HNF4 alpha /gamma Antibody - #AF0534
| Product: | HNF4 alpha /gamma Antibody |
| Catalog: | AF0534 |
| Description: | Rabbit polyclonal antibody to HNF4 alpha /gamma |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 52kDa; 53kD,46kD(Calculated). |
| Uniprot: | P41235 | Q14541 |
| RRID: | AB_2834237 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF0534, RRID:AB_2834237.
Fold/Unfold
FLJ39654; FRTS4; Hepatic nuclear factor 4 alpha; Hepatocyte nuclear factor 4 alpha; Hepatocyte nuclear factor 4; Hepatocyte nuclear factor 4-alpha; HNF 4 alpha; HNF 4; HNF-4-alpha; HNF4; HNF4A; HNF4A_HUMAN; HNF4a7; HNF4a8; HNF4a9; Hnf4alpha; HNF4alpha10/11/12; MODY 1; MODY; MODY1; NR2A1; NR2A21; Nuclear receptor subfamily 2 group A member 1; OTTHUMP00000031060; OTTHUMP00000031062; TCF 14; TCF; TCF-14; TCF14; Tcf4; Transcription factor 14, hepatic nuclear factor; Transcription factor 14; Transcription factor HNF 4; Transcription factor HNF-4; Transcription factor HNF4; Hepatocyte nuclear factor 4 gamma; Hepatocyte nuclear factor 4-gamma; HNF 4 gamma; HNF-4-gamma; Hnf4g; HNF4G_HUMAN; NR2A2; NR2A3; Nuclear receptor subfamily 2 group A member 2;
Immunogens
Expressed in pancreas, kidney, small intestine and testis. Weakly expressed in colon. Not expressed in liver, skeletal muscle, lung, placenta, brain, heart, peripheral blood, ovary, prostate, thymus and spleen.
- P41235 HNF4A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRLSKTLVDMDMADYSAALDPAYTTLEFENVQVLTMGNDTSPSEGTNLNAPNSLGVSALCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTRRSSYEDSSLPSINALLQAEVLSRQITSPVSGINGDIRAKKIASIADVCESMKEQLLVLVEWAKYIPAFCELPLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLLGNDYIVPRHCPELAEMSRVSIRILDELVLPFQELQIDDNEYAYLKAIIFFDPDAKGLSDPGKIKRLRSQVQVSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQMIEQIQFIKLFGMAKIDNLLQEMLLGGSPSDAPHAHHPLHPHLMQEHMGTNVIVANTMPTHLSNGQMCEWPRPRGQAATPETPQPSPPGGSGSEPYKLLPGAVATIVKPLSAIPQPTITKQEVI
- Q14541 HNF4G_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNTTDNGVNCLCAICGDRATGKHYGASSCDGCKGFFRRSIRKSHVYSCRFSRQCVVDKDKRNQCRYCRLRKCFRAGMKKEAVQNERDRISTRRSTFDGSNIPSINTLAQAEVRSRQISVSSPGSSTDINVKKIASIGDVCESMKQQLLVLVEWAKYIPAFCELPLDDQVALLRAHAGEHLLLGATKRSMMYKDILLLGNNYVIHRNSCEVEISRVANRVLDELVRPFQEIQIDDNEYACLKAIVFFDPDAKGLSDPVKIKNMRFQVQIGLEDYINDRQYDSRGRFGELLLLLPTLQSITWQMIEQIQFVKLFGMVKIDNLLQEMLLGGASNDGSHLHHPMHPHLSQDPLTGQTILLGPMSTLVHADQISTPETPLPSPPQGSGQEQYKIAANQASVISHQHLSKQKQL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P41235/Q14541 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y23 | Phosphorylation | P12931 (SRC) | Uniprot |
| Y72 | Phosphorylation | Uniprot | |
| S75 | Phosphorylation | Uniprot | |
| S76 | Phosphorylation | Uniprot | |
| S95 | Phosphorylation | Uniprot | |
| S99 | Phosphorylation | Uniprot | |
| K106 | Acetylation | Uniprot | |
| K108 | Acetylation | Uniprot | |
| K126 | Acetylation | Uniprot | |
| K127 | Acetylation | Uniprot | |
| S138 | Phosphorylation | Uniprot | |
| T139 | Phosphorylation | Uniprot | |
| S142 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S143 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| Y144 | Phosphorylation | Uniprot | |
| S147 | Phosphorylation | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| S151 | Phosphorylation | Uniprot | |
