Phospho-Gab2 (Tyr452) Antibody | Affinity Biosciences

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Product:	Phospho-Gab2 (Tyr452) Antibody
Catalog:	AF0059
Description:	Rabbit polyclonal antibody to Phospho-Gab2 (Tyr452)
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	75kDa; 74kD(Calculated).
Uniprot:	Q9UQC2
RRID:	AB_2834102

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%), Xenopus(86%)

Clonality:

Polyclonal

Specificity:

Phospho-Gab2 (Tyr452) Antibody detects endogenous levels of Gab2 only when phosphorylated at Tyrosine 452.

RRID:

AB_2834102
Cite Format: Affinity Biosciences Cat# AF0059, RRID:AB_2834102.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

GAB 2; Gab2; GAB2_HUMAN; Grb 2 associated binder 2; GRB 2 associated binding protein 2; Grb2 associated binder 2; GRB2 associated binder 2 pp100; GRB2 associated binding protein 2; GRB2-associated binder 2; GRB2-associated-binding protein 2; Growth factor receptor bound protein 2 associated protein 2; Growth factor receptor bound protein 2-associated protein 2; KIAA0571; p97; PH domain containing adaptor molecule p97; pp100;

Immunogens

Immunogen:

Uniprot:

Q9UQC2(GAB2_HUMAN) >>Visit HPA database.

Gene(ID):

GAB2(9846)

Description:

This gene is a member of the GRB2-associated binding protein (GAB) gene family and is similar to the GAB1 gene. These proteins contain pleckstrin homology (PH) domain, and bind SHP2 tyrosine phosphatase and GRB2 adapter protein. They act as adapters for transmitting various signals in response to stimuli through cytokine and growth factor receptors, and T- and B-cell antigen receptors. The protein encoded by this gene is the principal activator of phosphatidylinositol-3 kinase in response to activation of the high affinity IgE receptor. Two alternatively spliced transcripts encoding different isoforms have been described for this gene.

Sequence:

Q9UQC2 GAB2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSGGGDVVCTGWLRKSPPEKKLRRYAWKKRWFILRSGRMSGDPDVLEYYKNDHSKKPLRIINLNFCEQVDAGLTFNKKELQDSFVFDIKTSERTFYLVAETEEDMNKWVQSICQICGFNQAEESTDSLRNVSSAGHGPRSSPAELSSSSQHLLRERKSSAPSHSSQPTLFTFEPPVSNHMQPTLSTSAPQEYLYLHQCISRRAENARSASFSQGTRASFLMRSDTAVQKLAQGNGHCVNGISGQVHGFYSLPKPSRHNTEFRDSTYDLPRSLASHGHTKGSLTGSETDNEDVYTFKTPSNTLCREFGDLLVDNMDVPATPLSAYQIPRTFTLDKNHNAMTVATPGDSAIAPPPRPPKPSQAETPRWGSPQQRPPISENSRSVAATIPRRNTLPAMDNSRLHRASSCETYEYPQRGGESAGRSAESMSDGVGSFLPGKMIVGRSDSTNSEDNYVPMNPGSSTLLAMERAGDNSQSVYIPMSPGAHHFDSLGYPSTTLPVHRGPSRGSEIQPPPVNRNLKPDRKAKPTPLDLRNNTVIDELPFKSPITKSWSRANHTFNSSSSQYCRPISTQSITSTDSGDSEENYVPMQNPVSASPVPSGTNSPAPKKSTGSVDYLALDFQPSSPSPHRKPSTSSVTSDEKVDYVQVDKEKTQALQNTMQEWTDVRQSSEPSKGAKL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UQC2 As Substrate

