CBX6 Antibody - #AF0411

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Product: CBX6 Antibody
Catalog: AF0411
Description: Rabbit polyclonal antibody to CBX6
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Xenopus
Mol.Wt.: 44kDa; 44kD(Calculated).
Uniprot: O95503
RRID: AB_2834202

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
CBX6 Antibody detects endogenous levels of total CBX6.
RRID:
AB_2834202
Cite Format: Affinity Biosciences Cat# AF0411, RRID:AB_2834202.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cbx 6; Cbx6; CBX6_HUMAN; Chromobox homolog 6; Chromobox protein homolog 6;

Immunogens

Immunogen:
Uniprot:

O95503(CBX6_HUMAN) >>Visit HPA database.

Gene(ID):
CBX6(23466)
Description:
CBX6 Involved in maintaining the transcriptionally repressive state of genes. Modifies chromatin, rendering it heritably changed in its expressibility (Probable). Component of the chromatin-associated polycomb complex (PcG). Note: This description may include information from UniProtKB.
Sequence:
MELSAVGERVFAAESIIKRRIRKGRIEYLVKWKGWAIKYSTWEPEENILDSRLIAAFEQKERERELYGPKKRGPKPKTFLLKARAQAEALRISDVHFSVKPSASASSPKLHSSAAVHRLKKDIRRCHRMSRRPLPRPDPQGGSPGLRPPISPFSETVRIINRKVKPREPKRNRIILNLKVIDKGAGGGGAGQGAGALARPKVPSRNRVIGKSKKFSESVLRTQIRHMKFGAFALYKPPPAPLVAPSPGKAEASAPGPGLLLAAPAAPYDARSSGSSGCPSPTPQSSDPDDTPPKLLPETVSPSAPSWREPEVLDLSLPPESAATSKRAPPEVTAAAGPAPPTAPEPAGASSEPEAGDWRPEMSPCSNVVVTDVTSNLLTVTIKEFCNPEDFEKVAAGVAGAAGGGGSIGASK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Xenopus
100
Chicken
67
Horse
0
Sheep
0
Dog
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95503 As Substrate

Site PTM Type Enzyme
K18 Ubiquitination
K33 Ubiquitination
K38 Ubiquitination
Y39 Phosphorylation
K60 Ubiquitination
K82 Ubiquitination
K100 Acetylation
K100 Ubiquitination
S106 Phosphorylation
S107 Phosphorylation
S130 Phosphorylation
S143 Phosphorylation
S151 Phosphorylation
T156 Phosphorylation
K179 Ubiquitination
K183 Ubiquitination
K214 Ubiquitination
K236 Ubiquitination
S246 Phosphorylation
S280 Phosphorylation
T282 Phosphorylation
S285 Phosphorylation
S301 Phosphorylation
S303 Phosphorylation

Research Backgrounds

Function:

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Possibly contributes to the target selectivity of the PRC1 complex by binding specific regions of chromatin. Recruitment to chromatin might occur in an H3K27me3-independent fashion (By similarity). May have a PRC1-independent function in embryonic stem cells (By similarity).

Subcellular Location:

Nucleus. Chromosome.
Note: Uniformely distributed in the nucleoplasm (PubMed:18927235). Localizes to the inactivated X chromosome in females (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of a PRC1-like complex. Distinct PRC1-like core complexes are composed of a RING1 subunit (RING1B or RING1A), one of the six PCGF proteins (PCGF1-6), one PHC protein (PHC1-3) and one of the CBX proteins (CBX2, CBX4, CBX6, CBX7 or CBX8). Interacts with PCGF1, PCGF2, PCGF3, BMI1, PCGF5, PCGF6, RING1 and RNF2. May interact with HIST2H3A and HIST1H3A. Interacts (via chromodomain) with single-stranded RNA (ssRNA) (By similarity).

References

1). Identification of the Prognostic Biomarkers CBX6 and CBX7 in Bladder Cancer. Diagnostics (Basel, Switzerland), 2023 (PubMed: 37189494) [IF=3.6]

Application: WB    Species: Human    Sample: BLCA cell and tissue

Figure 11. Validation of CBX family protein expression levels in BLCA cell and tissue samples. (A) The expression levels of CBX family proteins were detected by Western blotting in normal urothelial cell lines (SV-HUC-1) and BLCA cell lines (5637 and T24) (n = 3). (B–I) Quantitative results of (A). (J,K) Expression levels of CBX6, CD25, and CD86 in BLCA tumor tissue samples analyzed by immunohistochemistry (IHC). (L,M) Expression level of CBX7, KIT, and CD14 analyzed by IHC in BLCA tumor tissue samples. * p < 0.05; ** p < 0.01; *** p < 0.001.

Application: IF/ICC    Species: Human    Sample: BLCA cell and tissue

Figure 11. Validation of CBX family protein expression levels in BLCA cell and tissue samples. (A) The expression levels of CBX family proteins were detected by Western blotting in normal urothelial cell lines (SV-HUC-1) and BLCA cell lines (5637 and T24) (n = 3). (B–I) Quantitative results of (A). (J,K) Expression levels of CBX6, CD25, and CD86 in BLCA tumor tissue samples analyzed by immunohistochemistry (IHC). (L,M) Expression level of CBX7, KIT, and CD14 analyzed by IHC in BLCA tumor tissue samples. * p < 0.05; ** p < 0.01; *** p < 0.001.

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