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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Mouse
Application:
WB 1:1000-1:5000, IF/ICC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
All
Clonality:
Monoclonal [T235]
Specificity:
The mCherry tag antibody can recognize mCherry and mCherry-tag fusion proteins.
RRID:
AB_2843441
Cite Format: Affinity Biosciences Cat# T0090, RRID:AB_2843441.
Conjugate:
Unconjugated.
Purification:
affinity purification.
Storage:
Store at -20°C. Stable for one year from the date of shipment.1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

mCherry,mCherry antibody

References

1). YY1 Oligomerization Is Regulated by Its OPB Domain and Competes with Its Regulation of Oncoproteins. Cancers, 2022 (PubMed: 35406384) [IF=5.2]

Application: WB    Species: Human    Sample:

Figure 2. Identification of the binding sites responsible for YY1 dimerization by co-immunoprecipitation (co-IP). (A) Diagrams of HA- and Flag-tagged YY1 wt and its mutant proteins used for co-IP studies. (B,C) Co-IP studies to determine the binding sites responsible for YY1 dimerization. HA-YY1 wt (B) or its ΔZF mutant (C) expression plasmid was individually cotransfected with Flag-EGFP-YY1 wt and mutant expression vectors, as well as an empty vector (EV). Cell lysates were co-IPed by a Flag antibody, and HA and Flag antibodies were used in Western blot analyses. (D) Schematic diagram of a predicted YY1 dimerization mode. (E) Effects of the OPB on intermolecular interaction of YY1. Different amounts of mCherry-cont, mCherry-OPB and mCherry plasmids were individually cotransfected with Flag-EGFP-YY1(ΔZF) and HA-YY1(ΔZF) expression vectors, followed by co-IP of the Flag antibody. HA, mCherry and Flag antibodies were used in Western blot analyses.

2). RPG interacts with E3-ligase CERBERUS to mediate rhizobial infection in Lotus japonicus. PLOS Genetics, 2023 (PubMed: 36735729) [IF=4.5]

3). CRMP2 modulates mossy fiber sprouting in dentate gyrus of pilocarpine induced rat model of epilepsy. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2022 (PubMed: 35334412) [IF=3.1]

4). Transplanting Rac1-silenced bone marrow mesenchymal stem cells promote neurological function recovery in TBI mice. Aging (Albany NY), 2021 (PubMed: 33411679) [IF=0.4]

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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