DMPK Antibody - #AF0579
| Product: | DMPK Antibody |
| Catalog: | AF0579 |
| Description: | Rabbit polyclonal antibody to DMPK |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human |
| Mol.Wt.: | 70kDa; 69kD(Calculated). |
| Uniprot: | Q09013 |
| RRID: | AB_2834246 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF0579, RRID:AB_2834246.
Fold/Unfold
DM 1; DM; DM kinase; DM protein kinase; DM-kinase; DM1; DM1 protein kinase; DM1PK; DMK; DMPK; DMPK_HUMAN; Dystrophia myotonica 1; Dystrophia myotonica protein kinase; MDPK; MT PK; MT-PK; Myotonic dystrophy associated protein kinase; Myotonic dystrophy protein kinase; Myotonin protein kinase A; Myotonin protein kinase; Myotonin-protein kinase; Thymopoietin homolog;
Immunogens
A synthesized peptide derived from human DMPK, corresponding to a region within N-terminal amino acids.
Most isoforms are expressed in many tissues including heart, skeletal muscle, liver and brain, except for isoform 2 which is only found in the heart and skeletal muscle, and isoform 14 which is only found in the brain, with high levels in the striatum, cerebellar cortex and pons.
- Q09013 DMPK_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSAEVRLRRLQQLVLDPGFLGLEPLLDLLLGVHQELGASELAQDKYVADFLQWAEPIVVRLKEVRLQRDDFEILKVIGRGAFSEVAVVKMKQTGQVYAMKIMNKWDMLKRGEVSCFREERDVLVNGDRRWITQLHFAFQDENYLYLVMEYYVGGDLLTLLSKFGERIPAEMARFYLAEIVMAIDSVHRLGYVHRDIKPDNILLDRCGHIRLADFGSCLKLRADGTVRSLVAVGTPDYLSPEILQAVGGGPGTGSYGPECDWWALGVFAYEMFYGQTPFYADSTAETYGKIVHYKEHLSLPLVDEGVPEEARDFIQRLLCPPETRLGRGGAGDFRTHPFFFGLDWDGLRDSVPPFTPDFEGATDTCNFDLVEDGLTAMVSGGGETLSDIREGAPLGVHLPFVGYSYSCMALRDSEVPGPTPMELEAEQLLEPHVQAPSLEPSVSPQDETAEVAVPAAVPAAEAEAEVTLRELQEALEEEVLTRQSLSREMEAIRTDNQNFASQLREAEARNRDLEAHVRQLQERMELLQAEGATAVTGVPSPRATDPPSHLDGPPAVAVGQCPLVGPGPMHRRHLLLPARVPRPGLSEALSLLLFAVVLSRAAALGCIGLVAHAGQLTAVWRRPGAARAP
PTMs - Q09013 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | Uniprot | |
| K75 | Ubiquitination | Uniprot | |
| K197 | Ubiquitination | Uniprot | |
| T533 | Phosphorylation | Uniprot | |
| T536 | Phosphorylation | Uniprot | |
| S540 | Phosphorylation | Uniprot | |
| R579 | Methylation | Uniprot |
PTMs - Q09013 As Enzyme
Research Backgrounds
Non-receptor serine/threonine protein kinase which is necessary for the maintenance of skeletal muscle structure and function. May play a role in myocyte differentiation and survival by regulating the integrity of the nuclear envelope and the expression of muscle-specific genes. May also phosphorylate PPP1R12A and inhibit the myosin phosphatase activity to regulate myosin phosphorylation. Also critical to the modulation of cardiac contractility and to the maintenance of proper cardiac conduction activity probably through the regulation of cellular calcium homeostasis. Phosphorylates PLN, a regulator of calcium pumps and may regulate sarcoplasmic reticulum calcium uptake in myocytes. May also phosphorylate FXYD1/PLM which is able to induce chloride currents. May also play a role in synaptic plasticity.
Phosphorylated. Autophosphorylates. Phosphorylation by RAF1 may result in activation of DMPK.
Proteolytic processing of the C-terminus may remove the transmembrane domain and release the kinase from membranes stimulating its activity.
Endoplasmic reticulum membrane>Single-pass type IV membrane protein>Cytoplasmic side. Nucleus outer membrane>Single-pass type IV membrane protein>Cytoplasmic side. Mitochondrion outer membrane>Single-pass type IV membrane protein. Sarcoplasmic reticulum membrane. Cell membrane. Cytoplasm>Cytosol.
Note: Localizes to sarcoplasmic reticulum membranes of cardiomyocytes.
Mitochondrion membrane.
Mitochondrion membrane.
Most isoforms are expressed in many tissues including heart, skeletal muscle, liver and brain, except for isoform 2 which is only found in the heart and skeletal muscle, and isoform 14 which is only found in the brain, with high levels in the striatum, cerebellar cortex and pons.
Homodimer; homodimerization stimulates the kinase activity. Interacts with HSPB2; may enhance DMPK kinase activity. Interacts with PLN; phosphorylates PLN. May interact with RAC1; may regulate DMPK kinase activity. Interacts with LMNA; may regulate nuclear envelope stability.
The coiled coil domain is required for homodimerization and regulates the enzymatic activity.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.
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