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*The optimal dilutions should be determined by the end user.
Monoclonal [F897]
Cite Format: Affinity Biosciences Cat# T0055, RRID:AB_2846170.
Protein G purification.
Mouse IgG1 in 10 mM PBS (pH 7.4), 0.1mg/ml BSA , 50% glycerol and 1% ProClin™ 300. Store at -20 °C. Stable for 12 months from date of receipt.


DsRed;GFP like chromoprotein;red fluorescent protein;RFP;


1). Wang B et al. The positive regulation loop between NRF1 and NONO-TFE3 fusion promotes phase separation and aggregation of NONO-TFE3 in NONO-TFE3 tRCC. Int J Biol Macromol 2021 Feb 13;176:437-447. (PubMed: 33592266) [IF=8.025]

Application: WB    Species: human    Sample: HEK293T cells

Fig. 3.| The intrinsically disordered regions of NONO fragment induced accumulation of NONO-TFE3.. (I) HEK293T cells were transiently transfected vector-RFP or vector expressing IDR1 and IDR2 and the protein levels of RFP were examined by Western blot at 24 h and 48 h.

2). Zhao J et al. Study of the effects of 3.1 THz radiation on the expression of recombinant red fluorescent protein (RFP) in E. coli. Biomed Opt Express 2020 Jun 22;11(7):3890-3899. (PubMed: 33014573) [IF=3.562]

3). Chen Y et al. A drug screening toolkit based on the–1 ribosomal frameshifting of SARS-CoV-2. Heliyon 2020 Aug;6(8):e04793. (PubMed: 32869005)

Application: IF/ICC    Species: Human    Sample: 16HBE cells

Figure 4 Drugs that increased the –1 PRF frequencies. (a–c) Functions of the the inhibitory drugs were detected by flow cytometry (mean ± SD; n = 5 tests; ∗P < 0.05; ∗∗P < 0.01; by two-tailed Student t test). (d) Microscopic image of fluorescent protein expression (scale bar = 200 um). (e) Western blot analysis of GFP and GFP–RFP fusion protein. (f) Western blot analysis of RFP and GFP–RFP fusion protein. (g) Densitometric quantification of GFP–RFP fusion protein levels in western blot normalized against GFP in 16HBE cells (mean ± SD; n = 3 tests; ∗P < 0.05 by the two-tailed Student t test). Abbreviations: Ery, Erythromycin; Hup, (–)-huperzine A; NC, negative control; PC, positive control; Tri, trimethobenzamide.

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