RPL31 Antibody - #AF0854
(3)
| Product: | RPL31 Antibody |
| Catalog: | AF0854 |
| Description: | Rabbit polyclonal antibody to RPL31 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 14kDa; 14kD(Calculated). |
| Uniprot: | P62899 |
| RRID: | AB_2834226 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:1000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
RPL31 Antibody detects endogenous levels of total RPL31.
RRID:
AB_2834226
Cite Format: Affinity Biosciences Cat# AF0854, RRID:AB_2834226.
Cite Format: Affinity Biosciences Cat# AF0854, RRID:AB_2834226.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
60S ribosomal protein L31; L31; MGC88191; OTTHUMP00000202933; OTTHUMP00000202940; OTTHUMP00000202941; OTTHUMP00000202942; OTTHUMP00000202943; Ribosomal protein L31; RL31_HUMAN; rpl31;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Description:
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L31E family of ribosomal proteins. It is located in the cytoplasm. Higher levels of expression of this gene in familial adenomatous polyps compared to matched normal tissues have been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Sequence:
- P62899 RL31_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAPAKKGGEKKKGRSAINEVVTREYTINIHKRIHGVGFKKRAPRALKEIRKFAMKEMGTPDVRIDTRLNKAVWAKGIRNVPYRIRVRLSRKRNEDEDSPNKLYTLVTYVPVTTFKNLQTVNVDEN
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P62899 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| R14 | Methylation | Uniprot | |
| S15 | Phosphorylation | Uniprot | |
| Y25 | Phosphorylation | Uniprot | |
| T26 | Phosphorylation | Uniprot | |
| K31 | Methylation | Uniprot | |
| K31 | Ubiquitination | Uniprot | |
| K39 | Acetylation | Uniprot | |
| K39 | Methylation | Uniprot | |
| K39 | Ubiquitination | Uniprot | |
| K55 | Acetylation | Uniprot | |
| K55 | Ubiquitination | Uniprot | |
| R63 | Methylation | Uniprot | |
| K70 | Ubiquitination | Uniprot | |
| K75 | Acetylation | Uniprot | |
| K75 | Ubiquitination | Uniprot | |
| S89 | Phosphorylation | Uniprot | |
| S98 | Phosphorylation | Uniprot | |
| K101 | Ubiquitination | Uniprot | |
| Y103 | Phosphorylation | Uniprot | |
| T104 | Phosphorylation | Uniprot | |
| T107 | Phosphorylation | Uniprot | |
| Y108 | Phosphorylation | Uniprot | |
| T113 | Phosphorylation | Uniprot | |
| K115 | Methylation | Uniprot | |
| K115 | Ubiquitination | Uniprot | |
| T119 | Phosphorylation | Uniprot |
Research Backgrounds
Family&Domains:
Belongs to the eukaryotic ribosomal protein eL31 family.
Research Fields
· Genetic Information Processing > Translation > Ribosome.
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