ACTG2 Antibody - #AF0520

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Product: ACTG2 Antibody
Catalog: AF0520
Description: Rabbit polyclonal antibody to ACTG2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 45kDa; 42kD(Calculated).
Uniprot: P63267
RRID: AB_2834123

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
ACTG2 Antibody detects endogenous levels of total ACTG2.
RRID:
AB_2834123
Cite Format: Affinity Biosciences Cat# AF0520, RRID:AB_2834123.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ACT; ACTA3; ACTE; ACTG2; ACTH_HUMAN; Actin; Actin gamma 2 smooth muscle enteric; Actin gamma enteric smooth muscle; Actin like protein; ACTL3; ACTSG; Alpha actin 3; Alpha-actin-3; Gamma 2 actin; Gamma-2-actin; gamma-enteric smooth muscle; Smooth muscle gamma actin; Smooth muscle gamma-actin;

Immunogens

Immunogen:
Uniprot:

P63267(ACTH_HUMAN) >>Visit HPA database.

Gene(ID):
ACTG2(72)
Description:
ACTG2 Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Belongs to the actin family. Note: This description may include information from UniProtKB.
Sequence:
MCEEETTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKPEYDEAGPSIVHRKCF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P63267 As Substrate

Site PTM Type Enzyme
E3 Acetylation
S34 Phosphorylation
K51 Acetylation
K51 Methylation
K51 Ubiquitination
S53 Phosphorylation
Y54 Phosphorylation
S61 Phosphorylation
K62 Acetylation
K62 Sumoylation
K62 Ubiquitination
T67 Phosphorylation
K69 Acetylation
K69 Methylation
K69 Ubiquitination
Y70 Phosphorylation
T78 Phosphorylation
K85 Acetylation
K85 Methylation
S90 Phosphorylation
Y92 Phosphorylation
R96 Methylation
K114 Acetylation
K114 Ubiquitination
T127 Phosphorylation
Y144 Phosphorylation
T150 Phosphorylation
S156 Phosphorylation
Y167 Phosphorylation
Y170 Phosphorylation
T187 Phosphorylation
Y189 Phosphorylation
K192 Acetylation
K192 Methylation
K192 Ubiquitination
T195 Phosphorylation
R197 Methylation
Y199 Phosphorylation
S200 Phosphorylation
T203 Phosphorylation
K214 Acetylation
K214 Ubiquitination
K216 Acetylation
K216 Methylation
Y219 Phosphorylation
S234 Phosphorylation
S235 Phosphorylation
S236 Phosphorylation
K239 Ubiquitination
S240 Phosphorylation
Y241 Phosphorylation
T250 Phosphorylation
T261 Phosphorylation
S266 Phosphorylation
T279 Phosphorylation
Y280 Phosphorylation
S282 Phosphorylation
K292 Ubiquitination
Y295 Phosphorylation
T304 Phosphorylation
T305 Phosphorylation
Y307 Phosphorylation
K316 Acetylation
K316 Sumoylation
K316 Ubiquitination
T319 Phosphorylation
S324 Phosphorylation
T325 Phosphorylation
K327 Acetylation
K327 Methylation
K327 Sumoylation
K327 Ubiquitination
K329 Acetylation
K329 Sumoylation
K329 Ubiquitination
K337 Sumoylation
S349 Phosphorylation
Y363 Phosphorylation

Research Backgrounds

Function:

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

PTMs:

Oxidation of Met-45 and Met-48 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promotes actin repolymerization.

Monomethylation at Lys-85 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.

Methylated at His-74 by SETD3.

(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-51 of one monomer and Glu-271 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners.

Subcellular Location:

Cytoplasm>Cytoskeleton.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.

Family&Domains:

Belongs to the actin family.

Research Fields

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

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