Product: hnRNP M Antibody
Catalog: AF0481
Description: Rabbit polyclonal antibody to hnRNP M
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 85,78kDa; 78kD(Calculated).
Uniprot: P52272
RRID: AB_2834240

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
hnRNP M Antibody detects endogenous levels of total hnRNP M.
RRID:
AB_2834240
Cite Format: Affinity Biosciences Cat# AF0481, RRID:AB_2834240.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CEA receptor; CEAR; Heterogeneous nuclear ribonucleoprotein M; Heterogenous nuclear ribonucleoprotein M4; hnRNA binding protein M4; hnRNP M; Hnrnpm; HNRNPM4; HNRPM; HNRPM_HUMAN; HNRPM4; HTGR1; M3; M4; M4 protein; N-acetylglucosamine receptor 1; NAGR1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Description:
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs. This protein also constitutes a monomer of the N-acetylglucosamine-specific receptor which is postulated to trigger selective recycling of immature GlcNAc-bearing thyroglobulin molecules. Multiple alternatively spliced transcript variants are known for this gene but only two transcripts has been isolated.
Sequence:
MAAGVEAAAEVAATEIKMEEESGAPGVPSGNGAPGPKGEGERPAQNEKRKEKNIKRGGNRFEPYANPTKRYRAFITNIPFDVKWQSLKDLVKEKVGEVTYVELLMDAEGKSRGCAVVEFKMEESMKKAAEVLNKHSLSGRPLKVKEDPDGEHARRAMQKVMATTGGMGMGPGGPGMITIPPSILNNPNIPNEIIHALQAGRLGSTVFVANLDYKVGWKKLKEVFSMAGVVVRADILEDKDGKSRGIGTVTFEQSIEAVQAISMFNGQLLFDRPMHVKMDERALPKGDFFPPERPQQLPHGLGGIGMGLGPGGQPIDANHLNKGIGMGNIGPAGMGMEGIGFGINKMGGMEGPFGGGMENMGRFGSGMNMGRINEILSNALKRGEIIAKQGGGGGGGSVPGIERMGPGIDRLGGAGMERMGAGLGHGMDRVGSEIERMGLVMDRMGSVERMGSGIERMGPLGLDHMASSIERMGQTMERIGSGVERMGAGMGFGLERMAAPIDRVGQTIERMGSGVERMGPAIERMGLSMERMVPAGMGAGLERMGPVMDRMATGLERMGANNLERMGLERMGANSLERMGLERMGANSLERMGPAMGPALGAGIERMGLAMGGGGGASFDRAIEMERGNFGGSFAGSFGGAGGHAPGVARKACQIFVRNLPFDFTWKMLKDKFNECGHVLYADIKMENGKSKGCGVVKFESPEVAERACRMMNGMKLSGREIDVRIDRNA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
75
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P52272 As Substrate

Site PTM Type Enzyme
A2 Acetylation
T14 Phosphorylation
K17 Sumoylation
S22 Phosphorylation
S29 Phosphorylation
K37 Sumoylation
K37 Ubiquitination
K48 Acetylation
K48 Ubiquitination
K52 Methylation
K55 Sumoylation
R56 Methylation
Y64 Phosphorylation
T68 Phosphorylation
K69 Acetylation
K69 Ubiquitination
T76 Phosphorylation
K83 Acetylation
K83 Sumoylation
K83 Ubiquitination
S86 Phosphorylation
K88 Sumoylation
K88 Ubiquitination
K92 Ubiquitination
K94 Ubiquitination
T99 Phosphorylation
Y100 Phosphorylation
K110 Acetylation
K110 Sumoylation
K110 Ubiquitination
C114 S-Nitrosylation
K120 Sumoylation
K120 Ubiquitination
K126 Sumoylation
K126 Ubiquitination
K127 Sumoylation
K127 Ubiquitination
K134 Acetylation
K134 Sumoylation
K134 Ubiquitination
S136 Phosphorylation
S138 Phosphorylation
K143 Sumoylation
K143 Ubiquitination
K145 Sumoylation
K145 Ubiquitination
S204 Phosphorylation
T205 Phosphorylation
Y213 Phosphorylation
K214 Acetylation
K214 Ubiquitination
K218 Ubiquitination
K221 Sumoylation
K221 Ubiquitination
K239 Acetylation
K239 Sumoylation
K239 Ubiquitination
K242 Sumoylation
K242 Ubiquitination
K277 Acetylation
K277 Sumoylation
K277 Ubiquitination
K285 Ubiquitination
K322 Sumoylation
K322 Ubiquitination
K345 Ubiquitination
S365 Phosphorylation
R371 Methylation
S377 Phosphorylation
K381 Acetylation
K381 Sumoylation
K381 Ubiquitination
K388 Sumoylation
K388 Ubiquitination
S397 Phosphorylation
R410 Methylation
R429 Methylation
S432 Phosphorylation O14757 (CHEK1)
R443 Methylation
S446 Phosphorylation
S452 Phosphorylation
S467 Phosphorylation
S468 Phosphorylation
T475 Phosphorylation
S481 Phosphorylation
R485 Methylation
R496 Methylation
R503 Methylation
T507 Phosphorylation
S513 Phosphorylation
S528 Phosphorylation
R543 Methylation
R550 Methylation
R565 Methylation
S575 Phosphorylation
S588 Phosphorylation
S618 Phosphorylation
R621 Methylation
R627 Methylation
S633 Phosphorylation
S637 Phosphorylation
K651 Sumoylation
K651 Ubiquitination
T665 Phosphorylation
K667 Acetylation
K667 Sumoylation
K667 Ubiquitination
K670 Ubiquitination
K672 Acetylation
K672 Sumoylation
K672 Ubiquitination
C676 S-Nitrosylation
Y681 Phosphorylation
K685 Acetylation
K685 Sumoylation
K685 Ubiquitination
K690 Ubiquitination
K692 Ubiquitination
C694 S-Nitrosylation
K698 Acetylation
K698 Sumoylation
K698 Ubiquitination
S701 Phosphorylation
C709 S-Nitrosylation
K716 Sumoylation
K716 Ubiquitination

Research Backgrounds

Function:

Pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines.

PTMs:

Sumoylated.

Subcellular Location:

Nucleus>Nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Identified in the spliceosome C complex.

Research Fields

· Genetic Information Processing > Transcription > Spliceosome.

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