RBP5 Antibody - #AF0720

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Product: RBP5 Antibody
Catalog: AF0720
Description: Rabbit polyclonal antibody to RBP5
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 15kDa; 16kD(Calculated).
Uniprot: P82980
RRID: AB_2834242

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 100ul $280 In stock
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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Zebrafish(88%), Bovine(86%), Horse(100%), Sheep(88%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
RBP5 Antibody detects endogenous levels of total RBP5.
RRID:
AB_2834242
Cite Format: Affinity Biosciences Cat# AF0720, RRID:AB_2834242.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cellular retinol-binding protein III; CRBP III; CRBP-III; CRBP3; CRBPIII; HRBPiso; OTTHUMP00000238163; Putative cellular retinol binding protein CRBP III; RBP5; RET5_HUMAN; Retinol binding protein; Retinol binding protein 5; Retinol binding protein 5, cellular; Retinol-binding protein 5;

Immunogens

Immunogen:
Uniprot:

P82980(RET5_HUMAN) >>Visit HPA database.

Gene(ID):
RBP5(83758)
Expression:
P82980 RET5_HUMAN:

Higher expression in adult kidney and liver and to a lesser extent in adult and fetal spleen, adult lymph nodes and appendix, and fetal liver and kidney. Strongly decreased in hepatocellular carcinoma tissues (at protein level).

Description:
RBP5 Intracellular transport of retinol. Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. Note: This description may include information from UniProtKB.
Sequence:
MPPNLTGYYRFVSQKNMEDYLQALNISLAVRKIALLLKPDKEIEHQGNHMTVRTLSTFRNYTVQFDVGVEFEEDLRSVDGRKCQTIVTWEEEHLVCVQKGEVPNRGWRHWLEGEMLYLELTARDAVCEQVFRKVR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Chicken
100
Rabbit
100
Sheep
88
Zebrafish
88
Bovine
86
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Intracellular transport of retinol.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Higher expression in adult kidney and liver and to a lesser extent in adult and fetal spleen, adult lymph nodes and appendix, and fetal liver and kidney. Strongly decreased in hepatocellular carcinoma tissues (at protein level).

Family&Domains:

Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.

Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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