ZNF265 Antibody - #AF0753

Product: ZNF265 Antibody
Catalog: AF0753
Description: Rabbit polyclonal antibody to ZNF265
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 34kDa; 37kD(Calculated).
Uniprot: O95218
RRID: AB_2834194

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
ZNF265 Antibody detects endogenous levels of total ZNF265.
RRID:
AB_2834194
Cite Format: Affinity Biosciences Cat# AF0753, RRID:AB_2834194.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ZRANB 2; DKFZp686J1831; DKFZp686N09117; FLJ41119; OTTHUMP00000011240; OTTHUMP00000011241; Zinc finger protein 265; Zinc finger RAN binding domain containing 2; Zinc finger Ran binding domain containing protein 2; Zinc finger splicing; ZIS; ZIS1; ZIS2; ZNF265;

Immunogens

Immunogen:
Uniprot:

O95218(ZRAB2_HUMAN) >>Visit HPA database.

Gene(ID):
ZRANB2(9406)
Description:
ZNF265 Splice factor required for alternative splicing of TRA2B/SFRS10 transcripts. May interfere with constitutive 5'- splice site selection. Belongs to the ZRANB2 family. Interacts with the C-terminal half of SNRNP70, the Arg/Ser-rich domain of XE7 as well as with U2AF1 and CLK1. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Sequence:
MSTKNFRVSDGDWICPDKKCGNVNFARRTSCNRCGREKTTEAKMMKAGGTEIGKTLAEKSRGLFSANDWQCKTCSNVNWARRSECNMCNTPKYAKLEERTGYGGGFNERENVEYIEREESDGEYDEFGRKKKKYRGKAVGPASILKEVEDKESEGEEEDEDEDLSKYKLDEDEDEDDADLSKYNLDASEEEDSNKKKSNRRSRSKSRSSHSRSSSRSSSPSSSRSRSRSRSRSSSSSQSRSRSSSRERSRSRGSKSRSSSRSHRGSSSPRKRSYSSSSSSPERNRKRSRSRSSSSGDRKKRRTRSRSPERRHRSSSGSSHSGSRSSSKKK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95218 As Substrate

Site PTM Type Enzyme
S9 Phosphorylation
T50 Phosphorylation
K54 Acetylation
K54 Ubiquitination
K59 Acetylation
K59 Ubiquitination
R61 Methylation
S65 Phosphorylation
S83 Phosphorylation
T90 Phosphorylation
K92 Acetylation
Y93 Phosphorylation
K95 Sumoylation
K95 Ubiquitination
Y102 Phosphorylation
Y114 Phosphorylation
S120 Phosphorylation
Y124 Phosphorylation
K137 Methylation
S143 Phosphorylation
S153 Phosphorylation
S165 Phosphorylation
K168 Ubiquitination
S181 Phosphorylation
K182 Ubiquitination
Y183 Phosphorylation
S188 Phosphorylation
S193 Phosphorylation
S206 Phosphorylation
S208 Phosphorylation
S213 Phosphorylation
S217 Phosphorylation
S218 Phosphorylation
S221 Phosphorylation
S249 Phosphorylation
S273 Phosphorylation
S275 Phosphorylation
S279 Phosphorylation
S280 Phosphorylation
S288 Phosphorylation
S290 Phosphorylation
T303 Phosphorylation
S305 Phosphorylation
S307 Phosphorylation
S318 Phosphorylation
S323 Phosphorylation
S326 Phosphorylation

PTMs - O95218 As Enzyme

Substrate Site Source
O95218-2 (ZRANB2) S188 Uniprot

Research Backgrounds

Function:

Splice factor required for alternative splicing of TRA2B/SFRS10 transcripts. May interfere with constitutive 5'-splice site selection.

PTMs:

Isoform 2 is phosphorylated on Ser-310 upon DNA damage, probably by ATM or ATR.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with the C-terminal half of SNRNP70, the Arg/Ser-rich domain of AKAP17A as well as with U2AF1 and CLK1.

Family&Domains:

The RanBP2-type zinc fingers mediate binding to RNA.

Belongs to the ZRANB2 family.

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