| T166 | Phosphorylation | Uniprot | |
| S167 | Phosphorylation | Q16539 (MAPK14) , Q15759 (MAPK11) | Uniprot |
| S170 | Phosphorylation | Uniprot | |
| S190 | Phosphorylation | Uniprot | |
| K234 | Ubiquitination | Uniprot | |
| S262 | Phosphorylation | Uniprot | |
| S265 | Phosphorylation | Uniprot | |
| Y286 | Phosphorylation | P12931 (SRC) | Uniprot |
| Y288 | Phosphorylation | P12931 (SRC) | Uniprot |
| S303 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| K307 | Ubiquitination | Uniprot | |
| K309 | Ubiquitination | Uniprot | |
| S313 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| S318 | Phosphorylation | Uniprot | |
| S378 | Phosphorylation | Uniprot | |
| T429 | Phosphorylation | Uniprot | |
| T432 | Phosphorylation | Uniprot | |
| S436 | Phosphorylation | Uniprot | |
| S441 | Phosphorylation | Uniprot | |
| S443 | Phosphorylation | Uniprot | |
| T455 | Phosphorylation | Uniprot | |
| K458 | Acetylation | Uniprot | |
| S461 | Phosphorylation | Uniprot | |
| T467 | Phosphorylation | Uniprot | |
| T469 | Phosphorylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y24 | Phosphorylation | Uniprot | |
| S27 | Phosphorylation | Uniprot | |
| S28 | Phosphorylation | Uniprot | |
| K58 | Acetylation | Uniprot | |
| S118 | Phosphorylation | Uniprot | |
| S121 | Phosphorylation | Uniprot | |
| S124 | Phosphorylation | Uniprot | |
| T126 | Phosphorylation | Uniprot |
Research Backgrounds
Transcriptional regulator which controls the expression of hepatic genes during the transition of endodermal cells to hepatic progenitor cells, facilitating the recruitment of RNA pol II to the promoters of target genes. Activates the transcription of CYP2C38 (By similarity). Represses the CLOCK-ARNTL/BMAL1 transcriptional activity and is essential for circadian rhythm maintenance and period regulation in the liver and colon cells.
Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution. Phosphorylation at Ser-313 by AMPK reduces the ability to form homodimers and bind DNA.
Acetylation at Lys-458 lowers transcriptional activation by about two-fold.
Nucleus.
Homodimerization is required for HNF4-alpha to bind to its recognition site. Interacts with CLOCK, ARNTL, CRY1, CRY2, PER1 and PER2. Interacts with NR0B2/SHP; the resulting heterodimer is transcriptionnally inactive. Interacts with DDX3X; this interaction disrupts the interaction between HNF4 and NR0B2 that forms inactive heterodimers and enhances the formation of active HNF4 homodimers.
The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
Belongs to the nuclear hormone receptor family. NR2 subfamily.
Transcription factor. Has a lower transcription activation potential than HNF4-alpha.
Nucleus.
Expressed in pancreas, kidney, small intestine and testis. Weakly expressed in colon. Not expressed in liver, skeletal muscle, lung, placenta, brain, heart, peripheral blood, ovary, prostate, thymus and spleen.
Belongs to the nuclear hormone receptor family. NR2 subfamily.
Research Fields
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Human Diseases > Endocrine and metabolic diseases > Maturity onset diabetes of the young.
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