouse Monoclonal Antibody
Catalog: BF8010
Description: Mouse monoclonal antibody to MBP
Application: WB IHC
Reactivity: Mouse, Rat
Mol.Wt.: 33kDa; 33kD(Calculated).
Uniprot: P02686

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Product Info

Source:
Mouse
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Mouse,Rat
Clonality:
Monoclonal [AFfirm8010(AFB19414)]
Specificity:
MBP antibody detects endogenous levels of total MBP.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

GDB; Golli MBP; Golli MBP; myelin basic protein; Hemopoietic MBP; HMBPR; HUGO; MBP; MBP_CAVPO; MBP_HUMAN; MGC99675; MLD; Myelin A1 protein; Myelin A1 Protein, basic; Myelin basic protein; Myelin Deficient; Myelin membrane encephalitogenic protein; OTTHUMP00000163776; OTTHUMP00000174387; OTTHUMP00000174388; SHI; Shiverer; SP;

Immunogens

Immunogen:

A synthesized peptide derived from human MBP.

Uniprot:
Gene(ID):
Expression:
P02686 MBP_HUMAN:

MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.

Sequence:
MGNHAGKRELNAEKASTNSETNRGESEKKRNLGELSRTTSEDNEVFGEADANQNNGTSSQDTAVTDSKRTADPKNAWQDAHPADPGSRPHLIRLFSRDAPGREDNTFKDRPSESDELQTIQEDSAATSESLDVMASQKRPSQRHGSKYLATASTMDHARHGFLPRHRDTGILDSIGRFFGGDRGAPKRGSGKDSHHPARTAHYGSLPQKSHGRTQDENPVVHFFKNIVTPRTPPPSQGKGRGLSLSRFSWGAEGQRPGFGYGGRASDYKSAHKGFKGVDAQGTLSKIFKLGGRDSRSGSPMARR

PTMs - P02686 As Substrate

Site PTM Type Enzyme
S16 Phosphorylation
S19 Phosphorylation
S26 Phosphorylation
S36 Phosphorylation
T38 Phosphorylation
T39 Phosphorylation
S40 Phosphorylation
T65 Phosphorylation
S87 Phosphorylation
S96 Phosphorylation P27361 (MAPK3)
T106 Phosphorylation
S114 Phosphorylation
S141 Phosphorylation
S146 Phosphorylation P17612 (PRKACA)
Y148 Phosphorylation
S153 Phosphorylation
T154 Phosphorylation
T169 Phosphorylation P17612 (PRKACA)
S190 Phosphorylation P17612 (PRKACA)
Y203 Phosphorylation
S205 Phosphorylation
T229 Phosphorylation P28482 (MAPK1)
T232 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3)
R241 Methylation
S249 Phosphorylation P17612 (PRKACA)
Y261 Phosphorylation
S266 Phosphorylation P17612 (PRKACA)
T283 Phosphorylation
S285 Phosphorylation
S295 Phosphorylation P17612 (PRKACA)
S299 Phosphorylation Q8TAS1 (UHMK1)

Research Backgrounds

Function:

The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.

PTMs:

Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.

The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).

Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.

Phosphorylated by TAOK2, VRK2, MAPK11, MAPK12, MAPK14 and MINK1.

Subcellular Location:

Plasma membrane>Myelin membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Cytoplasmic side of myelin.

Nucleus.
Note: Targeted to nucleus in oligodendrocytes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.

Subunit Structure:

Homodimer. Isoform 3 exists as a homodimer.

Family&Domains:

Belongs to the myelin basic protein family.

References

1). Zhang D et al. RSV Infection in Neonatal Mice Induces Pulmonary Eosinophilia Responsible for Asthmatic Reaction. Frontiers in Immunology 2022 Feb 2;13:817113. (PubMed: 35185908) [IF=7.3]

2). Jia N et al. Staphylococcal superantigen-like protein 10 induces necroptosis through TNFR1 activation of RIPK3-dependent signal pathways. Communications Biology 2022 Aug 12;5(1):813. (PubMed: 35962126) [IF=5.9]

Application: WB    Species: Human    Sample: HEK293T cells

Fig. 4: SSL10 induces necroptosis by direct interacting with the extracellular domain of TNFR1 (TNFR1ECD).a Wild type (WT) or Tnfrsf1a knockout (KO) HEK293T cells were treated with SSL10 at different concentrations for 30 min, and the SSL proteins bound to the cell surface were detected by flow cytometry after FITC-conjugated anti-His-tag antibody staining. b WT or Tnfrsf1a KO cells were treated with 2 μM SSL10 for 48 h at 37 °C and 5% CO2, and the LDH release was detected. c WT or Tnfrsf1a KO HEK293T cells were challenged with 2 μM SSL10 for 48 h and the cell viability was measured by flow cytometry after Annexin V/PI staining. The dot plot (left) is representative of three independent experiments, and the results of quantification (right) are shown as a bar graph. d Depolarization of the mitochondrial membrane of WT or Tnfrsf1a KO HEK293T cells treated with SSL10 was measured by flow cytometry after JC-1 staining. The dot plot (left) is representative of three independent experiments, and the results of quantification (right) are shown as a bar graph. e Immunoblotting of SSL10 after pull-down with MBP-TNFR1ECD. f ITC assays for SSL10 binding to MBP-TNFR1ECD or MBP control protein. Cells treated with 2 μM SSL7 were used as a negative protein control throughout the experiments. All data are presented as the means ± SD from three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001 compared to the Ctrl cells (buffer-treated cells). #p < 0.05; ##p < 0.01; ###p < 0.001 compared to the SSL10 treated group, by two-way ANOVA.

3). Guo J et al. Pterostilbene protects the optic nerves and retina in a murine model of experimental autoimmune encephalomyelitis via activation of SIRT1 signaling. NEUROSCIENCE 2022 Apr 1;487:35-46. (PubMed: 35090883) [IF=3.3]

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