mCherry-Tag Antibody - #T0090
(4)
(2)
| Product: | mCherry-Tag Antibody |
| Catalog: | T0090 |
| Description: | Mouse monoclonal antibody to mCherry-Tag |
| Application: | WB IF/ICC ELISA |
| Reactivity: | All |
| Uniprot: | |
| RRID: | AB_2843441 |
Product Info
Source:
Mouse
Application:
WB 1:1000-1:5000, IF/ICC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
All
Clonality:
Monoclonal [T235]
Specificity:
The mCherry tag antibody can recognize mCherry and mCherry-tag fusion proteins.
RRID:
AB_2843441
Cite Format: Affinity Biosciences Cat# T0090, RRID:AB_2843441.
Cite Format: Affinity Biosciences Cat# T0090, RRID:AB_2843441.
Conjugate:
Unconjugated.
Purification:
affinity purification.
Storage:
Store at -20°C. Stable for one year from the date of shipment.1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
mCherry,mCherry antibody
References
1). YY1 Oligomerization Is Regulated by Its OPB Domain and Competes with Its Regulation of Oncoproteins. Cancers, 2022
(PubMed: 35406384)
[IF=5.2]
Application: WB Species: Human Sample:
Figure 2. Identification of the binding sites responsible for YY1 dimerization by co-immunoprecipitation (co-IP). (A) Diagrams of HA- and Flag-tagged YY1 wt and its mutant proteins used for co-IP studies. (B,C) Co-IP studies to determine the binding sites responsible for YY1 dimerization. HA-YY1 wt (B) or its ΔZF mutant (C) expression plasmid was individually cotransfected with Flag-EGFP-YY1 wt and mutant expression vectors, as well as an empty vector (EV). Cell lysates were co-IPed by a Flag antibody, and HA and Flag antibodies were used in Western blot analyses. (D) Schematic diagram of a predicted YY1 dimerization mode. (E) Effects of the OPB on intermolecular interaction of YY1. Different amounts of mCherry-cont, mCherry-OPB and mCherry plasmids were individually cotransfected with Flag-EGFP-YY1(ΔZF) and HA-YY1(ΔZF) expression vectors, followed by co-IP of the Flag antibody. HA, mCherry and Flag antibodies were used in Western blot analyses.
2). RPG interacts with E3-ligase CERBERUS to mediate rhizobial infection in Lotus japonicus. PLOS Genetics, 2023
(PubMed: 36735729)
[IF=4.0]
3). CRMP2 modulates mossy fiber sprouting in dentate gyrus of pilocarpine induced rat model of epilepsy. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2022
(PubMed: 35334412)
[IF=2.5]
4). Transplanting Rac1-silenced bone marrow mesenchymal stem cells promote neurological function recovery in TBI mice. Aging (Albany NY), 2021
(PubMed: 33411679)
[IF=0.4]
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