RFP-Tag Antibody - #T0055
(3)
(3)
| Product: | RFP-Tag Antibody |
| Catalog: | T0055 |
| Source: | Mouse |
| Application: | WB |
| Reactivity: | All |
| Uniprot: | |
| RRID: | AB_2846170 |
Product Info
Source:
Mouse
Application:
WB(1:1000-1:10000)
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
All
Clonality:
Monoclonal [F897]
RRID:
AB_2846170
Cite Format: Affinity Biosciences Cat# T0055, RRID:AB_2846170.
Cite Format: Affinity Biosciences Cat# T0055, RRID:AB_2846170.
Conjugate:
Unconjugated.
Purification:
Protein G purification.
Storage:
Mouse IgG1 in 10 mM PBS (pH 7.4), 0.1mg/ml BSA , 50% glycerol and 1% ProClin™ 300. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
DsRed;GFP like chromoprotein;red fluorescent protein;RFP;
References
1). Wang B et al. The positive regulation loop between NRF1 and NONO-TFE3 fusion promotes phase separation and aggregation of NONO-TFE3 in NONO-TFE3 tRCC. Int J Biol Macromol 2021 Feb 13;176:437-447. (PubMed: 33592266)
[IF=8.025]
Application: WB Species: human Sample: HEK293T cells
Fig. 3.| The intrinsically disordered regions of NONO fragment induced accumulation of NONO-TFE3.. (I) HEK293T cells were transiently transfected vector-RFP or vector expressing IDR1 and IDR2 and the protein levels of RFP were examined by Western blot at 24 h and 48 h.
2). Zhao J et al. Study of the effects of 3.1 THz radiation on the expression of recombinant red fluorescent protein (RFP) in E. coli. Biomed Opt Express 2020 Jun 22;11(7):3890-3899. (PubMed: 33014573)
[IF=3.562]
3). Chen Y et al. A drug screening toolkit based on the–1 ribosomal frameshifting of SARS-CoV-2. Heliyon 2020 Aug;6(8):e04793. (PubMed: 32869005)
Application: IF/ICC Species: Human Sample: 16HBE cells
Figure 4
Drugs that increased the –1 PRF frequencies. (a–c) Functions of the the inhibitory drugs were detected by flow cytometry (mean ± SD; n = 5 tests; ∗P < 0.05; ∗∗P < 0.01; by two-tailed Student t test). (d) Microscopic image of fluorescent protein expression (scale bar = 200 um). (e) Western blot analysis of GFP and GFP–RFP fusion protein. (f) Western blot analysis of RFP and GFP–RFP fusion protein. (g) Densitometric quantification of GFP–RFP fusion protein levels in western blot normalized against GFP in 16HBE cells (mean ± SD; n = 3 tests; ∗P < 0.05 by the two-tailed Student t test). Abbreviations: Ery, Erythromycin; Hup, (–)-huperzine A; NC, negative control; PC, positive control; Tri, trimethobenzamide.
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