TACC1 Antibody - #AF0456

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Product: TACC1 Antibody
Catalog: AF0456
Description: Rabbit polyclonal antibody to TACC1
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 87kDa; 88kD(Calculated).
Uniprot: O75410
RRID: AB_2834182

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
TACC1 Antibody detects endogenous levels of total TACC1.
RRID:
AB_2834182
Cite Format: Affinity Biosciences Cat# AF0456, RRID:AB_2834182.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp686K18126; Ga 55; Ga55; Gastric cancer antigen Ga 55; Gastric cancer antigen Ga55; KIAA1103; TACC 1; Tacc1; TACC1_HUMAN; Taxin 1; Taxin-1; Taxin1; Transforming acidic coiled coil containing protein 1; Transforming acidic coiled-coil-containing protein 1;

Immunogens

Immunogen:
Uniprot:

O75410(TACC1_HUMAN) >>Visit HPA database.

Gene(ID):
TACC1(6867)
Expression:
O75410 TACC1_HUMAN:

Isoform 1, isoform 3 and isoform 5 are ubiquitous. Isoform 2 is strongly expressed in the brain, weakly detectable in lung and colon, and overexpressed in gastric cancer. Isoform 4 is not detected in normal tissues, but strong expression was found in gastric cancer tissues. Down-regulated in a subset of cases of breast cancer.

Description:
Likely involved in the processes that promote cell division prior to the formation of differentiated tissues. Interacts with KIAA0097/CH-TOG and with the oncogenic transcription factor YEATS4.Interacts with the Aurora kinases A and B (STK6 and AURKB).Interacts with LSM7, TDRD7 and SNRPG.Interacts with GCN5L2 and PCAF. 8 isoforms of the human protein are produced by alternative splicing. Protein Type: Unknown function
Sequence:
MAFSPWQILSPVQWAKWTWSAVRGGAAGEDEAGGPEGDPEEEDSQAETKSLSFSSDSEGNFETPEAETPIRSPFKESCDPSLGLAGPGAKSQESQEADEQLVAEVVEKCSSKTCSKPSENEVPQQAIDSHSVKNFREEPEHDFSKISIVRPFSIETKDSTDISAVLGTKAAHGCVTAVSGKALPSSPPDALQDEAMTEGSMGVTLEASAEADLKAGNSCPELVPSRRSKLRKPKPVPLRKKAIGGEFSDTNAAVEGTPLPKASYHFSPEELDENTSPLLGDARFQKSPPDLKETPGTLSSDTNDSGVELGEESRSSPLKLEFDFTEDTGNIEARKALPRKLGRKLGSTLTPKIQKDGISKSAGLEQPTDPVARDGPLSQTSSKPDPSQWESPSFNPFGSHSVLQNSPPLSSEGSYHFDPDNFDESMDPFKPTTTLTSSDFCSPTGNHVNEILESPKKAKSRLITSGCKVKKHETQSLALDACSRDEGAVISQISDISNRDGHATDEEKLASTSCGQKSAGAEVKGEPEEDLEYFECSNVPVSTINHAFSSSEAGIEKETCQKMEEDGSTVLGLLESSAEKAPVSVSCGGESPLDGICLSESDKTAVLTLIREEIITKEIEANEWKKKYEETRQEVLEMRKIVAEYEKTIAQMIEDEQRTSMTSQKSFQQLTMEKEQALADLNSVERSLSDLFRRYENLKGVLEGFKKNEEALKKCAQDYLARVKQEEQRYQALKIHAEEKLDKANEEIAQVRTKAKAESAALHAGLRKEQMKVESLERALQQKNQEIEELTKICDELIAKLGKTD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75410 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S4 Phosphorylation
S10 Phosphorylation
S44 Phosphorylation
T48 Phosphorylation
S50 Phosphorylation
S52 Phosphorylation
S54 Phosphorylation
S55 Phosphorylation
S57 Phosphorylation
T63 Phosphorylation
T68 Phosphorylation
K75 Methylation
K75 Ubiquitination
S94 Phosphorylation
S131 Phosphorylation
S147 Phosphorylation
S153 Phosphorylation
T168 Phosphorylation
K169 Ubiquitination
S179 Phosphorylation
S218 Phosphorylation
S225 Phosphorylation
S228 Phosphorylation Q9UQB9 (AURKC)
K234 Acetylation
K240 Acetylation
S248 Phosphorylation
T257 Phosphorylation
Y264 Phosphorylation
S267 Phosphorylation
T275 Phosphorylation
S276 Phosphorylation
K286 Ubiquitination
S287 Phosphorylation
S305 Phosphorylation
S347 Phosphorylation
T350 Phosphorylation
K360 Ubiquitination
S361 Phosphorylation
S454 Phosphorylation
K468 Ubiquitination
K471 Ubiquitination
T504 Phosphorylation
K517 Ubiquitination
Y533 Phosphorylation
S591 Phosphorylation
T616 Phosphorylation
K617 Ubiquitination
K640 Acetylation
K640 Ubiquitination
K647 Acetylation
K647 Ubiquitination
K665 Ubiquitination
S666 Phosphorylation
S687 Phosphorylation
K714 Ubiquitination
Y730 Phosphorylation
K734 Ubiquitination
K754 Acetylation
K756 Ubiquitination
S759 Phosphorylation
K783 Ubiquitination
K792 Ubiquitination
K800 Ubiquitination

Research Backgrounds

Function:

Involved in transcription regulation induced by nuclear receptors, including in T3 thyroid hormone and all-trans retinoic acid pathways. Might promote the nuclear localization of the receptors. Likely involved in the processes that promote cell division prior to the formation of differentiated tissues.

PTMs:

Isoform 1 is heavily phosphorylated; isoform 6 is not.

Subcellular Location:

Cytoplasm. Nucleus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Midbody.
Note: Nucleus during interphase. Weakly concentrated at centrosomes during mitosis and colocalizes with AURKC at the midbody during cytokinesis.

Membrane>Lipid-anchor.

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 1, isoform 3 and isoform 5 are ubiquitous. Isoform 2 is strongly expressed in the brain, weakly detectable in lung and colon, and overexpressed in gastric cancer. Isoform 4 is not detected in normal tissues, but strong expression was found in gastric cancer tissues. Down-regulated in a subset of cases of breast cancer.

Subunit Structure:

Interacts with KIAA0097/CH-TOG and with the oncogenic transcription factor YEATS4. Interacts with AURKA, AURKB and AURKC. Interacts with LSM7, TDRD7 and SNRPG. Interacts with GCN5L2 and PCAF. Interacts with the thyroid hormone receptors THRB and THRA, predominantly with isoform alpha-2. The interaction with THRA isoform alpha-1 and THRB is decreased in the presence of thyroid hormone T3. Also interacts with other nuclear receptors, including ESR1, NR3C1, PPARG, RARA and RXRA, preferentially in the absence of their hormonal ligands.

Family&Domains:

Belongs to the TACC family.

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