PLD2 Antibody | Affinity Biosciences

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Western blot analysis of PLD2 using HuvEc whole cell lysates.

Product:	PLD2 Antibody
Catalog:	DF9752
Description:	Rabbit polyclonal antibody to PLD2
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Horse, Sheep
Mol.Wt.:	106 kDa; 106kD(Calculated).
Uniprot:	O14939
RRID:	AB_2842947

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(100%), Horse(100%), Sheep(100%)

Clonality:

Polyclonal

Specificity:

PLD2 Antibody detects endogenous levels of total PLD2.

RRID:

AB_2842947
Cite Format: Affinity Biosciences Cat# DF9752, RRID:AB_2842947.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Choline phosphatase 2; EC 3.1.4.4; hPLD 2; hPLD2; mPLD2; Phosphatidylcholine hydrolyzing phospholipase D2; Phosphatidylcholine-hydrolyzing phospholipase D2; Phospholipase D2; PhospholipaseD2; PLD 2; PLD1C; Pld2; PLD2_HUMAN; Pldc; rPLD2;

Immunogens

Immunogen:

Uniprot:

O14939(PLD2_HUMAN) >>Visit HPA database.

Gene(ID):

PLD2(5338)

Expression:

O14939 PLD2_HUMAN:

Ubiquitous.

Sequence:

O14939 PLD2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTATPESLFPTGDELDSSQLQMESDEVDTLKEGEDPADRMHPFLAIYELQSLKVHPLVFAPGVPVTAQVVGTERYTSGSKVGTCTLYSVRLTHGDFSWTTKKKYRHFQELHRDLLRHKVLMSLLPLARFAVAYSPARDAGNREMPSLPRAGPEGSTRHAASKQKYLENYLNRLLTMSFYRNYHAMTEFLEVSQLSFIPDLGRKGLEGMIRKRSGGHRVPGLTCCGRDQVCYRWSKRWLVVKDSFLLYMCLETGAISFVQLFDPGFEVQVGKRSTEARHGVRIDTSHRSLILKCSSYRQARWWAQEITELAQGPGRDFLQLHRHDSYAPPRPGTLARWFVNGAGYFAAVADAILRAQEEIFITDWWLSPEVYLKRPAHSDDWRLDIMLKRKAEEGVRVSILLFKEVELALGINSGYSKRALMLLHPNIKVMRHPDQVTLWAHHEKLLVVDQVVAFLGGLDLAYGRWDDLHYRLTDLGDSSESAASQPPTPRPDSPATPDLSHNQFFWLGKDYSNLITKDWVQLDRPFEDFIDRETTPRMPWRDVGVVVHGLPARDLARHFIQRWNFTKTTKAKYKTPTYPYLLPKSTSTANQLPFTLPGGQCTTVQVLRSVDRWSAGTLENSILNAYLHTIRESQHFLYIENQFFISCSDGRTVLNKVGDEIVDRILKAHKQGWCYRVYVLLPLLPGFEGDISTGGGNSIQAILHFTYRTLCRGEYSILHRLKAAMGTAWRDYISICGLRTHGELGGHPVSELIYIHSKVLIADDRTVIIGSANINDRSLLGKRDSELAVLIEDTETEPSLMNGAEYQAGRFALSLRKHCFGVILGANTRPDLDLRDPICDDFFQLWQDMAESNANIYEQIFRCLPSNATRSLRTLREYVAVEPLATVSPPLARSELTQVQGHLVHFPLKFLEDESLLPPLGSKEGMIPLEVWT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Pig

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O14939 As Substrate

O14939

Site	PTM Type	Enzyme	Source
T29	Phosphorylation		Uniprot
T72	Phosphorylation		Uniprot
S77	Phosphorylation		Uniprot
S79	Phosphorylation		Uniprot
K80	Ubiquitination		Uniprot
T99	Phosphorylation		Uniprot
T100	Phosphorylation		Uniprot
K118	Ubiquitination		Uniprot
S134	Phosphorylation	Q00535 (CDK5)	Uniprot
S146	Phosphorylation		Uniprot
T156	Phosphorylation		Uniprot
S161	Phosphorylation		Uniprot
Y169	Phosphorylation		Uniprot
T175	Phosphorylation		Uniprot
Y179	Phosphorylation	P00533 (EGFR)	Uniprot
S213	Phosphorylation		Uniprot
S243	Phosphorylation	P17252 (PRKCA)	Uniprot
T252	Phosphorylation	P17252 (PRKCA)	Uniprot
K292	Methylation		Uniprot
Y296	Phosphorylation	P00533 (EGFR)	Uniprot
K388	Methylation		Uniprot
K388	Ubiquitination		Uniprot
Y415	Phosphorylation	P52333 (JAK3)	Uniprot
S416	Phosphorylation		Uniprot
T488	Phosphorylation		Uniprot
Y511	Phosphorylation	P12931 (SRC)	Uniprot
T566	Phosphorylation	Q05655 (PRKCD)	Uniprot
Y573	Phosphorylation		Uniprot
Y578	Phosphorylation		Uniprot
Y580	Phosphorylation		Uniprot
K656	Ubiquitination		Uniprot

Research Backgrounds

O14939_PLD2_HUMAN

Function:

May have a role in signal-induced cytoskeletal regulation and/or endocytosis.

PTMs:

Phosphorylated by FGR.

Subcellular Location:

Membrane>Peripheral membrane protein.

Tissue Specificity:

Ubiquitous.

Subunit Structure:

Interacts with EGFR (By similarity). Interacts with PIP5K1B.

Family&Domains:

Belongs to the phospholipase D family.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)

· Human Diseases > Cancers: Overview > Choline metabolism in cancer. (View pathway)

· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.

· Metabolism > Lipid metabolism > Ether lipid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)

· Organismal Systems > Nervous system > Glutamatergic synapse.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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PLD2 Antibody - #DF9752