DAK Antibody - #AF0661
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF0661, RRID:AB_2834214.
Fold/Unfold
ATP dependent dihydroxyacetone kinase; Bifunctional ATP dependent dihydroxyacetone kinase/FAD AMP lyase (cyclizing); DAK; DHA kinase; Dha kinase/FMN cyclase; DHAK_HUMAN; Dihydroxyacetone kinase; Dihydroxyacetone kinase 2; Dihydroxyacetone kinase 2 homolog (S. cerevisiae); Dihydroxyacetone kinase 2 homolog (yeast); FAD AMP lyase (cyclic FMN forming); FAD AMP lyase (cyclizing); FAD-AMP lyase (cyclic FMN forming); FAD-AMP lyase (cyclizing); FMN cyclase; Glycerone kinase;
Immunogens
- Q3LXA3 TKFC_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTSKKLVNSVAGCADDALAGLVACNPNLQLLQGHRVALRSDLDSLKGRVALLSGGGSGHEPAHAGFIGKGMLTGVIAGAVFTSPAVGSILAAIRAVAQAGTVGTLLIVKNYTGDRLNFGLAREQARAEGIPVEMVVIGDDSAFTVLKKAGRRGLCGTVLIHKVAGALAEAGVGLEEIAKQVNVVAKAMGTLGVSLSSCSVPGSKPTFELSADEVELGLGIHGEAGVRRIKMATADEIVKLMLDHMTNTTNASHVPVQPGSSVVMMVNNLGGLSFLELGIIADATVRSLEGRGVKIARALVGTFMSALEMPGISLTLLLVDEPLLKLIDAETTAAAWPNVAAVSITGRKRSRVAPAEPQEAPDSTAAGGSASKRMALVLERVCSTLLGLEEHLNALDRAAGDGDCGTTHSRAARAIQEWLKEGPPPASPAQLLSKLSVLLLEKMGGSSGALYGLFLTAAAQPLKAKTSLPAWSAAMDAGLEAMQKYGKAAPGDRTMLDSLWAAGQELQAWKSPGADLLQVLTKAVKSAEAAAEATKNMEAGAGRASYISSARLEQPDPGAVAAAAILRAILEVLQS
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q3LXA3 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K46 | Ubiquitination | Uniprot | |
| R115 | Methylation | Uniprot | |
| S343 | Phosphorylation | Uniprot | |
| S350 | Phosphorylation | Uniprot | |
| S363 | Phosphorylation | Uniprot | |
| S369 | Phosphorylation | Uniprot | |
| S371 | Phosphorylation | Uniprot | |
| K372 | Acetylation | Uniprot | |
| K372 | Ubiquitination | Uniprot | |
| C404 | S-Nitrosylation | Uniprot | |
| K420 | Ubiquitination | Uniprot | |
| S427 | Phosphorylation | Uniprot | |
| S436 | Phosphorylation | Uniprot | |
| K465 | Ubiquitination | Uniprot | |
| S511 | Phosphorylation | Uniprot | |
| K522 | Ubiquitination | Uniprot | |
| K525 | Ubiquitination | Uniprot | |
| S545 | Phosphorylation | Uniprot | |
| Y546 | Phosphorylation | Uniprot | |
| S575 | Phosphorylation | Uniprot |
Research Backgrounds
Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde, and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate. Represses IFIH1-mediated cellular antiviral response.
Detected in erythrocytes (at protein level).
Homodimer (By similarity). Interacts with IFIH1 (via the CARD domains), the interaction is inhibited by viral infection.
DhaK and DhaL domains have differential roles, individually DhaK is inactive and DhaL displays cyclase but not kinase activity.
Belongs to the dihydroxyacetone kinase (DAK) family.
Research Fields
· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.
· Metabolism > Lipid metabolism > Glycerolipid metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway. (View pathway)
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