ATP7B Antibody - #AF0410

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Product: ATP7B Antibody
Catalog: AF0410
Description: Rabbit polyclonal antibody to ATP7B
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit
Mol.Wt.: 157kDa; 157kD(Calculated).
Uniprot: P35670
RRID: AB_2834236

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(83%), Horse(83%), Sheep(83%), Rabbit(85%)
Clonality:
Polyclonal
Specificity:
ATP7B Antibody detects endogenous levels of total ATP7B.
RRID:
AB_2834236
Cite Format: Affinity Biosciences Cat# AF0410, RRID:AB_2834236.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ATP7B; ATP7B_HUMAN; ATPase, Cu(2+) transporting, beta polypeptide; ATPase, Cu++ transporting, beta polypeptide; Copper pump 2; Copper transporting ATPase 2; PWD; Toxic milk; tx; WC1; WD; Wilson disease associated protein; Wilson disease-associated protein; WND; WND/140 kDa;

Immunogens

Immunogen:
Uniprot:

P35670(ATP7B_HUMAN) >>Visit HPA database.

Gene(ID):
ATP7B(540)
Expression:
P35670 ATP7B_HUMAN:

Most abundant in liver and kidney and also found in brain. Isoform 2 is expressed in brain but not in liver. The cleaved form WND/140 kDa is found in liver cell lines and other tissues.

Description:
The protein encoded by this gene belongs to the family of P-type cation transport ATPases. This gene encodes a polypeptide that acts as a membrane copper-transport protein. Defects in this gene are the cause of Wilson disease (WD). WD is an autosomal recessive disorder of copper metabolism in which copper cannot be incorporated into ceruloplasmin in liver, and cannot be excreted from the liver into the bile. Alternatively spliced transcript variants encoding different isoforms have been identified.
Sequence:
MPEQERQITAREGASRKILSKLSLPTRAWEPAMKKSFAFDNVGYEGGLDGLGPSSQVATSTVRILGMTCQSCVKSIEDRISNLKGIISMKVSLEQGSATVKYVPSVVCLQQVCHQIGDMGFEASIAEGKAASWPSRSLPAQEAVVKLRVEGMTCQSCVSSIEGKVRKLQGVVRVKVSLSNQEAVITYQPYLIQPEDLRDHVNDMGFEAAIKSKVAPLSLGPIDIERLQSTNPKRPLSSANQNFNNSETLGHQGSHVVTLQLRIDGMHCKSCVLNIEENIGQLLGVQSIQVSLENKTAQVKYDPSCTSPVALQRAIEALPPGNFKVSLPDGAEGSGTDHRSSSSHSPGSPPRNQVQGTCSTTLIAIAGMTCASCVHSIEGMISQLEGVQQISVSLAEGTATVLYNPSVISPEELRAAIEDMGFEASVVSESCSTNPLGNHSAGNSMVQTTDGTPTSVQEVAPHTGRLPANHAPDILAKSPQSTRAVAPQKCFLQIKGMTCASCVSNIERNLQKEAGVLSVLVALMAGKAEIKYDPEVIQPLEIAQFIQDLGFEAAVMEDYAGSDGNIELTITGMTCASCVHNIESKLTRTNGITYASVALATSKALVKFDPEIIGPRDIIKIIEEIGFHASLAQRNPNAHHLDHKMEIKQWKKSFLCSLVFGIPVMALMIYMLIPSNEPHQSMVLDHNIIPGLSILNLIFFILCTFVQLLGGWYFYVQAYKSLRHRSANMDVLIVLATSIAYVYSLVILVVAVAEKAERSPVTFFDTPPMLFVFIALGRWLEHLAKSKTSEALAKLMSLQATEATVVTLGEDNLIIREEQVPMELVQRGDIVKVVPGGKFPVDGKVLEGNTMADESLITGEAMPVTKKPGSTVIAGSINAHGSVLIKATHVGNDTTLAQIVKLVEEAQMSKAPIQQLADRFSGYFVPFIIIMSTLTLVVWIVIGFIDFGVVQRYFPNPNKHISQTEVIIRFAFQTSITVLCIACPCSLGLATPTAVMVGTGVAAQNGILIKGGKPLEMAHKIKTVMFDKTGTITHGVPRVMRVLLLGDVATLPLRKVLAVVGTAEASSEHPLGVAVTKYCKEELGTETLGYCTDFQAVPGCGIGCKVSNVEGILAHSERPLSAPASHLNEAGSLPAEKDAVPQTFSVLIGNREWLRRNGLTISSDVSDAMTDHEMKGQTAILVAIDGVLCGMIAIADAVKQEAALAVHTLQSMGVDVVLITGDNRKTARAIATQVGINKVFAEVLPSHKVAKVQELQNKGKKVAMVGDGVNDSPALAQADMGVAIGTGTDVAIEAADVVLIRNDLLDVVASIHLSKRTVRRIRINLVLALIYNLVGIPIAAGVFMPIGIVLQPWMGSAAMAASSVSVVLSSLQLKCYKKPDLERYEAQAHGHMKPLTASQVSVHIGMDDRWRDSPRATPWDQVSYVSQVSLSSLTSDKPSRHSAAADDDGDKWSLLLNGRDEEQYI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
85
Horse
83
Bovine
83
Sheep
83
Chicken
73
Pig
0
Dog
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35670 As Substrate

