Product: AAK1 Antibody
Catalog: AF0685
Description: Rabbit polyclonal antibody to AAK1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 94kDa; 104kD(Calculated).
Uniprot: Q2M2I8
RRID: AB_2834213

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
AAK1 Antibody detects endogenous levels of total AAK1.
RRID:
AB_2834213
Cite Format: Affinity Biosciences Cat# AF0685, RRID:AB_2834213.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AAK 1; Aak1; AAK1_HUMAN; Adaptor associated kinase 1; Adaptor-associated kinase 1; AP2 associated kinase 1; AP2 associated protein kinase 1; AP2-associated protein kinase 1; DKFZp686F03202; DKFZp686K16132; FLJ23712; FLJ25931; FLJ31060; FLJ42882; FLJ45252; KIAA1048; MGC138170; MGC164568; MGC164570;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q2M2I8 AAK1_HUMAN:

Detected in brain, heart and liver. Isoform 1 is the predominant isoform in brain.

Description:
AAK1 is an NAK kinase. Plays a regulatory role in clathrin-mediated endocytosis, including the regulation of fatty acid synthesis and cholesterol synthesis.
Sequence:
MKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINNVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKIITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPAKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKPQAPPSQPLPQTQAKQPQAPPTPQQTPSTQAQGLPAQAQATPQHQQQLFLKQQQQQQQPPPAQQQPAGTFYQQQQAQTQQFQAVHPATQKPAIAQFPVVSQGGSQQQLMQNFYQQQQQQQQQQQQQQLATALHQQQLMTQQAALQQKPTMAAGQQPQPQPAAAPQPAPAQEPAIQAPVRQQPKVQTTPPPAVQGQKVGSLTPPSSPKTQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRTSQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKHPEKLGGSAESLIPGFQSTQGDAFATTSFSAGTAEKRKGGQTVDSGLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLLDCSLLSNPTTDLLEEFAPTAISAPVHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGGHSRNSSGSSESSLPNLARSLLLVDQLIDL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q2M2I8 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S7 Phosphorylation
S14 Phosphorylation
S18 Phosphorylation
S20 Phosphorylation
S21 Phosphorylation
S26 Phosphorylation
T27 Phosphorylation
Y34 Phosphorylation
K88 Ubiquitination
K169 Ubiquitination
T170 Phosphorylation
K178 Ubiquitination
K201 Ubiquitination
T207 Phosphorylation
K219 Ubiquitination
T221 Phosphorylation
T222 Phosphorylation
Y234 Phosphorylation
S235 Phosphorylation
Y253 Phosphorylation
Y292 Phosphorylation
K317 Methylation
S327 Phosphorylation
T347 Phosphorylation
T354 Phosphorylation
T359 Phosphorylation
T362 Phosphorylation
T389 Phosphorylation P06493 (CDK1)
R391 Methylation
T441 Phosphorylation
T605 Phosphorylation
T606 Phosphorylation
S618 Phosphorylation
T620 Phosphorylation
S623 Phosphorylation
S624 Phosphorylation
T627 Phosphorylation
S637 Phosphorylation Q15208 (STK38)
T640 Phosphorylation
S642 Phosphorylation
S650 Phosphorylation
S652 Phosphorylation
T653 Phosphorylation
S664 Phosphorylation
S668 Phosphorylation
S670 Phosphorylation
T672 Phosphorylation
T673 Phosphorylation
T674 Phosphorylation
S676 Phosphorylation
S678 Phosphorylation
T681 Phosphorylation
S682 Phosphorylation
Y687 Phosphorylation
S690 Phosphorylation
K705 Acetylation
S731 Phosphorylation
S797 Phosphorylation
S846 Phosphorylation
T848 Phosphorylation
T852 Phosphorylation
S951 Phosphorylation

PTMs - Q2M2I8 As Enzyme

Substrate Site Source
P49757 (NUMB) T102 Uniprot
Q96CW1 (AP2M1) T156 Uniprot
Q9BXS5 (AP1M1) T144 Uniprot

Research Backgrounds

Function:

Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity.

PTMs:

Autophosphorylated.

Subcellular Location:

Cell membrane>Peripheral membrane protein. Membrane>Clathrin-coated pit. Cell junction>Synapse>Presynapse.
Note: Active when found in clathrin-coated pits at the plasma membrane. In neuronal cells, enriched at presynaptic terminals. In non-neuronal cells, enriched at leading edge of migrating cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in brain, heart and liver. Isoform 1 is the predominant isoform in brain.

Subunit Structure:

Interacts with alpha-adaptin, AP-2, clathrin, NUMB and EPS15 isoform 2. Interacts with membrane-bound activated NOTCH1 but not with the inactive full-length form of NOTCH1. Preferentially interacts with monoubiquitinated activated NOTCH1 compared to the non-ubiquitinated form.

Family&Domains:

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Restrictive clause

 

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