MMP8 Antibody - #AF0219

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Product: MMP8 Antibody
Catalog: AF0219
Description: Rabbit polyclonal antibody to MMP8
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Horse
Mol.Wt.: 55kDa; 53kD(Calculated).
Uniprot: P22894
RRID: AB_2833349

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Horse(80%)
Clonality:
Polyclonal
Specificity:
MMP8 Antibody detects endogenous levels of total MMP8.
RRID:
AB_2833349
Cite Format: Affinity Biosciences Cat# AF0219, RRID:AB_2833349.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CLG 1; CLG1; Collagenase 1; Collagenase 1 neutrophil; HNC; Matrix metallopeptidase 8 (neutrophil collagenase); Matrix metalloprotease 8; Matrix metalloproteinase-8; MMP 8; MMP-8; Mmp8; MMP8_HUMAN; Neutrophil collagenase; PMNL CL; PMNL collagenase; PMNL-CL; PMNLCL;

Immunogens

Immunogen:
Uniprot:

P22894(MMP8_HUMAN) >>Visit HPA database.

Gene(ID):
MMP8(4317)
Expression:
P22894 MMP8_HUMAN:

Neutrophils.

Description:
MMP8 Can degrade fibrillar type I, II, and III collagens. Belongs to the peptidase M10A family.
Sequence:
MFSLKTLPFLLLLHVQISKAFPVSSKEKNTKTVQDYLEKFYQLPSNQYQSTRKNGTNVIVEKLKEMQRFFGLNVTGKPNEETLDMMKKPRCGVPDSGGFMLTPGNPKWERTNLTYRIRNYTPQLSEAEVERAIKDAFELWSVASPLIFTRISQGEADINIAFYQRDHGDNSPFDGPNGILAHAFQPGQGIGGDAHFDAEETWTNTSANYNLFLVAAHEFGHSLGLAHSSDPGALMYPNYAFRETSNYSLPQDDIDGIQAIYGLSSNPIQPTGPSTPKPCDPSLTFDAITTLRGEILFFKDRYFWRRHPQLQRVEMNFISLFWPSLPTGIQAAYEDFDRDLIFLFKGNQYWALSGYDILQGYPKDISNYGFPSSVQAIDAAVFYRSKTYFFVNDQFWRYDNQRQFMEPGYPKSISGAFPGIESKVDAVFQQEHFFHVFSGPRYYAFDLIAQRVTRVARGNKWLNCRYG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
80
Bovine
70
Sheep
70
Rabbit
70
Pig
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P22894 As Substrate

Site PTM Type Enzyme
T30 Phosphorylation
T32 Phosphorylation
Y36 Phosphorylation
Y383 Phosphorylation
S385 Phosphorylation
S422 Phosphorylation

Research Backgrounds

Function:

Can degrade fibrillar type I, II, and III collagens.

Subcellular Location:

Cytoplasmic granule. Secreted>Extracellular space>Extracellular matrix.
Note: Stored in intracellular granules.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Neutrophils.

Family&Domains:

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Belongs to the peptidase M10A family.

References

1). RPRM negatively regulates ATM levels through its nuclear translocation on irradiation mediated by CDK4/6 and IPO11. iScience, 2021 (PubMed: 36185355) [IF=5.8]

Application: IF/ICC    Species: Human    Sample: H460-RPRM cells

Figure 3 RPRM promotes the nuclear export and degradation of ATM (A and B) Kinetics of the change in the ATM levels of H460-NC/RPRM cells with and without irradiation in the presence of cycloheximide (CHX, 0.1 mg/mL) examined by immunoblotting. (∗, RPRM vs NC; #, RPRM + IR vs NC + IR; &, RPRM + IR vs RPRM). (C and D) Change in the ATM levels of H460-NC (N)/RPRM (R) cells at the indicated time points after 2 Gy X-irradiation with and without MG132 (10 μM) pre-treatment. (E) Change in the subcellular localization of ATM in RPRM−/− MEFs transfected with NC and RPRM vectors after 20 Gy X-irradiation and quantification of the ratios of cytoplasmic ATM fluorescence intensity to nuclear ATM fluorescence intensity. Scale bars, 10 μm. (F) Representative immunofluorescence images of ATM in H460-RPRM cells pre-treated with leptomycin B (LMB, 10 nM) for 1 h at different times after 2 Gy X-irradiation and quantification of the cytoplasmic/nuclear ATM fluorescence intensity ratios. Scale bar, 20 μm. (G) The effect of LMB pre-treatment on the total ATM levels of H460-NC/RPRM cells at different times after 2 Gy X-irradiation. (H) Effect of LMB on the micronucleus formation of H460-NC/RPRM cells irradiated with 2 Gy X-rays.

Application: IF/ICC    Species: Human    Sample: H460-RPRM cells

Figure 3 RPRM promotes the nuclear export and degradation of ATM (A and B) Kinetics of the change in the ATM levels of H460-NC/RPRM cells with and without irradiation in the presence of cycloheximide (CHX, 0.1 mg/mL) examined by immunoblotting. (∗, RPRM vs NC; #, RPRM + IR vs NC + IR; &, RPRM + IR vs RPRM). (C and D) Change in the ATM levels of H460-NC (N)/RPRM (R) cells at the indicated time points after 2 Gy X-irradiation with and without MG132 (10 μM) pre-treatment. (E) Change in the subcellular localization of ATM in RPRM−/− MEFs transfected with NC and RPRM vectors after 20 Gy X-irradiation and quantification of the ratios of cytoplasmic ATM fluorescence intensity to nuclear ATM fluorescence intensity. Scale bars, 10 μm. (F) Representative immunofluorescence images of ATM in H460-RPRM cells pre-treated with leptomycin B (LMB, 10 nM) for 1 h at different times after 2 Gy X-irradiation and quantification of the cytoplasmic/nuclear ATM fluorescence intensity ratios. Scale bar, 20 μm. (G) The effect of LMB pre-treatment on the total ATM levels of H460-NC/RPRM cells at different times after 2 Gy X-irradiation. (H) Effect of LMB on the micronucleus formation of H460-NC/RPRM cells irradiated with 2 Gy X-rays.

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