Q9UQC2

Site	PTM Type	Enzyme	Source
	Phosphorylation		Uniprot
S2	Phosphorylation		Uniprot
Y48	Phosphorylation		Uniprot
Y49	Phosphorylation		Uniprot
K56	Acetylation		Uniprot
S133	Phosphorylation		Uniprot
S140	Phosphorylation		Uniprot
S141	Phosphorylation		Uniprot
S146	Phosphorylation		Uniprot
S147	Phosphorylation		Uniprot
S148	Phosphorylation		Uniprot
S149	Phosphorylation		Uniprot
S159	Phosphorylation	P31749 (AKT1)	Uniprot
S164	Phosphorylation		Uniprot
T171	Phosphorylation		Uniprot
S177	Phosphorylation		Uniprot
Y194	Phosphorylation		Uniprot
S208	Phosphorylation		Uniprot
S210	Phosphorylation		Uniprot
S218	Phosphorylation		Uniprot
S223	Phosphorylation		Uniprot
Y249	Phosphorylation		Uniprot
S250	Phosphorylation		Uniprot
S264	Phosphorylation		Uniprot
T265	Phosphorylation		Uniprot
Y266	Phosphorylation		Uniprot
T278	Phosphorylation		Uniprot
S281	Phosphorylation		Uniprot
S285	Phosphorylation		Uniprot
T287	Phosphorylation		Uniprot
Y293	Phosphorylation		Uniprot
T294	Phosphorylation		Uniprot
T319	Phosphorylation		Uniprot
S322	Phosphorylation		Uniprot
Y324	Phosphorylation		Uniprot
T331	Phosphorylation		Uniprot
S368	Phosphorylation		Uniprot
T385	Phosphorylation		Uniprot
T391	Phosphorylation		Uniprot
S404	Phosphorylation		Uniprot
S405	Phosphorylation		Uniprot
T408	Phosphorylation		Uniprot
Y409	Phosphorylation		Uniprot
Y411	Phosphorylation		Uniprot
S422	Phosphorylation		Uniprot
S425	Phosphorylation		Uniprot
S427	Phosphorylation		Uniprot
S448	Phosphorylation		Uniprot
Y452	Phosphorylation	P08631 (HCK)	Uniprot
S459	Phosphorylation		Uniprot
S472	Phosphorylation		Uniprot
S474	Phosphorylation		Uniprot
Y476	Phosphorylation		Uniprot
S480	Phosphorylation		Uniprot
S488	Phosphorylation		Uniprot
Y491	Phosphorylation		Uniprot
K542	Methylation		Uniprot
S543	Phosphorylation		Uniprot
T546	Phosphorylation		Uniprot
S550	Phosphorylation		Uniprot
Y563	Phosphorylation		Uniprot
Y584	Phosphorylation		Uniprot
S611	Phosphorylation		Uniprot
Y614	Phosphorylation	P43403 (ZAP70)	Uniprot
S622	Phosphorylation		Uniprot
S623	Phosphorylation	P28482 (MAPK1) , P27361 (MAPK3)	Uniprot
S625	Phosphorylation		Uniprot
S631	Phosphorylation		Uniprot
T632	Phosphorylation		Uniprot
S633	Phosphorylation		Uniprot
S634	Phosphorylation		Uniprot
T636	Phosphorylation		Uniprot
S637	Phosphorylation		Uniprot
Y643	Phosphorylation	O60674 (JAK2)	Uniprot
T651	Phosphorylation		Uniprot
T657	Phosphorylation		Uniprot

Research Backgrounds

Q9UQC2_GAB2_HUMAN

Function:

Adapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis.

PTMs:

Phosphorylated on tyrosine residue(s) by the thrombopoietin receptor (TPOR), stem cell factor receptor (SCFR), and T-cell and B-cell antigen receptors, gp130, IL-2R and IL-3R (By similarity). Phosphorylated upon stimulation of TNFRSF11A/RANK by TNFSF11/RANKL (By similarity). Phosphorylated upon EGF stimulation. Phosphorylated on tyrosine residues by HCK upon IL6 signaling.

Dephosphorylated by PTPN11.

Subcellular Location:

Cytoplasm. Cell membrane.

Subunit Structure:

Interacts with SHC1; may mediate interaction with receptors (By similarity). Interacts with SYK (By similarity). Interacts with PI-3 kinase. Interacts with GRB2 (via SH3 2 domain). Interacts (phosphorylated) with PTPN11. Interacts with TNFRSF11A (via cytoplasmic domain). Interacts (phosphorylated) with 14-3-3 family proteins SFN, YWHAB, YWHAE, YWHAG, YWHAH, YWHAQ and YWHAZ; prevents interaction with GRB2 and attenuates GAB2 signaling. Interacts with HCK.

Family&Domains:

The SH3-binding motifs mediate interaction with SHC1 and GRB2.

The PH domain mediates phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,4-bisphosphate binding.

Belongs to the GAB family.

Research Fields

· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia. (View pathway)

· Organismal Systems > Development > Osteoclast differentiation. (View pathway)

· Organismal Systems > Immune system > Fc epsilon RI signaling pathway. (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)

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Phospho-Gab2 (Tyr452) Antibody - #AF0059