Site PTM Type Enzyme
T9 Phosphorylation
S23 Phosphorylation
S132 Phosphorylation
S229 Phosphorylation
S340 Phosphorylation
S341 Phosphorylation
S345 Phosphorylation
S348 Phosphorylation
S478 Phosphorylation
S481 Phosphorylation
K489 Ubiquitination
S518 Phosphorylation
K607 Ubiquitination
K1028 Acetylation
K1028 Ubiquitination
T1050 Phosphorylation
T1076 Phosphorylation
Y1078 Phosphorylation
S1121 Phosphorylation
T1208 Phosphorylation
S1211 Phosphorylation
T1220 Phosphorylation
K1258 Acetylation
K1260 Acetylation
S1398 Phosphorylation
S1401 Phosphorylation
S1413 Phosphorylation
S1423 Phosphorylation
S1426 Phosphorylation
S1453 Phosphorylation

Research Backgrounds

Function:

Copper ion transmembrane transporter involved in the export of copper out of the cells. It is involved in copper homeostasis in the liver, where it ensures the efflux of copper from hepatocytes into the bile in response to copper overload.

PTMs:

Isoform 1 may be proteolytically cleaved at the N-terminus to produce the WND/140 kDa form.

Subcellular Location:

Golgi apparatus>trans-Golgi network membrane>Multi-pass membrane protein. Late endosome.
Note: Predominantly found in the trans-Golgi network (TGN). Localized in the trans-Golgi network under low copper conditions, redistributes to cytoplasmic vesicles when cells are exposed to elevated copper levels, and then recycles back to the trans-Golgi network when copper is removed (PubMed:10942420).

Golgi apparatus membrane>Multi-pass membrane protein.

Cytoplasm.

Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Most abundant in liver and kidney and also found in brain. Isoform 2 is expressed in brain but not in liver. The cleaved form WND/140 kDa is found in liver cell lines and other tissues.

Subunit Structure:

Monomer. Interacts with COMMD1/MURR1. Interacts with DCTN4, in a copper-dependent manner. Interacts with ATOX1. Interacts (via C-terminus) with ZBTB16/PLZF.

Family&Domains:

Each HMA domain can bind a copper ion, they are tightly packed and closely interact with each other. Wild-type ATP7B can usually be loaded with an average 5.5 copper atoms per molecule.

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.

Research Fields

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

References

1). Inhibition of ASIC1a-Mediated ERS Improves the Activation of HSCs and Copper Transport Under Copper Load. Frontiers in Pharmacology, 2021 (PubMed: 34135753) [IF=5.6]
2). Bioinformatics analysis and experimental validation of m6A and cuproptosis-related lncRNA NFE4 in clear cell renal cell carcinoma. Discover oncology, 2024 (PubMed: 38797784) [IF=2.2]

Application: WB    Species: Human    Sample: Caki-1 and OS-RC-2 cells

Figure. 9 Functional experiments against NFE4 in Caki-1 and OS-RC-2 cells. A NFE4 was highly expressed in tumor tissues. B The expression of NFE4 in renal cancer cells was measured using real-time quantitative reverse transcription-PCR. C Survival analysis of the high NFE4 group and low NFE4 group. D, E The mRNA expression of NFE4 in Caki-1 and OS-RC-2 cells was measured using real-time quantitative reverse transcription-PCR. F, G Cell Counting Kit-8 assays were used to detect the effect of NFE4 knockdown on cell proliferation in Caki-1 and OS-RC-2 cells. H qRT-PCR shows the expression and regulation of NFE4 in Caki-1 and OS-RC-2 cells treated with drugs that induce cuproptosis for 24 h (n = 3). CuCl2 (2mM), Elesclomol (20 nM), both CuCl2 (2mM) and Elesclomol (20 nM). I Western blotting was used to determine the protein levels of ATP7B and SLC31A1 in Caki-1 and OS-RC-2 cells. One-way ANOVA, NC was used as a control group. Data are shown as the mean ± standard deviation from three independent experiments and were compared to the respective si-NC group. ****P < 0.0001; ***P < 0.001; **P < 0.01 and *P < 0.05.

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