AAK1 Antibody - #AF0685
| Product: | AAK1 Antibody |
| Catalog: | AF0685 |
| Description: | Rabbit polyclonal antibody to AAK1 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse, Rat, Monkey |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 94kDa; 104kD(Calculated). |
| Uniprot: | Q2M2I8 |
| RRID: | AB_2834213 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF0685, RRID:AB_2834213.
Fold/Unfold
AAK 1; Aak1; AAK1_HUMAN; Adaptor associated kinase 1; Adaptor-associated kinase 1; AP2 associated kinase 1; AP2 associated protein kinase 1; AP2-associated protein kinase 1; DKFZp686F03202; DKFZp686K16132; FLJ23712; FLJ25931; FLJ31060; FLJ42882; FLJ45252; KIAA1048; MGC138170; MGC164568; MGC164570;
Immunogens
Detected in brain, heart and liver. Isoform 1 is the predominant isoform in brain.
- Q2M2I8 AAK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINNVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKIITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPAKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKPQAPPSQPLPQTQAKQPQAPPTPQQTPSTQAQGLPAQAQATPQHQQQLFLKQQQQQQQPPPAQQQPAGTFYQQQQAQTQQFQAVHPATQKPAIAQFPVVSQGGSQQQLMQNFYQQQQQQQQQQQQQQLATALHQQQLMTQQAALQQKPTMAAGQQPQPQPAAAPQPAPAQEPAIQAPVRQQPKVQTTPPPAVQGQKVGSLTPPSSPKTQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRTSQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKHPEKLGGSAESLIPGFQSTQGDAFATTSFSAGTAEKRKGGQTVDSGLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLLDCSLLSNPTTDLLEEFAPTAISAPVHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGGHSRNSSGSSESSLPNLARSLLLVDQLIDL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q2M2I8 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S7 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| S21 | Phosphorylation | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| T27 | Phosphorylation | Uniprot | |
| Y34 | Phosphorylation | Uniprot | |
| K88 | Ubiquitination | Uniprot | |
| K169 | Ubiquitination | Uniprot | |
| T170 | Phosphorylation | Uniprot | |
| K178 | Ubiquitination | Uniprot | |
| K201 | Ubiquitination | Uniprot | |
| T207 | Phosphorylation | Uniprot | |
| K219 | Ubiquitination | Uniprot | |
| T221 | Phosphorylation | Uniprot | |
| T222 | Phosphorylation | Uniprot | |
| Y234 | Phosphorylation | Uniprot | |
| S235 | Phosphorylation | Uniprot | |
| Y253 | Phosphorylation | Uniprot | |
| Y292 | Phosphorylation | Uniprot | |
| K317 | Methylation | Uniprot | |
| S327 | Phosphorylation | Uniprot | |
| T347 | Phosphorylation | Uniprot | |
| T354 | Phosphorylation | Uniprot | |
| T359 | Phosphorylation | Uniprot | |
| T362 | Phosphorylation | Uniprot | |
| T389 | Phosphorylation | P06493 (CDK1) | Uniprot |
| R391 | Methylation | Uniprot | |
| T441 | Phosphorylation | Uniprot | |
| T605 | Phosphorylation | Uniprot | |
| T606 | Phosphorylation | Uniprot | |
| S618 | Phosphorylation | Uniprot | |
| T620 | Phosphorylation | Uniprot | |
| S623 | Phosphorylation | Uniprot | |
| S624 | Phosphorylation | Uniprot | |
| T627 | Phosphorylation | Uniprot | |
| S637 | Phosphorylation | Q15208 (STK38) | Uniprot |
| T640 | Phosphorylation | Uniprot | |
| S642 | Phosphorylation | Uniprot | |
| S650 | Phosphorylation | Uniprot | |
| S652 | Phosphorylation | Uniprot | |
| T653 | Phosphorylation | Uniprot | |
| S664 | Phosphorylation | Uniprot | |
| S668 | Phosphorylation | Uniprot | |
| S670 | Phosphorylation | Uniprot | |
| T672 | Phosphorylation | Uniprot | |
| T673 | Phosphorylation | Uniprot | |
| T674 | Phosphorylation | Uniprot | |
| S676 | Phosphorylation | Uniprot | |
| S678 | Phosphorylation | Uniprot | |
| T681 | Phosphorylation | Uniprot | |
| S682 | Phosphorylation | Uniprot | |
| Y687 | Phosphorylation | Uniprot | |
| S690 | Phosphorylation | Uniprot | |
| K705 | Acetylation | Uniprot | |
| S731 | Phosphorylation | Uniprot | |
| S797 | Phosphorylation | Uniprot | |
| S846 | Phosphorylation | Uniprot | |
| T848 | Phosphorylation | Uniprot | |
| T852 | Phosphorylation | Uniprot | |
| S951 | Phosphorylation | Uniprot |
PTMs - Q2M2I8 As Enzyme
Research Backgrounds
Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity.
Autophosphorylated.
Cell membrane>Peripheral membrane protein. Membrane>Clathrin-coated pit. Cell junction>Synapse>Presynapse.
Note: Active when found in clathrin-coated pits at the plasma membrane. In neuronal cells, enriched at presynaptic terminals. In non-neuronal cells, enriched at leading edge of migrating cells.
Detected in brain, heart and liver. Isoform 1 is the predominant isoform in brain.
Interacts with alpha-adaptin, AP-2, clathrin, NUMB and EPS15 isoform 2. Interacts with membrane-bound activated NOTCH1 but not with the inactive full-length form of NOTCH1. Preferentially interacts with monoubiquitinated activated NOTCH1 compared to the non-ubiquitinated form.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
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