PFDN1 Antibody | Affinity Biosciences

Product:	PFDN1 Antibody
Catalog:	AF0748
Description:	Rabbit polyclonal antibody to PFDN1
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.:	14kDa; 14kD(Calculated).
Uniprot:	O60925
RRID:	AB_2834180

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(82%), Xenopus(91%)

Clonality:

Polyclonal

Specificity:

PFDN1 Antibody detects endogenous levels of total PFDN1.

RRID:

AB_2834180
Cite Format: Affinity Biosciences Cat# AF0748, RRID:AB_2834180.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

PDF; PFD1; PFD1_HUMAN; pfdn1; Prefoldin 1; Prefoldin subunit 1;

Immunogens

Immunogen:

Uniprot:

O60925(PFD1_HUMAN) >>Visit HPA database.

Gene(ID):

PFDN1(5201)

Description:

PFDN1 Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. Belongs to the prefoldin subunit beta family. Heterohexamer of two PFD-alpha type and four PFD-beta type subunits. Note: This description may include information from UniProtKB.

Sequence:

O60925 PFD1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAPVDLELKKAFTELQAKVIDTQQKVKLADIQIEQLNRTKKHAHLTDTEIMTLVDETNMYEGVGRMFILQSKEAIHSQLLEKQKIAEEKIKELEQKKSYLERSVKEAEDNIREMLMARRAQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Rabbit

100

Xenopus

Chicken

Dog

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O60925 As Substrate

O60925

Site	PTM Type	Source
A2	Acetylation	Uniprot
K10	Acetylation	Uniprot
K10	Ubiquitination	Uniprot
K11	Ubiquitination	Uniprot
K19	Ubiquitination	Uniprot
K28	Methylation	Uniprot
K28	Ubiquitination	Uniprot
Y61	Phosphorylation	Uniprot
S72	Phosphorylation	Uniprot
K83	Ubiquitination	Uniprot
S99	Phosphorylation	Uniprot
K106	Ubiquitination	Uniprot

Research Backgrounds

O60925_PFD1_HUMAN

Function:

Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.

Subunit Structure:

Heterohexamer of two PFD-alpha type and four PFD-beta type subunits.

Family&Domains:

Belongs to the prefoldin subunit beta family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

PFDN1 Antibody - #AF0748